##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064224_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1417523 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20586614820359 32.0 32.0 32.0 32.0 32.0 2 30.85799313309202 32.0 32.0 32.0 32.0 32.0 3 30.852884926734873 32.0 32.0 32.0 32.0 32.0 4 30.88191232170483 32.0 32.0 32.0 32.0 32.0 5 30.82129390493135 32.0 32.0 32.0 32.0 32.0 6 34.422448171916784 36.0 36.0 36.0 32.0 36.0 7 34.37648278017358 36.0 36.0 36.0 32.0 36.0 8 34.35161052060531 36.0 36.0 36.0 32.0 36.0 9 34.4599840708052 36.0 36.0 36.0 32.0 36.0 10 34.196736843070624 36.0 36.0 36.0 32.0 36.0 11 34.431971826912154 36.0 36.0 36.0 32.0 36.0 12 34.268382241416894 36.0 36.0 36.0 32.0 36.0 13 34.33912747800212 36.0 36.0 36.0 32.0 36.0 14 34.24973069220041 36.0 36.0 36.0 32.0 36.0 15 34.1940779796871 36.0 36.0 36.0 32.0 36.0 16 34.18241326595759 36.0 36.0 36.0 32.0 36.0 17 34.121048476814835 36.0 36.0 36.0 32.0 36.0 18 34.11730462221777 36.0 36.0 36.0 32.0 36.0 19 34.13101374721962 36.0 36.0 36.0 32.0 36.0 20 34.10824162994181 36.0 36.0 36.0 32.0 36.0 21 34.09833420692292 36.0 36.0 36.0 32.0 36.0 22 34.07307676841928 36.0 36.0 36.0 32.0 36.0 23 34.02284195741445 36.0 36.0 36.0 32.0 36.0 24 34.0041473753865 36.0 36.0 36.0 32.0 36.0 25 33.62402303172506 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 11.0 5 17.0 6 49.0 7 16.0 8 98.0 9 92.0 10 117.0 11 44.0 12 87.0 13 93.0 14 124.0 15 203.0 16 393.0 17 503.0 18 629.0 19 825.0 20 1297.0 21 1960.0 22 3030.0 23 4992.0 24 7531.0 25 11087.0 26 16129.0 27 21651.0 28 29449.0 29 39582.0 30 51883.0 31 70207.0 32 97388.0 33 136228.0 34 288259.0 35 633539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.37109241301634 16.978389139454023 11.219045051618927 26.43147339591071 2 17.323331270931206 19.31777054565234 36.161505134958 27.19739304845846 3 19.045949433259988 22.739089364109734 28.448465001238098 29.76649620139218 4 13.310513268043115 14.523311317520138 34.78178665308374 37.384388761353 5 15.316489275388495 35.49711653922621 33.16374981127336 16.022644374111927 6 35.27666534020834 34.34821506687326 16.206710185788832 14.168409407129573 7 31.039091259982033 30.05191328357693 20.249003119595308 18.65999233684573 8 28.76416895905875 32.11941233840929 19.17507759317886 19.9413411093531 9 27.274799763979818 14.200876888373182 18.533442309158204 39.990881038488794 10 16.325352231978993 26.055707710986248 30.95857921337211 26.66036084366265 11 37.74268777995649 20.87351366307673 21.910402629138204 19.473395927828573 12 24.804268226826494 23.179611564816494 28.08414754409656 23.93197266426045 13 29.280129548162666 18.9925145558147 25.242973395754387 26.484382500268243 14 23.751122443793097 19.09538089671258 24.701387594666485 32.45210906482783 15 25.350575645704364 26.544219895953187 21.950489528259933 26.154714930082516 16 26.02609174467364 25.56510935095373 23.102700947378825 25.3060979569938 17 24.267762047019094 25.64357595848134 24.843429694426717 25.24523230007285 18 25.23559196629277 24.502623493397493 25.668934336417138 24.592850203892596 19 25.7151026315325 24.622672479217776 25.230731216576363 24.431493672673362 20 25.807255557030338 23.944792587425624 24.72444302614226 25.52350882940178 21 26.65567682814905 23.891603010321504 24.42843426570464 25.024285895824804 22 26.093261424697495 23.732815033884556 24.77889788884284 25.395025652575104 23 24.68906034658051 23.6931214839249 25.46224384898669 26.155574320507895 24 24.898317948355896 24.524348271175672 25.07291243800413 25.5044213424643 25 25.135850860218167 24.02285160786562 25.05685015210637 25.784447379809848 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 95.0 2 283.0 3 471.0 4 471.0 5 471.0 6 1439.5 7 2408.0 8 2408.0 9 2408.0 10 2574.0 11 2740.0 12 2740.0 13 2740.0 14 3038.0 15 3336.0 16 3336.0 17 3336.0 18 5632.5 19 7929.0 20 7929.0 21 7929.0 22 12491.5 23 17054.0 24 17054.0 25 17054.0 26 25724.0 27 34394.0 28 34394.0 29 34394.0 30 42826.0 31 51258.0 32 51258.0 33 51258.0 34 65391.0 35 79524.0 36 79524.0 37 79524.0 38 93794.0 39 108064.0 40 108064.0 41 108064.0 42 125435.5 43 142807.0 44 142807.0 45 142807.0 46 162524.0 47 182241.0 48 182241.0 49 182241.0 50 188585.0 51 194929.0 52 194929.0 53 194929.0 54 184661.0 55 174393.0 56 174393.0 57 174393.0 58 162110.0 59 149827.0 60 149827.0 61 149827.0 62 131667.5 63 113508.0 64 113508.0 65 113508.0 66 93291.5 67 73075.0 68 73075.0 69 73075.0 70 55587.0 71 38099.0 72 38099.0 73 38099.0 74 29019.5 75 19940.0 76 19940.0 77 19940.0 78 16185.0 79 12430.0 80 12430.0 81 12430.0 82 8514.0 83 4598.0 84 4598.0 85 4598.0 86 3493.0 87 2388.0 88 2388.0 89 2388.0 90 1675.5 91 963.0 92 963.0 93 963.0 94 651.5 95 340.0 96 340.0 97 340.0 98 526.0 99 712.0 100 712.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035554978649376415 2 0.005784738589779495 3 0.002116367776748596 4 0.0040210987758223325 5 0.009100381440018963 6 0.015167302400031604 7 0.024832048580516863 8 0.03647207135263414 9 0.049029186828009136 10 0.05946993452663555 11 0.05537829015825493 12 0.06461976278339046 13 0.06398485245036589 14 0.06849977037409623 15 0.06017539045221842 16 0.06934631748479567 17 0.06398485245036589 18 0.07696524148109062 19 0.07393178100108429 20 0.07894051807272263 21 0.07498996488945858 22 0.07731796944388204 23 0.08260888888575353 24 0.07745906062899861 25 0.07618923996294946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1417523.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.44666385283028 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01800992368312 41.69802350857868 2 12.557013291523308 13.422609367751361 3 3.776924826600867 6.055920948142375 4 1.7785766837450108 3.8023596061040483 5 0.9812112765462939 2.622123463308813 6 0.6161570002075157 1.9758921642395622 7 0.4149144886247074 1.5523056640835597 8 0.30844016019229126 1.3188078048408411 9 0.22486402332250272 1.081640868040151 >10 1.1637025092305104 10.785481659520054 >50 0.07712242292414703 2.8806367613755812 >100 0.0736425019445904 8.353123649782368 >500 0.006767119214359331 2.3882932427721606 >1k 0.0026537722409252277 2.0627812914604573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3694 0.26059541891031046 No Hit TATCAACGCAGAGTACTTTTTTTTT 2487 0.17544688869245859 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1696 0.11964532497885395 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1655 0.11675295568396421 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1492 0.10525402409696351 No Hit GAACTACGACGGTATCTGATCGTCT 1452 0.10243220039463205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.054559255828653E-5 10 0.0 0.0 0.0 0.0 7.054559255828653E-5 11 0.0 0.0 0.0 0.0 7.054559255828653E-5 12 0.0 0.0 0.0 0.0 7.054559255828653E-5 13 0.0 0.0 0.0 0.0 7.054559255828653E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTA 35 0.0021707998 16.284897 5 TAGGTTG 50 0.0014999998 13.299334 5 ATCGGAC 50 0.0015015965 13.297456 3 CTTATAC 65 5.4616197E-5 13.15133 3 ACGAACG 145 0.0 13.105566 15 CGTTATT 110 2.755769E-9 12.951152 2 CGATAAC 140 3.6379788E-12 12.89221 10 CCGACCA 200 0.0 12.824809 9 TTCGCAG 60 4.0860512E-4 12.6687155 15 CGAACGA 150 1.8189894E-12 12.668715 16 GCGTTAT 115 5.338734E-9 12.388495 1 AACGAAC 155 1.8189894E-12 12.259613 14 CGACCAT 205 0.0 12.048176 10 GTATCAA 1935 0.0 11.878445 1 TAACGAA 160 3.6379788E-12 11.875243 13 GCGTAAC 120 1.0009899E-8 11.874824 11 CGCGTAA 120 1.0015356E-8 11.874404 10 CGCCCCT 145 9.822543E-11 11.792511 5 ACGCGCG 65 8.0212764E-4 11.692547 13 CTAAACA 65 8.028406E-4 11.691308 4 >>END_MODULE