##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1367434
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316052548057165	32.0	32.0	32.0	32.0	32.0
2	31.389321897802745	32.0	32.0	32.0	32.0	32.0
3	31.46184093711287	32.0	32.0	32.0	32.0	32.0
4	31.545757235815405	32.0	32.0	32.0	32.0	32.0
5	31.491247840846434	32.0	32.0	32.0	32.0	32.0
6	35.075213136429255	36.0	36.0	36.0	36.0	36.0
7	35.08832601792847	36.0	36.0	36.0	36.0	36.0
8	35.03722300308461	36.0	36.0	36.0	36.0	36.0
9	35.143214955895495	36.0	36.0	36.0	36.0	36.0
10	34.999890305491896	36.0	36.0	36.0	36.0	36.0
11	35.14841008779948	36.0	36.0	36.0	36.0	36.0
12	35.044348758331296	36.0	36.0	36.0	36.0	36.0
13	35.09320596094583	36.0	36.0	36.0	36.0	36.0
14	35.03528799196159	36.0	36.0	36.0	36.0	36.0
15	35.007774415437964	36.0	36.0	36.0	36.0	36.0
16	35.022904213292925	36.0	36.0	36.0	36.0	36.0
17	34.98379373337214	36.0	36.0	36.0	36.0	36.0
18	34.983297182898774	36.0	36.0	36.0	36.0	36.0
19	34.98157863560508	36.0	36.0	36.0	36.0	36.0
20	34.97803623428992	36.0	36.0	36.0	36.0	36.0
21	34.966869333364535	36.0	36.0	36.0	36.0	36.0
22	34.95668456393508	36.0	36.0	36.0	36.0	36.0
23	34.90354269383385	36.0	36.0	36.0	32.0	36.0
24	34.871963838839754	36.0	36.0	36.0	32.0	36.0
25	34.8540777836444	36.0	36.0	36.0	32.0	36.0
26	34.78485762384145	36.0	36.0	36.0	32.0	36.0
27	34.77858821705472	36.0	36.0	36.0	32.0	36.0
28	34.76273882322657	36.0	36.0	36.0	32.0	36.0
29	34.734840584627854	36.0	36.0	36.0	32.0	36.0
30	34.71031435520837	36.0	36.0	36.0	32.0	36.0
31	34.7079120454808	36.0	36.0	36.0	32.0	36.0
32	34.668963913432016	36.0	36.0	36.0	32.0	36.0
33	34.642525343087854	36.0	36.0	36.0	32.0	36.0
34	34.63028051079613	36.0	36.0	36.0	32.0	36.0
35	34.595832778766656	36.0	36.0	36.0	32.0	36.0
36	34.56174630731721	36.0	36.0	36.0	32.0	36.0
37	34.55721592413235	36.0	36.0	36.0	32.0	36.0
38	34.5210467196223	36.0	36.0	36.0	32.0	36.0
39	34.52464543078496	36.0	36.0	36.0	32.0	36.0
40	34.49990346883286	36.0	36.0	36.0	32.0	36.0
41	34.48791605298683	36.0	36.0	36.0	32.0	36.0
42	34.42138560252268	36.0	36.0	36.0	32.0	36.0
43	34.41409238032695	36.0	36.0	36.0	32.0	36.0
44	34.35679235707171	36.0	36.0	36.0	32.0	36.0
45	34.33005395507205	36.0	36.0	36.0	32.0	36.0
46	34.30577709783434	36.0	36.0	36.0	32.0	36.0
47	34.272715173090624	36.0	36.0	36.0	32.0	36.0
48	34.24023316664643	36.0	36.0	36.0	32.0	36.0
49	34.22514139622095	36.0	36.0	36.0	32.0	36.0
50	33.62698236258569	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	13.0
21	46.0
22	139.0
23	403.0
24	1080.0
25	2566.0
26	5306.0
27	9522.0
28	15675.0
29	24605.0
30	35465.0
31	49900.0
32	71879.0
33	114828.0
34	238339.0
35	797665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48682415803843	18.112165978089877	11.680280959809496	26.720728904062202
2	16.127541353092038	20.63548361855187	36.604772014090656	26.63220301426544
3	17.955095259128818	24.518731516394283	28.95779521846854	28.56837800600836
4	12.269696380227492	16.026001986201894	36.24942776031604	35.45487387325458
5	14.136696908223726	37.016192371990165	33.61185987769794	15.235250842088174
6	34.08025706598724	35.97110201538506	16.64712881308088	13.301512105546825
7	29.886217460014496	30.631994401188212	21.13544137080208	18.346346767995215
8	27.92456528066437	33.05746383372068	19.51392169567233	19.504049189942624
9	27.440442411087773	13.855183791911166	18.425336547180958	40.2790372498201
10	15.275836775945375	27.030153844353716	32.34000355413585	25.35400582556506
11	37.3882303557103	21.056680656383165	22.489950147466093	19.065138840440447
12	24.50306377881411	23.699731393732325	29.093064429670573	22.704140397782993
13	29.46638428697421	19.461455318240255	25.282026230304233	25.790134164481294
14	23.40862287850511	19.484332676140387	25.421446916555997	31.68559752879851
15	25.090959885502073	27.13913882288584	22.325121164052508	25.444780127559586
16	25.601694859477163	25.7579735401253	23.718598918115674	24.921732682281856
17	23.994284184830857	25.923664323104447	25.259281252331007	24.82277023973369
18	24.76664219249569	24.985885911360597	25.978259915695745	24.269211980447967
19	25.50872656376834	24.954696168151443	25.446566342507204	24.090010925573008
20	25.83282568744273	24.26169517406099	25.00886704406756	24.896612094428715
21	26.51703848229604	24.396277992210226	24.514309282934313	24.572374242559423
22	25.627819152981644	24.36728115182327	25.03093389653014	24.973965798664942
23	24.36437005959385	24.14848953103758	25.551201054830873	25.935939354537695
24	24.788544934658447	24.78635103807873	25.342869470466496	25.082234556796323
25	24.89052343983104	24.425046949867486	25.506502776038893	25.177926834262582
26	24.808134764210898	25.11200366251611	25.589783569285768	24.49007800398723
27	25.245146078394086	24.79441956254004	24.909459771762567	25.050974587303305
28	24.52255338319031	24.61799151368532	25.685655319889598	25.173799783234774
29	24.597373990601128	24.83848886717693	25.496965770024527	25.067171372197418
30	24.596658302794598	24.985209037077233	25.59820212097655	24.819930539151624
31	25.170604845462837	24.66963252034423	24.875945356717892	25.28381727747504
32	24.79186473401688	24.821702818528866	24.803054015708874	25.58337843174538
33	24.59875292716925	24.513771773678076	25.37389513530433	25.513580163848342
34	25.208791626687884	24.503214879127565	25.449322505909063	24.838670988275492
35	25.68630949856149	24.47063228671348	25.38683297912952	24.456225235595507
36	24.83282930414718	24.945093120458708	25.01969187787745	25.202385697516664
37	25.509275003016707	24.76632404187555	24.901106857249424	24.82329409785832
38	24.777558533956057	24.637138473195126	25.464519799959923	25.120783192888897
39	25.195447052680386	24.638907456460466	24.87124993966555	25.2943955511936
40	25.43209425117558	24.72404033903511	25.31859500808097	24.525270401708337
41	24.65381051596951	24.923010091510303	25.60789301053762	24.81528638198257
42	25.60366300473453	25.12077647977698	25.087647963071575	24.18791255241692
43	24.870888053250724	24.46303909996797	25.447741656172436	25.218331190608872
44	24.716435549146052	24.85457952017458	25.037040720659583	25.39194421001979
45	24.68632465443972	25.097830435046514	25.22595521336944	24.989889697144324
46	24.620947023630617	24.821104838963276	25.208037612410518	25.349910524995593
47	24.80675031921849	24.856406528584866	25.393629671449947	24.9432134807467
48	25.475865216132178	25.23577523540958	24.641144826254713	24.647214722203532
49	24.666381948060305	25.17858292039385	24.919045334612616	25.23598979693323
50	24.765286884586526	25.368688634166393	24.846608269331746	25.019416211915342
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	70.0
2	49.0
3	148.5
4	248.0
5	283.5
6	319.0
7	298.5
8	278.0
9	350.0
10	422.0
11	661.5
12	901.0
13	1466.5
14	2032.0
15	2597.0
16	3162.0
17	3341.5
18	3521.0
19	3536.0
20	3551.0
21	4054.5
22	4558.0
23	5424.0
24	6290.0
25	7624.5
26	8959.0
27	10928.0
28	12897.0
29	14922.5
30	16948.0
31	19077.0
32	21206.0
33	24568.5
34	27931.0
35	32220.5
36	36510.0
37	42676.5
38	48843.0
39	54376.5
40	59910.0
41	65399.5
42	70889.0
43	72744.0
44	74599.0
45	82080.5
46	89562.0
47	95458.0
48	101354.0
49	104632.0
50	107910.0
51	105773.5
52	103637.0
53	102760.0
54	101883.0
55	101153.5
56	100424.0
57	96378.0
58	92332.0
59	84341.0
60	76350.0
61	67046.5
62	57743.0
63	49931.5
64	42120.0
65	35363.0
66	28606.0
67	24381.0
68	20156.0
69	18266.5
70	16377.0
71	12657.5
72	8938.0
73	7588.5
74	6239.0
75	4769.0
76	3299.0
77	2872.5
78	2446.0
79	2061.0
80	1676.0
81	1339.5
82	1003.0
83	836.0
84	669.0
85	476.5
86	284.0
87	209.0
88	134.0
89	100.5
90	67.0
91	53.5
92	40.0
93	33.5
94	27.0
95	23.5
96	20.0
97	16.0
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008848690320702865
2	2.193890162157735E-4
3	4.38778032431547E-4
4	0.0
5	0.0
6	2.193890162157735E-4
7	7.312967207192449E-5
8	0.0
9	0.0013163340972946408
10	9.506857369350185E-4
11	7.312967207192449E-5
12	3.6564836035962245E-4
13	5.850373765753959E-4
14	1.4625934414384898E-4
15	0.005338466061250488
16	3.6564836035962245E-4
17	0.0
18	4.38778032431547E-4
19	0.0
20	8.044263927911694E-4
21	0.0
22	1.4625934414384898E-4
23	8.044263927911694E-4
24	2.9251868828769796E-4
25	0.0013163340972946408
26	0.004972817700890866
27	0.006289151798185507
28	0.0040952616360277715
29	0.0026326681945892817
30	0.0032908352432366025
31	0.006801059502688977
32	0.0039490022918839225
33	0.005265336389178563
34	0.003071446227020829
35	0.003510224259452376
36	0.008702430976559015
37	0.0035833539315243005
38	0.007898004583767845
39	0.0040952616360277715
40	0.001755112129726188
41	0.003437094587380451
42	0.0023401495063015837
43	0.0011700747531507918
44	0.0014625934414384898
45	0.0012432044252227166
46	9.506857369350185E-4
47	0.0023401495063015837
48	0.0021938901621577347
49	1.4625934414384898E-4
50	0.0014625934414384898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1367434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.94144438586066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10656138415757	48.61644438536876
2	11.576648876440156	13.878421096035481
3	3.147969841258243	5.660815775044428
4	1.3105266306899253	3.1421943659875806
5	0.6972823636493973	2.0898056010965913
6	0.4651305910213445	1.6728359672321054
7	0.3062637333375869	1.2850523377482719
8	0.22433267305166554	1.0757459556526299
9	0.17007167149181304	0.917490747450319
>10	0.8668128792477013	9.201866471183619
>50	0.06452429495738342	2.680330515730297
>100	0.057119084646876504	6.8332225611786255
>500	0.005896124553080058	2.365940887338027
>1k	8.598514973241751E-4	0.5798333329532558
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4625934414384898E-4
2	0.0	0.0	0.0	0.0	1.4625934414384898E-4
3	0.0	0.0	0.0	0.0	1.4625934414384898E-4
4	0.0	0.0	0.0	0.0	1.4625934414384898E-4
5	0.0	0.0	0.0	0.0	2.193890162157735E-4
6	0.0	0.0	0.0	0.0	3.6564836035962245E-4
7	0.0	0.0	0.0	0.0	3.6564836035962245E-4
8	0.0	0.0	0.0	0.0	3.6564836035962245E-4
9	0.0	0.0	0.0	0.0	3.6564836035962245E-4
10	0.0	0.0	0.0	0.0	3.6564836035962245E-4
11	0.0	0.0	0.0	0.0	3.6564836035962245E-4
12	0.0	0.0	0.0	0.0	6.581670486473204E-4
13	0.0	0.0	0.0	7.312967207192449E-5	6.581670486473204E-4
14	0.0	0.0	0.0	7.312967207192449E-5	6.581670486473204E-4
15	0.0	0.0	0.0	7.312967207192449E-5	8.044263927911694E-4
16	0.0	0.0	0.0	7.312967207192449E-5	8.044263927911694E-4
17	0.0	0.0	0.0	7.312967207192449E-5	8.77556064863094E-4
18	0.0	0.0	0.0	7.312967207192449E-5	8.77556064863094E-4
19	0.0	0.0	0.0	1.4625934414384898E-4	8.77556064863094E-4
20	0.0	0.0	0.0	2.193890162157735E-4	8.77556064863094E-4
21	0.0	0.0	0.0	2.9251868828769796E-4	8.77556064863094E-4
22	0.0	0.0	0.0	3.6564836035962245E-4	9.506857369350185E-4
23	0.0	0.0	0.0	8.77556064863094E-4	9.506857369350185E-4
24	0.0	0.0	0.0	0.0013894637693665653	9.506857369350185E-4
25	0.0	0.0	0.0	0.0013894637693665653	0.0010238154090069429
26	0.0	0.0	0.0	0.0021207604900858102	0.0010238154090069429
27	0.0	0.0	0.0	0.002705797866661206	0.0010238154090069429
28	0.0	0.0	0.0	0.004607169340531243	0.0010238154090069429
29	0.0	0.0	0.0	0.010530672778357127	0.0010238154090069429
30	0.0	0.0	0.0	0.023182106046800065	0.0010969450810788674
31	0.0	0.0	0.0	0.04782680553503862	0.0010969450810788674
32	0.0	0.0	0.0	0.07737119305209611	0.0010969450810788674
33	0.0	0.0	0.0	0.10011452106646464	0.0010969450810788674
34	0.0	0.0	0.0	0.13134089104117638	0.0011700747531507918
35	0.0	0.0	0.0	0.16505366986633357	0.0011700747531507918
36	0.0	0.0	0.0	0.21383116113830722	0.0011700747531507918
37	0.0	0.0	0.0	0.28081794075619004	0.0011700747531507918
38	0.0	0.0	0.0	0.376764070514555	0.0011700747531507918
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	40	7.034998E-4	27.498402	8
GTATCAA	2025	0.0	22.378944	1
GTATCGT	75	5.4742202E-5	20.532888	10
TACGACT	55	0.0044854158	19.998837	4
CTACACG	130	3.590685E-8	18.614302	4
TCTATAC	130	3.5937774E-8	18.612942	3
TAATACT	265	0.0	18.26309	4
GTAAACG	340	0.0	18.121231	27
TAAACGC	370	0.0	17.246656	28
CGCGGGA	345	0.0	17.21765	44
TCGATCG	90	2.2160047E-4	17.11074	41
ATCGTTT	405	0.0	16.842838	29
GTATTAG	340	0.0	16.82255	1
AACGCAG	2640	0.0	16.665697	6
GGTCGGA	415	0.0	16.43338	38
CAACGGA	135	1.0863587E-6	16.295347	14
GTATAGG	150	1.8393075E-7	16.132395	1
TACCGTC	370	0.0	16.05312	7
CGTCGTA	360	0.0	15.888545	10
TTAACGG	125	9.4341085E-6	15.841396	35
>>END_MODULE