FastQCFastQC Report
Thu 2 Feb 2017
SRR4064224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417523
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36940.26059541891031046No Hit
TATCAACGCAGAGTACTTTTTTTTT24870.17544688869245859No Hit
GCGCAAGACGGACCAGAGCGAAAGC16960.11964532497885395No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16550.11675295568396421No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14920.10525402409696351No Hit
GAACTACGACGGTATCTGATCGTCT14520.10243220039463205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA350.002170799816.2848975
TAGGTTG500.001499999813.2993345
ATCGGAC500.001501596513.2974563
CTTATAC655.4616197E-513.151333
ACGAACG1450.013.10556615
CGTTATT1102.755769E-912.9511522
CGATAAC1403.6379788E-1212.8922110
CCGACCA2000.012.8248099
TTCGCAG604.0860512E-412.668715515
CGAACGA1501.8189894E-1212.66871516
GCGTTAT1155.338734E-912.3884951
AACGAAC1551.8189894E-1212.25961314
CGACCAT2050.012.04817610
GTATCAA19350.011.8784451
TAACGAA1603.6379788E-1211.87524313
GCGTAAC1201.0009899E-811.87482411
CGCGTAA1201.0015356E-811.87440410
CGCCCCT1459.822543E-1111.7925115
ACGCGCG658.0212764E-411.69254713
CTAAACA658.028406E-411.6913084
Produced by FastQC (version 0.11.5)