##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064224_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1417523 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.313935646899555 32.0 32.0 32.0 32.0 32.0 2 31.383109127682584 32.0 32.0 32.0 32.0 32.0 3 31.44554338800852 32.0 32.0 32.0 32.0 32.0 4 31.526373117049953 32.0 32.0 32.0 32.0 32.0 5 31.48212762685332 32.0 32.0 32.0 32.0 32.0 6 35.055992036813514 36.0 36.0 36.0 36.0 36.0 7 35.06484974141513 36.0 36.0 36.0 36.0 36.0 8 35.007085599316554 36.0 36.0 36.0 36.0 36.0 9 35.11403695037047 36.0 36.0 36.0 36.0 36.0 10 34.966231941210125 36.0 36.0 36.0 36.0 36.0 11 35.107229300688594 36.0 36.0 36.0 36.0 36.0 12 35.0069931845903 36.0 36.0 36.0 36.0 36.0 13 35.05460158318419 36.0 36.0 36.0 36.0 36.0 14 34.99451225835489 36.0 36.0 36.0 36.0 36.0 15 34.974095658412594 36.0 36.0 36.0 36.0 36.0 16 34.97794462594258 36.0 36.0 36.0 36.0 36.0 17 34.93939851416873 36.0 36.0 36.0 36.0 36.0 18 34.93795232952129 36.0 36.0 36.0 36.0 36.0 19 34.93276440664455 36.0 36.0 36.0 36.0 36.0 20 34.935879700011924 36.0 36.0 36.0 32.0 36.0 21 34.92234623353554 36.0 36.0 36.0 32.0 36.0 22 34.91811138161427 36.0 36.0 36.0 32.0 36.0 23 34.85268246088423 36.0 36.0 36.0 32.0 36.0 24 34.8246596351523 36.0 36.0 36.0 32.0 36.0 25 34.791247126148924 36.0 36.0 36.0 32.0 36.0 26 34.73443958228544 36.0 36.0 36.0 32.0 36.0 27 34.72925941942388 36.0 36.0 36.0 32.0 36.0 28 34.71710370837016 36.0 36.0 36.0 32.0 36.0 29 34.68494338363469 36.0 36.0 36.0 32.0 36.0 30 34.666474547502936 36.0 36.0 36.0 32.0 36.0 31 34.65589553044289 36.0 36.0 36.0 32.0 36.0 32 34.61927672425774 36.0 36.0 36.0 32.0 36.0 33 34.59696668061118 36.0 36.0 36.0 32.0 36.0 34 34.59288420716983 36.0 36.0 36.0 32.0 36.0 35 34.54777806074399 36.0 36.0 36.0 32.0 36.0 36 34.522571415066984 36.0 36.0 36.0 32.0 36.0 37 34.51823286112465 36.0 36.0 36.0 32.0 36.0 38 34.47645435029978 36.0 36.0 36.0 32.0 36.0 39 34.48808661305672 36.0 36.0 36.0 32.0 36.0 40 34.45812025624981 36.0 36.0 36.0 32.0 36.0 41 34.441800944323305 36.0 36.0 36.0 32.0 36.0 42 34.378220318118295 36.0 36.0 36.0 32.0 36.0 43 34.3701767096548 36.0 36.0 36.0 32.0 36.0 44 34.307968900680976 36.0 36.0 36.0 32.0 36.0 45 34.27011907390568 36.0 36.0 36.0 32.0 36.0 46 34.25762333309583 36.0 36.0 36.0 32.0 36.0 47 34.22214595459827 36.0 36.0 36.0 32.0 36.0 48 34.18090217936499 36.0 36.0 36.0 32.0 36.0 49 34.170114347350975 36.0 36.0 36.0 32.0 36.0 50 33.55022387643798 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 0.0 19 4.0 20 13.0 21 50.0 22 133.0 23 491.0 24 1252.0 25 2757.0 26 5656.0 27 10341.0 28 17355.0 29 26535.0 30 38448.0 31 54089.0 32 77638.0 33 122332.0 34 248324.0 35 812103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.559315815763554 17.884839522617533 11.753737134858572 26.802107526760338 2 16.440754729759284 20.094334165306304 35.686843304545846 27.778067800388566 3 18.354450025396467 23.303374908290536 28.711340933461255 29.630834132851742 4 12.918167818088314 15.241728000180595 34.71689700978397 37.12320717194712 5 14.881240022207754 36.095498979558 32.87008394220059 16.15317705603366 6 35.46044755534831 34.63746267256334 15.939071182619259 13.963018589469096 7 30.991048444500567 30.220335670985715 20.158601048733736 18.630014835779978 8 28.82274220594657 32.39510046750564 18.948616706748318 19.833540619799468 9 27.424056437389773 14.079506172839507 18.318306878306878 40.17813051146384 10 16.335075357599393 26.25374953792148 30.92165970139146 26.489515403087672 11 37.9914653821707 20.85217785133342 21.782181710182776 19.3741750563131 12 24.903669925405037 23.24859861081521 27.984962490881976 23.862768972897776 13 29.40064747201613 18.914182797618082 25.12851733987625 26.55665239048954 14 23.819769738698575 19.095321406399908 24.668646650488178 32.41626220441334 15 25.585444489124992 26.717179199559776 21.780425688021616 25.916950623293616 16 26.25988706942042 25.477880900848533 23.035566642304666 25.226665387426383 17 24.483553353278925 25.69975936898378 24.576955717826095 25.2397315599112 18 25.343487217111576 24.618770810993848 25.486906710310965 24.55083526158361 19 25.857710950721785 24.716988719054296 25.025696232089356 24.399604098134564 20 26.138154543799917 23.87807104550643 24.542120924378878 25.441653486314774 21 26.99547026750183 23.929276632548465 24.20045389034252 24.874799209607183 22 26.25832439753936 23.7241802584796 24.696230035555054 25.321265308425982 23 24.84779605871157 23.72660150997597 25.32468998367555 26.100912447636905 24 25.15962771522317 24.50037636232934 24.94178200331989 25.3982139191276 25 25.31363382074177 24.000544621963925 24.96668423284141 25.71913732445289 26 25.131434505684915 24.80358901565926 25.24396163213013 24.8210148465257 27 25.708982312216783 24.310524975324867 24.533959259753114 25.446533452705232 28 25.065487642183847 24.174869818398342 25.409981212648407 25.3496613267694 29 25.06157049018646 24.450698104595407 25.299033990579062 25.188697414639066 30 25.023316255910196 24.665424073663846 25.238277320232022 25.07298235019394 31 25.384789050373925 24.397911669253563 24.585226471003246 25.632072809369266 32 25.20268649556249 24.440407489452966 24.407955074570005 25.948950940414544 33 24.90091305323251 24.243461108108832 24.90444053442751 25.951185304231146 34 25.710794567477247 24.150753846837624 24.989435350340713 25.149016235344412 35 26.08160714058953 24.086226625673557 24.915800924743593 24.91636530899332 36 25.02717999044732 24.72148005895289 24.714001593054338 25.53733835754545 37 25.81351788945681 24.327050946943643 24.738774775918813 25.12065638768074 38 25.088965209734955 24.178500474104844 25.20876134465165 25.523772971508556 39 25.729737890495752 24.28327834063865 24.40878443921914 25.578199329646463 40 25.71960501707609 24.3607136331473 25.036420292646312 24.883261057130298 41 24.86835718800795 24.700734829329964 25.291363780025282 25.139544202636806 42 25.87954268718545 24.710511055394786 24.693085986717442 24.716860270702323 43 25.3415157911371 23.928256992572862 24.99428573041064 25.735941485879405 44 25.059717560278404 24.44652791593122 24.730477331312883 25.763277192477492 45 25.023844931752702 24.627722766689335 24.86998162952595 25.47845067203201 46 24.99731922398589 24.377001763668428 24.97537918871252 25.650299823633155 47 25.09024774124913 24.23662439232247 25.35677575879003 25.31635210763838 48 25.806738351659046 24.904938137478855 24.38232717083199 24.90599634003011 49 24.83908470874403 24.885786121638134 24.639510241859703 25.635618927758134 50 24.966455168443733 25.017812975747546 24.709807224801867 25.305924631006853 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 25.0 2 29.0 3 116.0 4 203.0 5 235.5 6 268.0 7 256.0 8 244.0 9 331.5 10 419.0 11 645.0 12 871.0 13 1518.5 14 2166.0 15 2716.5 16 3267.0 17 3412.0 18 3557.0 19 3653.0 20 3749.0 21 4156.5 22 4564.0 23 5199.0 24 5834.0 25 6694.5 26 7555.0 27 8949.0 28 10343.0 29 11924.5 30 13506.0 31 15416.5 32 17327.0 33 20191.0 34 23055.0 35 27010.0 36 30965.0 37 38007.0 38 45049.0 39 50851.0 40 56653.0 41 62935.5 42 69218.0 43 72507.5 44 75797.0 45 83392.5 46 90988.0 47 97642.5 48 104297.0 49 108482.5 50 112668.0 51 110563.5 52 108459.0 53 107367.5 54 106276.0 55 107556.5 56 108837.0 57 105649.0 58 102461.0 59 94356.5 60 86252.0 61 76274.0 62 66296.0 63 57630.0 64 48964.0 65 41396.5 66 33829.0 67 28953.5 68 24078.0 69 21428.0 70 18778.0 71 14688.0 72 10598.0 73 9104.5 74 7611.0 75 6056.0 76 4501.0 77 3849.5 78 3198.0 79 2639.0 80 2080.0 81 1664.0 82 1248.0 83 1008.5 84 769.0 85 547.0 86 325.0 87 230.0 88 135.0 89 104.0 90 73.0 91 68.5 92 64.0 93 44.0 94 24.0 95 23.0 96 22.0 97 21.0 98 20.0 99 16.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008959290254902389 2 6.349103330245788E-4 3 2.116367776748596E-4 4 0.0 5 0.0 6 0.0 7 2.8218237023314613E-4 8 0.0 9 0.0016225486288405904 10 0.001058183888374298 11 1.4109118511657307E-4 12 7.054559255828653E-5 13 7.054559255828654E-4 14 2.116367776748596E-4 15 0.003738916405589186 16 0.001058183888374298 17 0.0 18 2.116367776748596E-4 19 0.0 20 6.349103330245788E-4 21 0.0 22 2.116367776748596E-4 23 0.001199275073490871 24 4.232735553497192E-4 25 0.0018341854065154499 26 0.0061374665525709285 27 0.0073367416260617995 28 0.005643647404662923 29 0.0029629148874480344 30 0.0040210987758223325 31 0.008677107884669244 32 0.004444372331172052 33 0.005714192997221209 34 0.004232735553497192 35 0.00345673403535604 36 0.008465471106994384 37 0.004797100293963484 38 0.00811274314420295 39 0.0043738267386137656 40 0.003104006072564608 41 0.003597825220472613 42 0.0017636398139571634 43 0.001340366258607444 44 0.001199275073490871 45 0.0021869133693068828 46 0.0016225486288405904 47 0.002539641332098315 48 0.0017636398139571634 49 4.938191479080057E-4 50 0.001199275073490871 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1417523.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.97716746185455 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21688688048705 45.13553829596299 2 12.129383421152331 13.821958207920773 3 3.628545908834065 6.202328036719972 4 1.6009188941197496 3.6486329569243177 5 0.9027376942446099 2.571771838955179 6 0.5586731456066979 1.9098968024204324 7 0.37400415639369367 1.4916788215191186 8 0.2603956654903728 1.1869285951188826 9 0.19585925100013743 1.004355480886941 >10 0.9975981499897412 9.878422412786424 >50 0.06721277652049953 2.6691694956690295 >100 0.06041897899108284 7.178307297365244 >500 0.005991927189132844 2.311085196280808 >1k 0.0013731499808429435 0.9899265614697407 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 1672 0.11795223075745508 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.054559255828653E-5 2 0.0 0.0 0.0 0.0 7.054559255828653E-5 3 0.0 0.0 0.0 0.0 7.054559255828653E-5 4 0.0 0.0 0.0 0.0 7.054559255828653E-5 5 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 6 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 7 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 8 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 9 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 10 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 11 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 12 7.054559255828653E-5 0.0 0.0 0.0 7.054559255828653E-5 13 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 14 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 15 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 16 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 17 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 18 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 19 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 20 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0 7.054559255828653E-5 21 7.054559255828653E-5 7.054559255828653E-5 0.0 1.4109118511657307E-4 7.054559255828653E-5 22 7.054559255828653E-5 7.054559255828653E-5 0.0 1.4109118511657307E-4 7.054559255828653E-5 23 7.054559255828653E-5 7.054559255828653E-5 0.0 7.054559255828654E-4 1.4109118511657307E-4 24 7.054559255828653E-5 7.054559255828653E-5 0.0 0.001058183888374298 1.4109118511657307E-4 25 7.054559255828653E-5 7.054559255828653E-5 0.0 0.001199275073490871 1.4109118511657307E-4 26 7.054559255828653E-5 7.054559255828653E-5 0.0 0.0014109118511657307 1.4109118511657307E-4 27 7.054559255828653E-5 7.054559255828653E-5 0.0 0.002398550146981742 1.4109118511657307E-4 28 7.054559255828653E-5 7.054559255828653E-5 0.0 0.008818199069785817 1.4109118511657307E-4 29 7.054559255828653E-5 7.054559255828653E-5 0.0 0.021728042507952253 1.4109118511657307E-4 30 7.054559255828653E-5 7.054559255828653E-5 0.0 0.044866996867070234 1.4109118511657307E-4 31 7.054559255828653E-5 7.054559255828653E-5 0.0 0.08846417306809132 1.4109118511657307E-4 32 7.054559255828653E-5 7.054559255828653E-5 0.0 0.13036825504771352 1.4109118511657307E-4 33 7.054559255828653E-5 7.054559255828653E-5 0.0 0.17636398139571632 1.4109118511657307E-4 34 7.054559255828653E-5 7.054559255828653E-5 0.0 0.2215131606330197 1.4109118511657307E-4 35 7.054559255828653E-5 7.054559255828653E-5 0.0 0.2780907258647655 1.4109118511657307E-4 36 7.054559255828653E-5 7.054559255828653E-5 0.0 0.35216359805096636 1.4109118511657307E-4 37 7.054559255828653E-5 1.4109118511657307E-4 0.0 0.46729400510609 1.4109118511657307E-4 38 7.054559255828653E-5 1.4109118511657307E-4 0.0 0.6220710351789707 1.4109118511657307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2065 0.0 21.30556 1 TACTAAT 75 5.4788285E-5 20.530733 2 TATACAG 155 3.45608E-11 19.86845 5 TATACCG 90 1.0032563E-5 19.553078 5 ATACCGG 90 1.0032563E-5 19.553078 6 CGCAATA 440 0.0 18.501572 36 CGTTCGG 60 0.0074045607 18.336834 27 TCTAGCG 445 0.0 18.293045 28 CTATACT 135 5.5355486E-8 17.923655 4 CTAGCGG 480 0.0 16.959175 29 GTATAAT 170 2.4720066E-9 16.821993 1 TCTAGAC 170 2.4720066E-9 16.821398 3 CAATACG 460 0.0 16.739964 38 ATACGAA 475 0.0 16.673338 40 GTACTAC 160 2.1324013E-8 16.498491 1 CTAGACT 200 7.2759576E-11 16.49791 4 ACTATAC 120 6.415823E-6 16.49791 3 AACGCAG 2640 0.0 16.247942 6 GCGAAAG 625 0.0 16.191662 18 GGACCGT 165 3.1164745E-8 15.997973 6 >>END_MODULE