FastQCFastQC Report
Thu 2 Feb 2017
SRR4064227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843250
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33400.021.9345631
TATACCG750.001291988117.5984365
GTATAAT2700.017.1114481
AACGCAG43650.016.6812236
CCTTATA2301.8189894E-1216.2598672
GGTATCA17150.016.1635861
ATAATAC2051.05501385E-1016.096573
GGCGTTA4400.015.99901442
GTATTAT2550.015.5297181
CCGTTAT850.002980342615.5297179
TTCGCGC850.002981959615.52845417
ATAGCGT2850.015.4372266
AATAGCG3150.015.3637165
GTATTAG4300.015.349141
CGGCGTT4600.015.30340541
GTATTAA2601.8189894E-1215.2310711
CGCGCAA3350.015.10518521
CGCAATA5300.014.9436936
TATCAAC49500.014.9324122
CTAGCGG5250.014.66815129
Produced by FastQC (version 0.11.5)