FastQCFastQC Report
Thu 2 Feb 2017
SRR4064223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151403
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49760.23129092968634887No Hit
TATCAACGCAGAGTACTTTTTTTTT33980.1579434443477117No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24050.11178751726199135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1900.013.500767
CGGCTAA500.001498711513.3013689
GCGTTAT1800.012.664141
ACGAACG1600.012.47032215
CGATAAC1750.011.94436410
CCGATAA1750.011.9440869
CGTTATT1750.011.9360322
CGAACGA1701.8189894E-1211.73704716
CGAACGT1302.6284397E-911.6891584
CGACCAT2350.011.32057710
GATAACG1850.011.29872211
GCATTAG1354.7457434E-911.2570121
CCGACCA2450.011.2460559
ATAACGA1900.011.00138712
TCGAACG1303.2898242E-810.9565493
GTCCTAA3650.010.9293261
CGGTCGG1752.0008883E-1110.85851210
TCGCGTA1752.0008883E-1110.858269
CGAGCCG4050.010.79148415
CGGTCCA4050.010.79148410
Produced by FastQC (version 0.11.5)