FastQCFastQC Report
Thu 2 Feb 2017
SRR4064226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367434
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT407.034998E-427.4984028
GTATCAA20250.022.3789441
GTATCGT755.4742202E-520.53288810
TACGACT550.004485415819.9988374
CTACACG1303.590685E-818.6143024
TCTATAC1303.5937774E-818.6129423
TAATACT2650.018.263094
GTAAACG3400.018.12123127
TAAACGC3700.017.24665628
CGCGGGA3450.017.2176544
TCGATCG902.2160047E-417.1107441
ATCGTTT4050.016.84283829
GTATTAG3400.016.822551
AACGCAG26400.016.6656976
GGTCGGA4150.016.4333838
CAACGGA1351.0863587E-616.29534714
GTATAGG1501.8393075E-716.1323951
TACCGTC3700.016.053127
CGTCGTA3600.015.88854510
TTAACGG1259.4341085E-615.84139635
Produced by FastQC (version 0.11.5)