FastQCFastQC Report
Thu 2 Feb 2017
SRR4064225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866051
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11870.13705890299762946No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11470.13244023735322746No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10830.12505037232218427No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10460.1207781066011124No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10320.1191615736255717No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10040.11592850767449031No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA9970.11512024118671994No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9670.11165624195341844No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9220.10646024310346619No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.10472824348681543No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT8900.1027653105879446No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8880.1025343773057245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG301.3014837E-436.6635365
GTAATAC508.343586E-526.3977493
GTCCTAG1053.0686351E-923.0469841
GTCTTAC703.2146803E-521.9993921
ATATCCG500.0025811321.9981255
ATATAGG654.933447E-420.305963
TATAAGC654.933447E-420.305963
ATAGCGC550.004484815519.9982958
CTACACG550.004484815519.9982954
ATCGTTT2600.019.46212629
GACTTAT708.12229E-418.8566231
ACGGGAT951.5966367E-518.52580543
GTATAGT600.007411627618.3328291
GTATAGA851.4305413E-418.1171471
CTAAGAC1102.7968435E-617.9984663
CTATACA1254.852882E-717.59854
TTAGACC1002.4835146E-517.59854
CGTGCGG750.001291207617.59849710
TACACGG750.001291207617.5984975
CGTTTAT2650.017.43549531
Produced by FastQC (version 0.11.5)