##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064222_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669421
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20065698566373	32.0	32.0	32.0	32.0	32.0
2	30.935157397213413	32.0	32.0	32.0	32.0	32.0
3	30.93967473383715	32.0	32.0	32.0	32.0	32.0
4	30.962706577773925	32.0	32.0	32.0	32.0	32.0
5	30.93993316612416	32.0	32.0	32.0	32.0	32.0
6	34.535686809944714	36.0	36.0	36.0	32.0	36.0
7	34.47244559104062	36.0	36.0	36.0	32.0	36.0
8	34.43712551593093	36.0	36.0	36.0	32.0	36.0
9	34.511374755198894	36.0	36.0	36.0	32.0	36.0
10	34.3249927922787	36.0	36.0	36.0	32.0	36.0
11	34.49908353636949	36.0	36.0	36.0	32.0	36.0
12	34.37028417094773	36.0	36.0	36.0	32.0	36.0
13	34.417085809976086	36.0	36.0	36.0	32.0	36.0
14	34.34734046287762	36.0	36.0	36.0	32.0	36.0
15	34.31503343934534	36.0	36.0	36.0	32.0	36.0
16	34.29465612820631	36.0	36.0	36.0	32.0	36.0
17	34.23146569946267	36.0	36.0	36.0	32.0	36.0
18	34.22076092623327	36.0	36.0	36.0	32.0	36.0
19	34.23927991503105	36.0	36.0	36.0	32.0	36.0
20	34.20251978948972	36.0	36.0	36.0	32.0	36.0
21	34.174045929243334	36.0	36.0	36.0	32.0	36.0
22	34.170596679817336	36.0	36.0	36.0	32.0	36.0
23	34.132256083989	36.0	36.0	36.0	32.0	36.0
24	34.11622730688162	36.0	36.0	36.0	32.0	36.0
25	33.72619323265927	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	7.0
5	6.0
6	31.0
7	5.0
8	40.0
9	41.0
10	71.0
11	14.0
12	34.0
13	41.0
14	89.0
15	142.0
16	207.0
17	320.0
18	406.0
19	513.0
20	765.0
21	1052.0
22	1508.0
23	2354.0
24	3456.0
25	4787.0
26	6813.0
27	9272.0
28	12620.0
29	16896.0
30	22320.0
31	30408.0
32	42978.0
33	61133.0
34	135435.0
35	315654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07145600482998	17.884549572369846	11.560584798992158	25.483409623808008
2	16.28921422169107	20.608604720645353	38.0918733193905	25.01030773827308
3	18.77817779835975	24.977666900703603	28.930700168805366	27.31345513213128
4	12.240980342068033	15.804475357746279	36.793302649092695	35.16124165109299
5	14.02716960159916	37.38397343098016	33.735364554620816	14.853492412799863
6	32.74114557031999	36.39511986685113	17.5917266025823	13.272007960246576
7	29.211043773458606	31.205622893208062	21.50392072485596	18.079412608477373
8	27.52344304554115	33.15455598311354	20.240744199947695	19.081256771397616
9	27.566030178392083	14.346543354536323	19.20106174374671	38.88636472332488
10	15.708059178567051	27.1612762433747	32.20472746948549	24.92593710857276
11	36.508524888609216	21.64725330734589	23.164051231538874	18.680170572506018
12	24.508612455211196	24.333717504293894	29.444088176951833	21.713581863543073
13	29.04120277913971	20.044575057101515	25.95503031462636	24.959191849132413
14	23.115823524663437	20.53183569867527	26.15962545181668	30.19271532484461
15	24.370867064464825	28.05732598323508	23.218129312303443	24.35367763999665
16	24.642397480301778	26.306899123875695	25.04196756501422	24.008735830808302
17	23.03913948148857	26.403305271214006	26.112868315316177	24.44468693198125
18	23.647067793493488	25.509232335524494	27.62539636028758	23.21830351069444
19	24.79915325944286	25.250628627126193	26.59160016623861	23.358617947192332
20	25.111940689001482	24.794843925900125	26.01165229689586	24.081563088202536
21	26.384692548844903	24.629131578121193	25.260463774269216	23.725712098764692
22	25.12924242560151	25.04238314361831	25.92502892817739	23.90334550260279
23	23.686708837103605	24.926256553934753	26.556311885813898	24.83072272314774
24	23.998696373016713	25.60759930812091	26.38484204735216	24.00886227151022
25	23.949439756019167	25.32123395698941	26.45861519946779	24.27071108752364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	336.0
1	336.0
2	438.0
3	540.0
4	540.0
5	540.0
6	1413.5
7	2287.0
8	2287.0
9	2287.0
10	2256.5
11	2226.0
12	2226.0
13	2226.0
14	2360.0
15	2494.0
16	2494.0
17	2494.0
18	4204.5
19	5915.0
20	5915.0
21	5915.0
22	9279.0
23	12643.0
24	12643.0
25	12643.0
26	18239.0
27	23835.0
28	23835.0
29	23835.0
30	29552.0
31	35269.0
32	35269.0
33	35269.0
34	41390.0
35	47511.0
36	47511.0
37	47511.0
38	52507.5
39	57504.0
40	57504.0
41	57504.0
42	64700.0
43	71896.0
44	71896.0
45	71896.0
46	79786.5
47	87677.0
48	87677.0
49	87677.0
50	88037.0
51	88397.0
52	88397.0
53	88397.0
54	80053.0
55	71709.0
56	71709.0
57	71709.0
58	65236.5
59	58764.0
60	58764.0
61	58764.0
62	51035.0
63	43306.0
64	43306.0
65	43306.0
66	35403.5
67	27501.0
68	27501.0
69	27501.0
70	20577.5
71	13654.0
72	13654.0
73	13654.0
74	10510.0
75	7366.0
76	7366.0
77	7366.0
78	6049.0
79	4732.0
80	4732.0
81	4732.0
82	3267.0
83	1802.0
84	1802.0
85	1802.0
86	1457.5
87	1113.0
88	1113.0
89	1113.0
90	786.0
91	459.0
92	459.0
93	459.0
94	303.5
95	148.0
96	148.0
97	148.0
98	242.5
99	337.0
100	337.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040034597062237366
2	0.0031370393220409875
3	0.001643211073450041
4	0.003286422146900082
5	0.010456797740136626
6	0.014191368361613993
7	0.02345310350287786
8	0.03674817491533728
9	0.04974448067807852
10	0.059006215819342386
11	0.05676547344645597
12	0.0669235055368744
13	0.06528029446342436
14	0.06796918531088807
15	0.060500044067933335
16	0.06752103683631078
17	0.0640852318645516
18	0.07797783457644741
19	0.07513956090412462
20	0.08066672542391112
21	0.07738030327701102
22	0.0781272174013065
23	0.08186178802278386
24	0.07738030327701102
25	0.07708153762729283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	669421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.432981501218784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.76819559215018	48.597276560163
2	11.912784693576365	14.160246246426528
3	3.0634079574402056	5.462024053956905
4	1.1348457435080612	2.697890643226059
5	0.5469979568929915	1.6254859726612816
6	0.29174236917504737	1.04034712981814
7	0.2055143506316915	0.8550031419529817
8	0.14544595923859882	0.691542960388373
9	0.10972540326694169	0.5869177076320125
>10	0.6303395235162832	7.251929046620338
>50	0.09903732748978275	4.132119703365901
>100	0.08514168266321605	9.663075921613922
>500	0.00581085668025068	2.1949660288681128
>1k	0.001010583770478379	1.0411748833063001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2460	0.36748174915337284	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1716	0.2563409274582064	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1675	0.25021623163898354	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1083	0.16178159932239952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	947	0.14146553514156263	No Hit
GGTATCAACGCAGAGTACTTTTTTT	853	0.12742354960480773	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	785	0.1172655175143893	No Hit
GTACATGGGGTGGTATCAACGCAAA	736	0.10994575909629367	No Hit
GTATCTGATCGTCTTCGAACCTCCG	721	0.10770501672340725	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	672	0.1003852583053116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCGA	35	0.0021685755	16.285587	10
CTAATCT	35	0.002169659	16.28437	4
CCAACGT	45	6.747951E-4	14.779872	19
TTAGGCA	45	6.7594385E-4	14.776558	4
CAAATCG	65	3.362853E-6	14.616363	13
CGGACCA	165	0.0	14.392751	9
GGTTCCG	80	1.2847886E-7	14.2498865	8
ATCGCTC	70	7.2371568E-6	13.573351	16
AATCGCT	70	7.2371568E-6	13.573351	15
AAATCGC	70	7.24275E-6	13.572337	14
ATCGCCA	120	5.0931703E-11	13.460239	16
TCGCGGC	65	5.436204E-5	13.155709	15
CAAGACG	190	0.0	12.998926	4
TCGCCAG	125	1.0186341E-10	12.92183	17
AGCGAAA	185	0.0	12.839657	17
CGCGGCG	75	1.4734553E-5	12.668462	16
GCGAAAG	180	0.0	12.668462	18
TCCAACG	90	5.380298E-7	12.668462	18
CGCGGTC	75	1.4757228E-5	12.666567	10
AGGCCCG	150	1.8189894E-12	12.666567	10
>>END_MODULE