FastQCFastQC Report
Thu 2 Feb 2017
SRR4064222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669421
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.1172655175143893No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7440.11114082169516643No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7400.11054329039573005No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7040.10516550870080263No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6710.10023587548045251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCG300.005744729729.331745
GGACGTG1950.025.9473086
TAGGACG2200.023.99874
AGGACGT2200.022.9987535
GACGTGA1451.2732926E-1121.2402277
TTAGGAC1601.8189894E-1220.6238783
GTATCAA20100.019.154621
AATCGCT1051.789902E-618.85752715
ATTATAC1051.7911825E-618.856123
GATTTCG951.595095E-518.52669341
GTCATAA600.007407861818.3337081
GCACCGT851.43022E-418.1166636
CGTGAAA1509.571522E-917.6003599
CGGTCCA2400.017.41702310
GTAAACG1651.6552804E-917.33239227
CGCAATA2550.017.25654236
CAAATCG1154.266305E-617.21774313
AACGCAG22500.017.012416
GTATTAG1954.9112714E-1116.9234221
AATATGG4050.016.83984814
Produced by FastQC (version 0.11.5)