FastQCFastQC Report
Thu 2 Feb 2017
SRR4064227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843250
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49110.2664315746643158No Hit
TATCAACGCAGAGTACTTTTTTTTT30010.16281025362810253No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19710.10693069306930694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC551.9672449E-413.8125563
GTCTACG655.464684E-513.1509891
CGAACGA1251.4060788E-912.16231216
ACGATTC550.003066319712.0922228
GTCCTAT3700.012.0649461
GTATCAA27250.012.0597651
CGCATCG2600.012.05867413
TCGCGTA1201.0004442E-811.8759689
CGCGTAA1201.000808E-811.87564510
CGAAATT658.0191786E-411.6932613
TCCTATT4200.011.5303922
AATCCTT3150.011.4587626
CGGACCA3400.011.4568159
GGTCATA752.0784013E-411.3975241
CGGTCCA2600.011.32753810
ATCGCCA2700.011.261416
GCGGTCG1900.011.0008969
GTGCTAA1303.2770913E-810.9591571
TTAAATC3650.010.9270563
GCATCGC2900.010.81181214
Produced by FastQC (version 0.11.5)