FastQCFastQC Report
Thu 2 Feb 2017
SRR4064222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669421
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24600.36748174915337284No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17160.2563409274582064No Hit
TATCAACGCAGAGTACTTTTTTTTT16750.25021623163898354No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10830.16178159932239952No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9470.14146553514156263No Hit
GGTATCAACGCAGAGTACTTTTTTT8530.12742354960480773No Hit
GCGCAAGACGGACCAGAGCGAAAGC7850.1172655175143893No Hit
GTACATGGGGTGGTATCAACGCAAA7360.10994575909629367No Hit
GTATCTGATCGTCTTCGAACCTCCG7210.10770501672340725No Hit
GATTAAGAGGGACGGCCGGGGGCAT6720.1003852583053116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGA350.002168575516.28558710
CTAATCT350.00216965916.284374
CCAACGT456.747951E-414.77987219
TTAGGCA456.7594385E-414.7765584
CAAATCG653.362853E-614.61636313
CGGACCA1650.014.3927519
GGTTCCG801.2847886E-714.24988658
ATCGCTC707.2371568E-613.57335116
AATCGCT707.2371568E-613.57335115
AAATCGC707.24275E-613.57233714
ATCGCCA1205.0931703E-1113.46023916
TCGCGGC655.436204E-513.15570915
CAAGACG1900.012.9989264
TCGCCAG1251.0186341E-1012.9218317
AGCGAAA1850.012.83965717
CGCGGCG751.4734553E-512.66846216
GCGAAAG1800.012.66846218
TCCAACG905.380298E-712.66846218
CGCGGTC751.4757228E-512.66656710
AGGCCCG1501.8189894E-1212.66656710
Produced by FastQC (version 0.11.5)