FastQCFastQC Report
Thu 2 Feb 2017
SRR4064224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417523
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16720.11795223075745508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.021.305561
TACTAAT755.4788285E-520.5307332
TATACAG1553.45608E-1119.868455
TATACCG901.0032563E-519.5530785
ATACCGG901.0032563E-519.5530786
CGCAATA4400.018.50157236
CGTTCGG600.007404560718.33683427
TCTAGCG4450.018.29304528
CTATACT1355.5355486E-817.9236554
CTAGCGG4800.016.95917529
GTATAAT1702.4720066E-916.8219931
TCTAGAC1702.4720066E-916.8213983
CAATACG4600.016.73996438
ATACGAA4750.016.67333840
GTACTAC1602.1324013E-816.4984911
CTAGACT2007.2759576E-1116.497914
ACTATAC1206.415823E-616.497913
AACGCAG26400.016.2479426
GCGAAAG6250.016.19166218
GGACCGT1653.1164745E-815.9979736
Produced by FastQC (version 0.11.5)