##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064223_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151403
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216192410255076	32.0	32.0	32.0	32.0	32.0
2	30.7774029319472	32.0	32.0	32.0	32.0	32.0
3	30.794825516186414	32.0	32.0	32.0	32.0	32.0
4	30.823097764575024	32.0	32.0	32.0	32.0	32.0
5	30.7356078800671	32.0	32.0	32.0	32.0	32.0
6	34.390812414038656	36.0	36.0	36.0	32.0	36.0
7	34.322852575737784	36.0	36.0	36.0	32.0	36.0
8	34.28873251547943	36.0	36.0	36.0	32.0	36.0
9	34.42952808004823	36.0	36.0	36.0	32.0	36.0
10	34.1026548721927	36.0	36.0	36.0	32.0	36.0
11	34.41053721687661	36.0	36.0	36.0	32.0	36.0
12	34.19679762461984	36.0	36.0	36.0	32.0	36.0
13	34.29850102468017	36.0	36.0	36.0	32.0	36.0
14	34.182507879741735	36.0	36.0	36.0	32.0	36.0
15	34.12072494088741	36.0	36.0	36.0	32.0	36.0
16	34.118820137370825	36.0	36.0	36.0	32.0	36.0
17	34.035454538271075	36.0	36.0	36.0	32.0	36.0
18	34.03286553007503	36.0	36.0	36.0	32.0	36.0
19	34.05963829184955	36.0	36.0	36.0	32.0	36.0
20	34.0356743948019	36.0	36.0	36.0	32.0	36.0
21	34.016096008046844	36.0	36.0	36.0	32.0	36.0
22	33.99549875127998	36.0	36.0	36.0	32.0	36.0
23	33.937528673149565	36.0	36.0	36.0	32.0	36.0
24	33.92099434647995	36.0	36.0	36.0	32.0	36.0
25	33.519020843607635	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	24.0
5	28.0
6	94.0
7	36.0
8	127.0
9	126.0
10	181.0
11	69.0
12	117.0
13	109.0
14	205.0
15	335.0
16	660.0
17	841.0
18	1127.0
19	1619.0
20	2608.0
21	3921.0
22	6120.0
23	9144.0
24	13512.0
25	19171.0
26	26941.0
27	34202.0
28	46058.0
29	59896.0
30	78143.0
31	104160.0
32	146253.0
33	206458.0
34	437679.0
35	951437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35442744261592	17.259886178113256	11.203815948735047	26.181870430535774
2	16.505430674876283	20.00308650870312	37.83176482291156	25.659717993509034
3	18.911895061484508	24.160447717833645	28.495854937934467	28.431802282747388
4	12.684880935724488	15.517796772860532	36.119409645347474	35.6779126460675
5	14.534511836162448	36.70735086026244	33.72966952460111	15.028467778974008
6	33.706287724674496	35.63296564753486	17.142157172662326	13.518589455128325
7	29.83228322441708	30.438916391741454	21.191386999230087	18.53741338461138
8	27.61403825283474	33.42608100479951	19.706012820381673	19.25386792198408
9	27.553068946963837	14.279682081583436	18.585606244155056	39.58164272729767
10	15.816998753135184	26.83219599356895	31.912158582973717	25.43864667032215
11	37.07101739229917	21.135052379663502	22.91316127988124	18.880768948156085
12	24.962268793164153	23.554133177555965	29.122259342339834	22.361338686940048
13	29.451501127880746	19.74968023999442	25.27062161345085	25.528197018673985
14	23.393896940935484	19.94971094958955	25.39987748671959	31.256514622755375
15	24.806165858123315	27.657903145017375	22.423369763129585	25.112561233729725
16	25.225989107871516	25.841246924989985	24.301102375682156	24.631661591456343
17	23.809149515762428	26.043158374033347	25.489737363383053	24.65795474682117
18	24.490769921363498	25.122280807437093	26.57309054710631	23.813858724093098
19	25.52814026992518	24.897865204862654	25.470508476153043	24.10348604905912
20	25.639403499403183	24.646212701268713	25.135156005057365	24.579227794270743
21	26.412054168029176	24.363984298286052	24.589258029359545	24.634703504325227
22	25.717500700080105	24.74799442168732	24.97304342729447	24.561461450938108
23	24.28077993910651	24.54477578960345	25.713847105698356	25.460597165591686
24	24.75116035237127	25.104406129377082	25.536450740495063	24.607982777756586
25	24.662146446932383	24.822165202619097	25.54327297897242	24.972415371476096
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	204.0
2	515.0
3	826.0
4	826.0
5	826.0
6	2276.5
7	3727.0
8	3727.0
9	3727.0
10	4098.5
11	4470.0
12	4470.0
13	4470.0
14	5339.5
15	6209.0
16	6209.0
17	6209.0
18	10528.5
19	14848.0
20	14848.0
21	14848.0
22	23833.5
23	32819.0
24	32819.0
25	32819.0
26	49188.0
27	65557.0
28	65557.0
29	65557.0
30	83602.5
31	101648.0
32	101648.0
33	101648.0
34	122329.0
35	143010.0
36	143010.0
37	143010.0
38	162017.5
39	181025.0
40	181025.0
41	181025.0
42	204483.5
43	227942.0
44	227942.0
45	227942.0
46	251985.0
47	276028.0
48	276028.0
49	276028.0
50	280780.0
51	285532.0
52	285532.0
53	285532.0
54	265711.5
55	245891.0
56	245891.0
57	245891.0
58	225584.0
59	205277.0
60	205277.0
61	205277.0
62	179222.5
63	153168.0
64	153168.0
65	153168.0
66	125622.0
67	98076.0
68	98076.0
69	98076.0
70	73806.5
71	49537.0
72	49537.0
73	49537.0
74	38082.5
75	26628.0
76	26628.0
77	26628.0
78	21422.5
79	16217.0
80	16217.0
81	16217.0
82	11217.5
83	6218.0
84	6218.0
85	6218.0
86	4756.5
87	3295.0
88	3295.0
89	3295.0
90	2394.5
91	1494.0
92	1494.0
93	1494.0
94	1015.0
95	536.0
96	536.0
97	536.0
98	878.5
99	1221.0
100	1221.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03950910173500734
2	0.0048805360966773775
3	0.002231102215623944
4	0.003997391469659567
5	0.009249777935607603
6	0.01352605718222016
7	0.023937867521798568
8	0.03555819156150661
9	0.04834054800518545
10	0.056242368352186926
11	0.05303515891722751
12	0.06163419870661146
13	0.06288919370289993
14	0.06656121609944766
15	0.05763680723695189
16	0.06553862758395336
17	0.06144827352197612
18	0.07613636310816708
19	0.0719530464538722
20	0.07739135810445555
21	0.07316156015400184
22	0.07664765736591424
23	0.08124930568563864
24	0.07553210625810228
25	0.07692654514286723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2151403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.57501620975066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07528156616226	41.29309459278239
2	13.538680504557702	14.50670054980628
3	4.249834033262205	6.830547816623181
4	1.761351559848941	3.7745775347990644
5	0.9193994739389341	2.4628420859757307
6	0.5450080200866307	1.7519288106351214
7	0.3680248439772873	1.3801855887171885
8	0.2547424802722165	1.091826600791687
9	0.2000458906045742	0.9645715648650662
>10	0.9362000570793301	8.92108544390264
>50	0.0704379278762767	2.648085721697576
>100	0.06749780917125472	7.7070101346478594
>500	0.009751827833623163	3.5594067429871243
>1k	0.0037440053289803134	3.10813681176913
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4976	0.23129092968634887	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3398	0.1579434443477117	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2405	0.11178751726199135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.648129615883217E-5	0.0
6	0.0	0.0	0.0	4.648129615883217E-5	0.0
7	0.0	0.0	0.0	4.648129615883217E-5	0.0
8	0.0	0.0	0.0	9.296259231766434E-5	0.0
9	0.0	0.0	0.0	9.296259231766434E-5	0.0
10	0.0	0.0	0.0	1.8592518463532868E-4	0.0
11	0.0	0.0	0.0	1.8592518463532868E-4	0.0
12	0.0	0.0	0.0	1.8592518463532868E-4	0.0
13	0.0	0.0	0.0	1.8592518463532868E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	190	0.0	13.50076	7
CGGCTAA	50	0.0014987115	13.301368	9
GCGTTAT	180	0.0	12.66414	1
ACGAACG	160	0.0	12.470322	15
CGATAAC	175	0.0	11.944364	10
CCGATAA	175	0.0	11.944086	9
CGTTATT	175	0.0	11.936032	2
CGAACGA	170	1.8189894E-12	11.737047	16
CGAACGT	130	2.6284397E-9	11.689158	4
CGACCAT	235	0.0	11.320577	10
GATAACG	185	0.0	11.298722	11
GCATTAG	135	4.7457434E-9	11.257012	1
CCGACCA	245	0.0	11.246055	9
ATAACGA	190	0.0	11.001387	12
TCGAACG	130	3.2898242E-8	10.956549	3
GTCCTAA	365	0.0	10.929326	1
CGGTCGG	175	2.0008883E-11	10.858512	10
TCGCGTA	175	2.0008883E-11	10.85826	9
CGAGCCG	405	0.0	10.791484	15
CGGTCCA	405	0.0	10.791484	10
>>END_MODULE