FastQCFastQC Report
Thu 2 Feb 2017
SRR4064223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151403
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36550.021.9718191
TACCGTC7250.018.5089457
GATTTCG3500.018.22846641
ACCGTCG7000.018.2271968
ATTTCGT3650.018.08166542
ATACCGT7350.017.9578276
CGTCGTA6900.017.85496910
GTATTAC2300.017.2188911
CCGTCGT7550.017.1911549
TACACGA902.217662E-417.109825
GTCCTAC9150.017.0725251
AACGCAG46750.016.9398966
TAGTACG953.3503515E-416.2089252
CGACGGT6300.016.0622797
TACGACG6350.015.9358045
GTCGTAG7600.015.92058911
GACGGTA6500.015.906498
TGTAGGA10200.015.7435432
GGTATCA18650.015.6903751
TCCTACA10950.015.6696842
Produced by FastQC (version 0.11.5)