##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1843250
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29377892309779	32.0	32.0	32.0	32.0	32.0
2	31.396901668249015	32.0	32.0	32.0	32.0	32.0
3	31.465586328495863	32.0	32.0	32.0	32.0	32.0
4	31.55086721822867	32.0	32.0	32.0	32.0	32.0
5	31.508005425200054	32.0	32.0	32.0	32.0	32.0
6	35.07464641258647	36.0	36.0	36.0	36.0	36.0
7	35.09290872100909	36.0	36.0	36.0	36.0	36.0
8	35.0447296894073	36.0	36.0	36.0	36.0	36.0
9	35.13884823002848	36.0	36.0	36.0	36.0	36.0
10	35.00452570188526	36.0	36.0	36.0	36.0	36.0
11	35.138324698223244	36.0	36.0	36.0	36.0	36.0
12	35.04600895158009	36.0	36.0	36.0	36.0	36.0
13	35.09276224060762	36.0	36.0	36.0	36.0	36.0
14	35.03514227587142	36.0	36.0	36.0	36.0	36.0
15	35.00823436864234	36.0	36.0	36.0	36.0	36.0
16	35.02061738776617	36.0	36.0	36.0	36.0	36.0
17	34.989327546453275	36.0	36.0	36.0	36.0	36.0
18	34.98378407703784	36.0	36.0	36.0	36.0	36.0
19	34.975587684795876	36.0	36.0	36.0	36.0	36.0
20	34.96986409873864	36.0	36.0	36.0	36.0	36.0
21	34.96826529228265	36.0	36.0	36.0	36.0	36.0
22	34.95145205479452	36.0	36.0	36.0	36.0	36.0
23	34.901395903973956	36.0	36.0	36.0	32.0	36.0
24	34.8685398074054	36.0	36.0	36.0	32.0	36.0
25	34.84312328767123	36.0	36.0	36.0	32.0	36.0
26	34.77977322663773	36.0	36.0	36.0	32.0	36.0
27	34.775970703919704	36.0	36.0	36.0	32.0	36.0
28	34.7596799131968	36.0	36.0	36.0	32.0	36.0
29	34.71526868303269	36.0	36.0	36.0	32.0	36.0
30	34.69786192865862	36.0	36.0	36.0	32.0	36.0
31	34.68877905872779	36.0	36.0	36.0	32.0	36.0
32	34.65819666350197	36.0	36.0	36.0	32.0	36.0
33	34.63244812152448	36.0	36.0	36.0	32.0	36.0
34	34.60861087752611	36.0	36.0	36.0	32.0	36.0
35	34.570631222026314	36.0	36.0	36.0	32.0	36.0
36	34.55043916994439	36.0	36.0	36.0	32.0	36.0
37	34.551063610470635	36.0	36.0	36.0	32.0	36.0
38	34.50445897192459	36.0	36.0	36.0	32.0	36.0
39	34.49077444730774	36.0	36.0	36.0	32.0	36.0
40	34.47306713685067	36.0	36.0	36.0	32.0	36.0
41	34.44978217821782	36.0	36.0	36.0	32.0	36.0
42	34.392165468601654	36.0	36.0	36.0	32.0	36.0
43	34.38183209005832	36.0	36.0	36.0	32.0	36.0
44	34.30439820968398	36.0	36.0	36.0	32.0	36.0
45	34.28067923504679	36.0	36.0	36.0	32.0	36.0
46	34.25568696595687	36.0	36.0	36.0	32.0	36.0
47	34.232383561643836	36.0	36.0	36.0	32.0	36.0
48	34.183466702834664	36.0	36.0	36.0	32.0	36.0
49	34.145545096975454	36.0	36.0	36.0	32.0	36.0
50	33.56191184049912	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	4.0
20	28.0
21	85.0
22	260.0
23	713.0
24	1873.0
25	4012.0
26	8191.0
27	14252.0
28	23008.0
29	34359.0
30	48164.0
31	67076.0
32	95289.0
33	150242.0
34	309022.0
35	1086668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29050999524708	18.609162074705544	11.90026889829828	26.200059031749095
2	15.835953724862145	21.052648711288192	37.0105618596859	26.100835704163767
3	17.843451917870794	25.412072858582867	28.951000465484185	27.793474758062153
4	12.229974230299742	16.059080428590804	36.58255798182558	35.12838735928388
5	14.386599755865998	36.73587413535874	33.316424793164245	15.561101315611015
6	33.67485060314826	36.34899321576893	16.707545659963813	13.268610521119003
7	29.159256747592565	30.86629594466296	21.847063610470634	18.12738369727384
8	27.537827207378275	33.21524481215245	19.905818527058187	19.341109453411097
9	27.33668107250626	13.85472490199291	18.910024446163646	39.898569579337185
10	15.801786093082782	27.049745529748957	31.60243288859135	25.546035488576912
11	36.93698630136986	21.83746100637461	22.33261901532619	18.892933676929335
12	24.278475518784756	24.087345720873458	29.377729553777293	22.25644920656449
13	29.12574006951893	20.236008461192498	25.498158404851463	25.140093064437114
14	23.38499581716168	19.346739393439616	25.75559637726055	31.51266841213815
15	25.125056424590703	26.989075331168944	22.56186088300144	25.32400736123891
16	25.661541432906205	26.3552681619741	23.4997420293582	24.4834483757615
17	23.610850400108504	26.061847280618473	25.695483520954838	24.63181879831819
18	24.225643852323163	25.650037081423427	26.32835315620692	23.795965910046487
19	25.208517564085177	24.690356706903568	25.95225823952258	24.148867489488676
20	25.808969970795975	24.328044274236817	25.153547640864804	24.709438114102404
21	26.97132781771328	24.565468601654686	24.39115692391157	24.072046656720467
22	25.37640078817392	24.668411412897235	25.545341701170976	24.40984609775787
23	24.33246366574383	24.667527111331015	25.82549249064009	25.174516732285067
24	24.52995811722836	24.683709120895813	25.73175495323452	25.054577808641305
25	24.898044660155197	24.525325125908132	25.581526565896763	24.995103648039912
26	24.888546237102858	25.38004417459109	25.590500129941223	24.140909458364824
27	24.914886679243093	25.015639148817964	25.512402536770313	24.557071635168633
28	24.204572581870266	25.18424879012131	26.077985633368776	24.53319299463964
29	24.335242522343044	24.615463074635237	25.906213734767586	25.143080668254132
30	24.51693199802297	25.077760181253243	25.775743597840023	24.629564222883765
31	25.1680727209198	25.130961352633413	24.814809389176176	24.886156537270608
32	24.433152341393335	25.171063425305018	25.344405526113622	25.051378707188025
33	24.222305773793344	24.939492928770115	25.682125334952282	25.15607596248426
34	24.83432229068456	24.4626833848112	25.94961878528874	24.7533755392155
35	25.542332742873107	24.502779141653487	25.464533052661274	24.490355062812128
36	24.80169366092492	25.54147667960858	24.954696089431547	24.702133570034963
37	25.062406580021758	25.115630535684737	25.417449970295497	24.40451291399801
38	24.305258274804064	25.32777746128116	25.61050841112368	24.756455852791092
39	24.957369385929265	24.498103527693488	25.33107561528875	25.213451471088494
40	25.348103274571493	24.890421727829217	25.26363075580932	24.49784424178997
41	24.652505002191848	25.529461933428532	25.39420744013646	24.42382562424316
42	25.166395038205213	25.650931747109922	25.434081089233747	23.74859212545112
43	24.657488168808843	24.818510639798482	25.61277242392529	24.91122876746738
44	24.235576870910688	24.858562732609236	25.45583272750947	25.450027668970606
45	24.764529049961993	25.065903654526505	25.189111886203552	24.980455409307943
46	24.82134602989972	25.343907536126835	25.016981078846456	24.817765355126983
47	24.404879131205185	25.237888299514378	25.86359461305547	24.493637956224973
48	24.917345104128348	25.769067592010874	25.025526175587537	24.288061128273235
49	24.301624907839063	25.247946147089667	25.224346437230466	25.226082507840804
50	24.666468101866997	25.524745460000315	24.878541818235085	24.930244619897604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	30.0
2	38.0
3	125.0
4	212.0
5	264.0
6	316.0
7	325.0
8	334.0
9	435.5
10	537.0
11	799.5
12	1062.0
13	1904.0
14	2746.0
15	3789.0
16	4832.0
17	5163.5
18	5495.0
19	5659.0
20	5823.0
21	6404.5
22	6986.0
23	8045.0
24	9104.0
25	10347.5
26	11591.0
27	14217.0
28	16843.0
29	20030.5
30	23218.0
31	26762.5
32	30307.0
33	35280.0
34	40253.0
35	47160.0
36	54067.0
37	63594.5
38	73122.0
39	80809.0
40	88496.0
41	95715.0
42	102934.0
43	105400.5
44	107867.0
45	115094.0
46	122321.0
47	127507.0
48	132693.0
49	137249.0
50	141805.0
51	139795.5
52	137786.0
53	135006.0
54	132226.0
55	131136.0
56	130046.0
57	124997.5
58	119949.0
59	109704.0
60	99459.0
61	86722.0
62	73985.0
63	63815.0
64	53645.0
65	45402.5
66	37160.0
67	31669.5
68	26179.0
69	22877.5
70	19576.0
71	15209.5
72	10843.0
73	9129.5
74	7416.0
75	5810.0
76	4204.0
77	3629.0
78	3054.0
79	2534.5
80	2015.0
81	1581.0
82	1147.0
83	983.5
84	820.0
85	579.0
86	338.0
87	249.0
88	160.0
89	119.0
90	78.0
91	66.5
92	55.0
93	43.5
94	32.0
95	30.5
96	29.0
97	21.5
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009439848094398481
2	7.595280075952801E-4
3	4.340160043401601E-4
4	0.0
5	0.0
6	2.712600027126E-4
7	0.0
8	0.0
9	8.680320086803202E-4
10	4.8826800488268E-4
11	0.0
12	0.0
13	5.967720059677201E-4
14	2.1700800217008004E-4
15	0.004448664044486641
16	9.2228400922284E-4
17	0.0
18	4.8826800488268E-4
19	0.0
20	5.967720059677201E-4
21	0.0
22	1.0850400108504002E-4
23	9.2228400922284E-4
24	5.425200054252E-4
25	0.0022243320222433203
26	0.0059134680591346805
27	0.00645598806455988
28	0.004882680048826801
29	0.00320086803200868
30	0.00450291604502916
31	0.00808354808083548
32	0.00401464804014648
33	0.004177404041774041
34	0.0034178760341787603
35	0.0029838600298386004
36	0.007703784077037841
37	0.0051539400515394004
38	0.0084090600840906
39	0.00493693204936932
40	0.0025498440254984405
41	0.0031466160314661605
42	0.00249559202495592
43	0.0015733080157330802
44	0.0016275600162756003
45	0.0015733080157330802
46	0.0011935440119354402
47	0.00287535602875356
48	0.0023870880238708804
49	3.7976400379764004E-4
50	0.00124779601247796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1843250.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.9917105974062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.67794125795929	45.626683998313005
2	12.986528720289344	15.062220304238473
3	3.734450121445342	6.497014520499202
4	1.5328732133690044	3.5557575908884558
5	0.8230607708807637	2.3865351014497644
6	0.48912224859444553	1.7019021532344993
7	0.31823335310657136	1.2918427561058925
8	0.2342909106356034	1.0869544548146135
9	0.16861451394116228	0.8800419685498377
>10	0.9081181293110564	9.377619460911873
>50	0.06604557063126276	2.6529262508480644
>100	0.05356718700189726	6.517356419916975
>500	0.005459633741972762	2.1721323751391166
>1k	0.0016943690923363743	1.1910126450902276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0850400108504E-4
2	0.0	0.0	0.0	0.0	1.0850400108504E-4
3	0.0	0.0	0.0	0.0	1.0850400108504E-4
4	0.0	0.0	0.0	1.0850400108504E-4	1.0850400108504E-4
5	0.0	0.0	0.0	1.0850400108504E-4	1.6275600162756002E-4
6	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
7	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
8	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
9	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
10	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
11	0.0	0.0	0.0	1.0850400108504E-4	2.712600027126E-4
12	0.0	0.0	0.0	1.0850400108504E-4	7.052760070527601E-4
13	0.0	0.0	0.0	1.0850400108504E-4	7.052760070527601E-4
14	0.0	0.0	0.0	1.0850400108504E-4	7.595280075952801E-4
15	0.0	0.0	0.0	1.0850400108504E-4	9.765360097653601E-4
16	0.0	0.0	0.0	2.1700800217008E-4	0.0011392920113929201
17	0.0	0.0	0.0	2.712600027126E-4	0.0011392920113929201
18	0.0	0.0	0.0	2.712600027126E-4	0.00124779601247796
19	5.425200054252E-5	0.0	0.0	2.712600027126E-4	0.00124779601247796
20	5.425200054252E-5	0.0	0.0	4.8826800488268007E-4	0.001356300013563
21	5.425200054252E-5	0.0	0.0	7.595280075952801E-4	0.0014105520141055202
22	1.0850400108504E-4	0.0	0.0	9.2228400922284E-4	0.0014105520141055202
23	1.0850400108504E-4	0.0	0.0	0.001356300013563	0.00146480401464804
24	1.0850400108504E-4	0.0	0.0	0.0016818120168181202	0.00146480401464804
25	1.0850400108504E-4	0.0	0.0	0.0021700800217008	0.00146480401464804
26	1.0850400108504E-4	0.0	0.0	0.002712600027126	0.0015733080157330802
27	1.0850400108504E-4	0.0	0.0	0.0035263800352638005	0.0016275600162756001
28	1.0850400108504E-4	0.0	0.0	0.007649532076495321	0.0016275600162756001
29	1.0850400108504E-4	0.0	0.0	0.01415977214159772	0.0016275600162756001
30	1.0850400108504E-4	0.0	0.0	0.026420724264207242	0.0016275600162756001
31	1.0850400108504E-4	0.0	0.0	0.05674759256747593	0.0016275600162756001
32	1.0850400108504E-4	0.0	0.0	0.08739997287399973	0.0016275600162756001
33	1.0850400108504E-4	0.0	0.0	0.11978841719788418	0.0016275600162756001
34	1.0850400108504E-4	0.0	0.0	0.15575749355757493	0.0016818120168181202
35	1.0850400108504E-4	0.0	0.0	0.19503594195035942	0.0016818120168181202
36	1.0850400108504E-4	0.0	0.0	0.2529770785297708	0.0016818120168181202
37	1.0850400108504E-4	0.0	0.0	0.33668791536687914	0.0016818120168181202
38	1.0850400108504E-4	0.0	0.0	0.44649396446493966	0.00173606401736064
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3340	0.0	21.934563	1
TATACCG	75	0.0012919881	17.598436	5
GTATAAT	270	0.0	17.111448	1
AACGCAG	4365	0.0	16.681223	6
CCTTATA	230	1.8189894E-12	16.259867	2
GGTATCA	1715	0.0	16.163586	1
ATAATAC	205	1.05501385E-10	16.09657	3
GGCGTTA	440	0.0	15.999014	42
GTATTAT	255	0.0	15.529718	1
CCGTTAT	85	0.0029803426	15.529717	9
TTCGCGC	85	0.0029819596	15.528454	17
ATAGCGT	285	0.0	15.437226	6
AATAGCG	315	0.0	15.363716	5
GTATTAG	430	0.0	15.34914	1
CGGCGTT	460	0.0	15.303405	41
GTATTAA	260	1.8189894E-12	15.231071	1
CGCGCAA	335	0.0	15.105185	21
CGCAATA	530	0.0	14.94369	36
TATCAAC	4950	0.0	14.932412	2
CTAGCGG	525	0.0	14.668151	29
>>END_MODULE