##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866051
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24609405219785	32.0	32.0	32.0	32.0	32.0
2	31.35981945635996	32.0	32.0	32.0	32.0	32.0
3	31.447552164941786	32.0	32.0	32.0	32.0	32.0
4	31.544234692876056	32.0	32.0	32.0	32.0	32.0
5	31.478215486154973	32.0	32.0	32.0	32.0	32.0
6	35.06087401319322	36.0	36.0	36.0	36.0	36.0
7	35.09555326418421	36.0	36.0	36.0	36.0	36.0
8	35.034651538997124	36.0	36.0	36.0	36.0	36.0
9	35.16343379315999	36.0	36.0	36.0	36.0	36.0
10	34.99657295009185	36.0	36.0	36.0	36.0	36.0
11	35.15438236316337	36.0	36.0	36.0	36.0	36.0
12	35.04797754404764	36.0	36.0	36.0	36.0	36.0
13	35.09561908016964	36.0	36.0	36.0	36.0	36.0
14	35.038659386110055	36.0	36.0	36.0	36.0	36.0
15	35.00537959080932	36.0	36.0	36.0	36.0	36.0
16	35.01984871560682	36.0	36.0	36.0	36.0	36.0
17	34.979427308553426	36.0	36.0	36.0	36.0	36.0
18	34.9759817839827	36.0	36.0	36.0	36.0	36.0
19	34.97471742426254	36.0	36.0	36.0	36.0	36.0
20	34.963883189327184	36.0	36.0	36.0	36.0	36.0
21	34.958360419882894	36.0	36.0	36.0	36.0	36.0
22	34.93997120261971	36.0	36.0	36.0	36.0	36.0
23	34.88411767898195	36.0	36.0	36.0	32.0	36.0
24	34.84303118407576	36.0	36.0	36.0	32.0	36.0
25	34.82852164595388	36.0	36.0	36.0	32.0	36.0
26	34.760346677043266	36.0	36.0	36.0	32.0	36.0
27	34.76172996740377	36.0	36.0	36.0	32.0	36.0
28	34.72806451352172	36.0	36.0	36.0	32.0	36.0
29	34.685700957564855	36.0	36.0	36.0	32.0	36.0
30	34.68070818000326	36.0	36.0	36.0	32.0	36.0
31	34.66574716731463	36.0	36.0	36.0	32.0	36.0
32	34.621205910506426	36.0	36.0	36.0	32.0	36.0
33	34.58121634869078	36.0	36.0	36.0	32.0	36.0
34	34.563927528517375	36.0	36.0	36.0	32.0	36.0
35	34.50741122635965	36.0	36.0	36.0	32.0	36.0
36	34.47475148692167	36.0	36.0	36.0	32.0	36.0
37	34.452778185118426	36.0	36.0	36.0	32.0	36.0
38	34.405423006266375	36.0	36.0	36.0	32.0	36.0
39	34.396170664314226	36.0	36.0	36.0	32.0	36.0
40	34.3539883909839	36.0	36.0	36.0	32.0	36.0
41	34.3325912677198	36.0	36.0	36.0	32.0	36.0
42	34.229613498512215	36.0	36.0	36.0	32.0	36.0
43	34.22993680510732	36.0	36.0	36.0	32.0	36.0
44	34.16237496406101	36.0	36.0	36.0	32.0	36.0
45	34.09825056492054	36.0	36.0	36.0	32.0	36.0
46	34.07856119327845	36.0	36.0	36.0	32.0	36.0
47	34.03435478972947	36.0	36.0	36.0	32.0	36.0
48	33.97979795647139	36.0	36.0	36.0	32.0	36.0
49	33.95120148813407	36.0	36.0	36.0	32.0	36.0
50	33.32135289954056	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	6.0
20	10.0
21	25.0
22	106.0
23	262.0
24	759.0
25	1663.0
26	3431.0
27	6428.0
28	10647.0
29	16631.0
30	23652.0
31	33453.0
32	48656.0
33	78200.0
34	159753.0
35	482366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94254496829673	18.067688299871016	11.872531030636294	26.117235701195963
2	15.84494072465224	20.700126899010787	36.61273170038901	26.842200675947964
3	17.978333764410287	24.781767292344075	28.826462274608332	28.413436668637303
4	11.991210679278703	15.522757897629585	35.98448590210045	36.50154552099126
5	14.167756864203149	37.07125792822825	33.03870095410086	15.722284253467752
6	34.3868533997499	35.75917759849616	16.489367229798777	13.364601771955167
7	30.05334564978927	30.73852548929046	20.856763466312568	18.3513653946077
8	28.617483265997034	32.30687338274536	19.462941558868934	19.61270179238867
9	27.73572182411477	13.610540828273917	18.502682317947627	40.15105502966369
10	15.9117597815356	26.44331414649354	31.386937168391942	26.257988903578916
11	37.73611484773991	20.777413801265745	22.42315983700729	19.063311513987053
12	24.952254488770855	23.735350152993476	28.650655273944924	22.661740084290745
13	29.439626255849856	19.051160040967755	25.738556914347605	25.770656788834785
14	23.870270469997042	19.662189624593555	25.155072236180903	31.312467669228493
15	25.24110743137445	26.960845446441358	22.624491341841377	25.173555780342816
16	25.837828850133015	25.434271541531185	23.886089454626074	24.841810153709726
17	23.823077393825535	25.629091127427827	25.440303169212896	25.107528309533734
18	24.99826799438368	24.36204459798995	26.667921408513156	23.971765999113213
19	25.648604989775432	24.745193989730396	25.686824447982854	23.919376572511318
20	25.873135922498253	23.782136032192323	25.277208459144735	25.067519586164693
21	27.10429293424983	23.769962738914913	24.652358810277917	24.47338551655734
22	25.71927057413478	24.022141883099263	25.22980748246928	25.02878006029668
23	24.225272098710693	23.659482290779014	26.014553499467695	26.1006921110426
24	24.71964602423884	24.91374612030742	25.47214473100798	24.89446312444576
25	24.98328589491187	24.018544285161685	25.652427442308912	25.34574237761753
26	24.487670252137715	25.135133574674217	26.017240284297255	24.359955888890813
27	25.40496348746657	24.564205246165145	25.074018133865433	24.95681313250285
28	24.511037876810875	24.410578071125542	26.03144507388992	25.046938978173667
29	24.261140767478302	24.540230084142483	26.089481785294062	25.109147363085153
30	24.341585644673334	24.8936514168264	26.20332093947022	24.561441999030045
31	24.90857337513496	24.702567566787337	24.9933313702735	25.395527687804204
32	24.534018766266907	24.856181482687507	24.900637628143397	25.70916212290219
33	24.35445318183393	24.311497557764923	25.611482546390917	25.72256671401023
34	25.153459241502585	24.5673350053347	25.63635355900733	24.642852194155385
35	25.97216945891987	24.365175681589605	25.526802282145937	24.135852577344586
36	24.57017352414407	25.380230002921518	24.98934743974808	25.06024903318633
37	25.53116556199626	25.041915891087967	25.003233181681715	24.42368536523406
38	24.775750577367205	24.693071593533485	25.445150115473442	25.086027713625864
39	25.380135056489085	24.586724372061227	24.665822948517796	25.36731762293189
40	25.564614743318092	25.024566038476593	25.36866378071044	24.042155437494873
41	24.246696666008482	25.325772298298304	25.856476590005535	24.571054445687682
42	25.983014543292132	25.54007632485985	24.883636342642042	23.59327278920598
43	24.8900738537428	24.424735230406124	25.484275480465012	25.200915435386058
44	24.665229475278885	25.14649999249458	24.723540802340764	25.464729729885764
45	24.648197136618556	25.451971562481596	24.991484217223473	24.90834708367637
46	24.622939765046038	25.11021405428374	24.89579027345094	25.37105590721928
47	24.813776874037995	24.998874174250112	25.466986747587555	24.72036220412433
48	25.970689372472673	25.738943274744408	23.95506411988444	24.33530323289848
49	24.517663631797422	25.795888204423555	24.606227159096814	25.08022100468221
50	24.68826461625862	25.92608416018216	24.302369754710814	25.0832814688484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	28.0
2	27.0
3	116.5
4	206.0
5	233.0
6	260.0
7	250.5
8	241.0
9	339.0
10	437.0
11	638.5
12	840.0
13	1419.0
14	1998.0
15	2663.5
16	3329.0
17	3650.5
18	3972.0
19	3983.0
20	3994.0
21	4330.0
22	4666.0
23	5003.0
24	5340.0
25	5586.5
26	5833.0
27	6810.5
28	7788.0
29	9016.0
30	10244.0
31	11350.5
32	12457.0
33	14455.5
34	16454.0
35	19003.5
36	21553.0
37	26080.0
38	30607.0
39	33203.0
40	35799.0
41	39480.0
42	43161.0
43	44263.5
44	45366.0
45	50436.0
46	55506.0
47	59598.5
48	63691.0
49	65635.0
50	67579.0
51	64468.5
52	61358.0
53	61233.0
54	61108.0
55	61723.5
56	62339.0
57	61327.0
58	60315.0
59	55344.0
60	50373.0
61	43688.5
62	37004.0
63	32209.0
64	27414.0
65	23588.5
66	19763.0
67	17047.0
68	14331.0
69	13057.5
70	11784.0
71	9156.5
72	6529.0
73	5605.0
74	4681.0
75	3667.5
76	2654.0
77	2346.5
78	2039.0
79	1652.0
80	1265.0
81	1019.0
82	773.0
83	638.5
84	504.0
85	358.0
86	212.0
87	156.0
88	100.0
89	69.0
90	38.0
91	36.5
92	35.0
93	24.0
94	13.0
95	17.0
96	21.0
97	13.0
98	5.0
99	10.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006004265337722605
2	9.237331288804009E-4
3	3.463999233301503E-4
4	0.0
5	0.0
6	2.3093328222010021E-4
7	1.1546664111005011E-4
8	0.0
9	0.001039199769990451
10	6.927998466603006E-4
11	0.0
12	1.1546664111005011E-4
13	4.6186656444020043E-4
14	3.463999233301503E-4
15	0.005426932132172354
16	3.463999233301503E-4
17	0.0
18	3.463999233301503E-4
19	0.0
20	6.927998466603006E-4
21	0.0
22	0.0
23	5.773332055502505E-4
24	3.463999233301503E-4
25	0.0018474662577608017
26	0.006466131902162805
27	0.005426932132172354
28	0.0038103991566316533
29	0.002309332822201002
30	0.003579465874411553
31	0.006466131902162805
32	0.0033485325921914525
33	0.004734132285512054
34	0.0026557327455311525
35	0.0027711993866412024
36	0.007158931748823106
37	0.003579465874411553
38	0.005888798696612556
39	0.004965065567732154
40	0.002078399539980902
41	0.0036949325155216034
42	0.0018474662577608017
43	0.0017319996166507516
44	0.001154666411100501
45	0.0013855996933206012
46	0.001039199769990451
47	0.002309332822201002
48	0.0019629328988708516
49	6.927998466603006E-4
50	0.0013855996933206012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	866051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.888845470458236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.227469034908	40.84471675861847
2	13.322824381878611	14.092575999264623
3	4.229431001043263	6.710691679264278
4	1.7706889008094302	3.7459876660466205
5	0.9314609624930745	2.4631947453530256
6	0.5558244326013808	1.7638147514735734
7	0.3498058514177297	1.2950579334206065
8	0.2556636326235659	1.0817403486595025
9	0.17928919387924214	0.8534158622642037
>10	0.9782894857903051	9.470390638259891
>50	0.09282486142516244	3.4674372613812046
>100	0.09655347402260231	10.312085909992371
>500	0.008558148826646527	3.1446221537897987
>1k	0.0013166382810225427	0.7542682922118435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1187	0.13705890299762946	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1147	0.13244023735322746	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1083	0.12505037232218427	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1046	0.1207781066011124	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1032	0.1191615736255717	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1004	0.11592850767449031	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	997	0.11512024118671994	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	967	0.11165624195341844	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	922	0.10646024310346619	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	907	0.10472824348681543	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	890	0.1027653105879446	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	888	0.1025343773057245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.1546664111005011E-4	0.0
8	0.0	0.0	0.0	1.1546664111005011E-4	0.0
9	0.0	0.0	0.0	1.1546664111005011E-4	0.0
10	0.0	0.0	0.0	1.1546664111005011E-4	0.0
11	0.0	0.0	0.0	1.1546664111005011E-4	0.0
12	0.0	0.0	0.0	2.3093328222010021E-4	2.3093328222010021E-4
13	0.0	0.0	0.0	2.3093328222010021E-4	2.3093328222010021E-4
14	0.0	0.0	0.0	2.3093328222010021E-4	2.3093328222010021E-4
15	0.0	0.0	0.0	2.3093328222010021E-4	3.463999233301503E-4
16	0.0	0.0	0.0	3.463999233301503E-4	4.6186656444020043E-4
17	0.0	0.0	0.0	3.463999233301503E-4	4.6186656444020043E-4
18	0.0	0.0	0.0	4.6186656444020043E-4	4.6186656444020043E-4
19	0.0	0.0	0.0	5.773332055502505E-4	4.6186656444020043E-4
20	0.0	0.0	0.0	5.773332055502505E-4	4.6186656444020043E-4
21	0.0	0.0	0.0	6.927998466603006E-4	4.6186656444020043E-4
22	0.0	0.0	0.0	9.237331288804009E-4	4.6186656444020043E-4
23	0.0	0.0	0.0	0.0013855996933206012	4.6186656444020043E-4
24	0.0	0.0	0.0	0.0030021326688613025	4.6186656444020043E-4
25	0.0	0.0	0.0	0.004156799079961804	4.6186656444020043E-4
26	0.0	0.0	0.0	0.005080532208842204	4.6186656444020043E-4
27	0.0	0.0	0.0	0.006235198619942706	4.6186656444020043E-4
28	0.0	0.0	0.0	0.010853864264344709	4.6186656444020043E-4
29	0.0	0.0	0.0	0.020553062117588918	4.6186656444020043E-4
30	0.0	0.0	0.0	0.03648745859077583	4.6186656444020043E-4
31	0.0	0.0	0.0	0.06373758589274765	4.6186656444020043E-4
32	0.0	0.0	0.0	0.08960211350139888	4.6186656444020043E-4
33	0.0	0.0	0.0	0.1168522408033707	4.6186656444020043E-4
34	0.0	0.0	0.0	0.1481437005441943	4.6186656444020043E-4
35	0.0	0.0	0.0	0.18844155829160175	4.6186656444020043E-4
36	0.0	0.0	0.0	0.23797674732781327	4.6186656444020043E-4
37	0.0	0.0	0.0	0.3125681974849056	4.6186656444020043E-4
38	0.0	0.0	0.0	0.40482604373183567	4.6186656444020043E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	30	1.3014837E-4	36.663536	5
GTAATAC	50	8.343586E-5	26.397749	3
GTCCTAG	105	3.0686351E-9	23.046984	1
GTCTTAC	70	3.2146803E-5	21.999392	1
ATATCCG	50	0.00258113	21.998125	5
ATATAGG	65	4.933447E-4	20.30596	3
TATAAGC	65	4.933447E-4	20.30596	3
ATAGCGC	55	0.0044848155	19.998295	8
CTACACG	55	0.0044848155	19.998295	4
ATCGTTT	260	0.0	19.462126	29
GACTTAT	70	8.12229E-4	18.856623	1
ACGGGAT	95	1.5966367E-5	18.525805	43
GTATAGT	60	0.0074116276	18.332829	1
GTATAGA	85	1.4305413E-4	18.117147	1
CTAAGAC	110	2.7968435E-6	17.998466	3
CTATACA	125	4.852882E-7	17.5985	4
TTAGACC	100	2.4835146E-5	17.5985	4
CGTGCGG	75	0.0012912076	17.598497	10
TACACGG	75	0.0012912076	17.598497	5
CGTTTAT	265	0.0	17.435495	31
>>END_MODULE