FastQCFastQC Report
Thu 2 Feb 2017
SRR4064226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367434
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27440.20066782016536083No Hit
TATCAACGCAGAGTACTTTTTTTTT18350.13419294825198144No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14790.10815878499437634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAT551.9632769E-413.815361
GTTATTC1450.013.75379853
GCGTAAC801.9960862E-613.064136511
CCGATAA801.9977288E-613.063189
CGTTATT1251.03682396E-1012.9149992
AAGACGG2850.012.6650085
CGAACGA853.9352E-612.29610716
TCGCGTA853.9400347E-612.2947589
TTCCGAT853.9432616E-612.2938587
AATTCCG853.9481038E-612.2925075
GTATTAC1251.4133548E-912.1575171
CGTCGTA1650.012.0919810
TGCACCG550.003071131412.0893255
GATAACG951.0378844E-612.00150411
ATAACGA951.0383428E-612.00106312
GCGTTAT1353.7289283E-1011.9605211
CAAGACG2950.011.9119494
ACGAACG802.8610222E-511.87692315
GTATCAA17900.011.6205321
TTTATAC1157.104063E-811.5611643
Produced by FastQC (version 0.11.5)