##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064226_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1367434
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214221673587172	32.0	32.0	32.0	32.0	32.0
2	30.79095590719552	32.0	32.0	32.0	32.0	32.0
3	30.818157220019394	32.0	32.0	32.0	32.0	32.0
4	30.846393317703086	32.0	32.0	32.0	32.0	32.0
5	30.748788606982128	32.0	32.0	32.0	32.0	32.0
6	34.399033518253894	36.0	36.0	36.0	32.0	36.0
7	34.331336649520196	36.0	36.0	36.0	32.0	36.0
8	34.28988455749967	36.0	36.0	36.0	32.0	36.0
9	34.4463542664582	36.0	36.0	36.0	32.0	36.0
10	34.12170751933914	36.0	36.0	36.0	32.0	36.0
11	34.41815180842366	36.0	36.0	36.0	32.0	36.0
12	34.21603309556439	36.0	36.0	36.0	32.0	36.0
13	34.317678220667325	36.0	36.0	36.0	32.0	36.0
14	34.20179255452183	36.0	36.0	36.0	32.0	36.0
15	34.14952385270514	36.0	36.0	36.0	32.0	36.0
16	34.14684803800403	36.0	36.0	36.0	32.0	36.0
17	34.06648584136419	36.0	36.0	36.0	32.0	36.0
18	34.052186065287245	36.0	36.0	36.0	32.0	36.0
19	34.07807689438759	36.0	36.0	36.0	32.0	36.0
20	34.05878748078518	36.0	36.0	36.0	32.0	36.0
21	34.0546446848623	36.0	36.0	36.0	32.0	36.0
22	34.023429284338405	36.0	36.0	36.0	32.0	36.0
23	33.964741991203965	36.0	36.0	36.0	32.0	36.0
24	33.9443212615746	36.0	36.0	36.0	32.0	36.0
25	33.53852324865405	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	14.0
5	20.0
6	59.0
7	19.0
8	54.0
9	95.0
10	135.0
11	33.0
12	86.0
13	59.0
14	122.0
15	239.0
16	432.0
17	563.0
18	729.0
19	1002.0
20	1436.0
21	2127.0
22	3437.0
23	5168.0
24	7773.0
25	11249.0
26	15994.0
27	21209.0
28	28784.0
29	38609.0
30	50468.0
31	68043.0
32	95935.0
33	134167.0
34	282951.0
35	596421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.637472437914894	17.155285555638468	11.063314883013854	26.143927123432785
2	16.791334580500028	19.98484677840461	37.094676918981456	26.129141722113904
3	18.899383142645064	23.863713649477297	28.557753132735858	28.67915007514178
4	12.611737655379606	15.34580441238956	36.148333634396366	35.894124297834466
5	14.538305814620218	36.62924043671086	33.832731666218095	14.999722082450823
6	34.00153153133385	35.67487944859021	16.881585712728896	13.442003307347036
7	30.066951011579825	30.498588637858155	21.13510235758555	18.29935799297647
8	27.970922466250077	32.86943493839642	19.693759757009317	19.465882838344186
9	27.454680209864446	14.104977586119857	18.50178557448966	39.93855662952603
10	15.47534726897028	26.868345108966068	32.23769704916498	25.418610572898682
11	37.15935356840514	21.112926510002538	22.65722691762585	19.07049300396648
12	24.598321206927967	23.67396787740464	29.079005427429262	22.64870548823813
13	29.41552673134309	19.509102164645345	25.27261125802906	25.802759845982504
14	23.388480069313943	19.53545314576418	25.46636166189548	31.609705123026398
15	24.944993110876474	27.194586458683183	22.300857500144513	25.559562930295826
16	25.62692317543019	25.74342049899199	23.756087387628014	24.87356893794981
17	23.91852199835797	25.885590684047	25.342345467078196	24.85354185051683
18	24.716917059918732	25.014929917212147	26.038946591880467	24.229206430988654
19	25.519782090369265	24.95173582701886	25.454649241312083	24.07383284129979
20	25.710555263213745	24.43336717016952	24.974951714810363	24.881125851806367
21	26.498753310660934	24.35658462009091	24.584991865599058	24.5596702036491
22	25.61610280077666	24.377273815091215	24.943079149074446	25.063544235057684
23	24.367751483187615	24.247281371631995	25.44363887071347	25.941328274466922
24	24.704091452994582	24.898546163782537	25.27786824357703	25.119494139645855
25	24.808747728501913	24.603535303636082	25.401331538822397	25.18638542903961
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	205.0
2	341.0
3	477.0
4	477.0
5	477.0
6	1405.0
7	2333.0
8	2333.0
9	2333.0
10	2529.0
11	2725.0
12	2725.0
13	2725.0
14	3305.5
15	3886.0
16	3886.0
17	3886.0
18	6758.0
19	9630.0
20	9630.0
21	9630.0
22	14706.5
23	19783.0
24	19783.0
25	19783.0
26	29261.5
27	38740.0
28	38740.0
29	38740.0
30	48336.0
31	57932.0
32	57932.0
33	57932.0
34	72299.5
35	86667.0
36	86667.0
37	86667.0
38	99017.0
39	111367.0
40	111367.0
41	111367.0
42	127609.0
43	143851.0
44	143851.0
45	143851.0
46	160533.0
47	177215.0
48	177215.0
49	177215.0
50	181526.0
51	185837.0
52	185837.0
53	185837.0
54	174825.0
55	163813.0
56	163813.0
57	163813.0
58	149859.5
59	135906.0
60	135906.0
61	135906.0
62	118025.5
63	100145.0
64	100145.0
65	100145.0
66	81440.5
67	62736.0
68	62736.0
69	62736.0
70	46911.0
71	31086.0
72	31086.0
73	31086.0
74	23546.5
75	16007.0
76	16007.0
77	16007.0
78	12731.5
79	9456.0
80	9456.0
81	9456.0
82	6585.0
83	3714.0
84	3714.0
85	3714.0
86	2850.0
87	1986.0
88	1986.0
89	1986.0
90	1424.0
91	862.0
92	862.0
93	862.0
94	593.0
95	324.0
96	324.0
97	324.0
98	537.5
99	751.0
100	751.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03802742947740074
2	0.004972817700890866
3	0.0013894637693665653
4	0.004168391308099696
5	0.008921819992774789
6	0.012797692612586786
7	0.023035846702656217
8	0.03422468652966067
9	0.04731489783053515
10	0.056309847495381864
11	0.053457790284576805
12	0.0626721289656393
13	0.06113640585212888
14	0.06567044552058819
15	0.05755305192060458
16	0.06428098175122163
17	0.06084388716384118
18	0.07678615567552072
19	0.0728371533836368
20	0.07846813813317499
21	0.07422661715300337
22	0.07773684141245574
23	0.08190523272055543
24	0.0767130260034488
25	0.07642050731516109
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1367434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.04087916894917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29874264428247	45.803108765910615
2	11.78834582179987	13.448352192461464
3	3.39714948336081	5.8132917959772605
4	1.3781442693003427	3.1444224297016232
5	0.7861399962322265	2.242105826748029
6	0.46877114361441025	1.6043470896479815
7	0.33390817450816734	1.3332491084951337
8	0.22811446028551513	1.0409479492668896
9	0.1856874597237652	0.9532598357963166
>10	0.9847200584376434	9.982924334618724
>50	0.07495203875874837	3.006682881950101
>100	0.06721261654134315	7.844722186982297
>500	0.006437962821240676	2.5138895576956743
>1k	0.0016738703335225761	1.2686960447480158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2744	0.20066782016536083	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1835	0.13419294825198144	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1479	0.10815878499437634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4625934414384898E-4
2	0.0	0.0	0.0	0.0	1.4625934414384898E-4
3	0.0	0.0	0.0	0.0	1.4625934414384898E-4
4	0.0	0.0	0.0	0.0	1.4625934414384898E-4
5	0.0	0.0	0.0	0.0	1.4625934414384898E-4
6	0.0	0.0	0.0	0.0	1.4625934414384898E-4
7	0.0	0.0	0.0	0.0	1.4625934414384898E-4
8	0.0	0.0	0.0	0.0	1.4625934414384898E-4
9	0.0	0.0	0.0	0.0	1.4625934414384898E-4
10	0.0	7.312967207192449E-5	0.0	0.0	1.4625934414384898E-4
11	0.0	1.4625934414384898E-4	0.0	0.0	1.4625934414384898E-4
12	0.0	1.4625934414384898E-4	0.0	0.0	4.38778032431547E-4
13	0.0	1.4625934414384898E-4	0.0	7.312967207192449E-5	5.850373765753959E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGTAT	55	1.9632769E-4	13.81536	1
GTTATTC	145	0.0	13.7537985	3
GCGTAAC	80	1.9960862E-6	13.0641365	11
CCGATAA	80	1.9977288E-6	13.06318	9
CGTTATT	125	1.03682396E-10	12.914999	2
AAGACGG	285	0.0	12.665008	5
CGAACGA	85	3.9352E-6	12.296107	16
TCGCGTA	85	3.9400347E-6	12.294758	9
TTCCGAT	85	3.9432616E-6	12.293858	7
AATTCCG	85	3.9481038E-6	12.292507	5
GTATTAC	125	1.4133548E-9	12.157517	1
CGTCGTA	165	0.0	12.09198	10
TGCACCG	55	0.0030711314	12.089325	5
GATAACG	95	1.0378844E-6	12.001504	11
ATAACGA	95	1.0383428E-6	12.001063	12
GCGTTAT	135	3.7289283E-10	11.960521	1
CAAGACG	295	0.0	11.911949	4
ACGAACG	80	2.8610222E-5	11.876923	15
GTATCAA	1790	0.0	11.620532	1
TTTATAC	115	7.104063E-8	11.561164	3
>>END_MODULE