FastQCFastQC Report
Thu 2 Feb 2017
SRR4064225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866051
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31770.3668375188066292No Hit
TATCAACGCAGAGTACTTTTTTTTT23660.27319407286637853No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22820.2634948750131343No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15720.18151355982499875No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12880.14872103374974452No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11930.13775170284428978No Hit
GTACATGGGGTGGTATCAACGCAAA11810.13636610315096917No Hit
GAATAGGACCGCGGTTCTATTTTGT11680.13486503681653852No Hit
GCGCAAGACGGACCAGAGCGAAAGC11190.12920717140214605No Hit
GTATCTGATCGTCTTCGAACCTCCG11080.1279370383499355No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11040.1274751717854953No Hit
GGTATCAACGCAGAGTACTTTTTTT10990.12689783857994505No Hit
GAACTACGACGGTATCTGATCGTCT10680.12331837270553352No Hit
GTCCTATTCCATTATTCCTAGCTGC10590.12227917293554307No Hit
CTATTGGAGCTGGAATTACCGCGGC10490.12112450652444257No Hit
GAATAACGCCGCCGCATCGCCAGTC10280.11869970706113149No Hit
GATTAAGAGGGACGGCCGGGGGCAT9950.11488930790449985No Hit
CTTTAATATACGCTATTGGAGCTGG9150.10565197661569585No Hit
GCTTTGAACACTCTAATTTTTTCAA8960.10345811043460489No Hit
GTACATGGGGAATAATTGCAATCCC8720.10068691104796368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG307.71595E-419.0010979
CGCGGTC1004.0017767E-1115.210
ATAAGTC456.7639694E-414.776075
GTTATTC1300.014.6111623
GCGTTAT1250.014.4358281
GTACTAC400.005286724314.2458841
AACCGCG1152.5465852E-1114.0418567
TAGAACC1301.8189894E-1213.8838134
CGTTATT1301.8189894E-1213.8781992
ATTACAC1101.8735591E-1013.814193
CGGACAT1051.369699E-913.569865
GAACCGC1400.013.569866
CGACCAT1550.013.48387110
TAGGAGG852.6962334E-713.410994
TCCAACG500.001496001413.30307618
TAGACTG655.4529504E-513.1523275
TCGGCGT1102.73576E-912.95529413
CGTCGTA1403.6379788E-1212.89285810
ATAGAAC2000.012.8212963
CCAACGA604.080802E-412.66959619
Produced by FastQC (version 0.11.5)