##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669421
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243983980185863	32.0	32.0	32.0	32.0	32.0
2	31.338047357343136	32.0	32.0	32.0	32.0	32.0
3	31.426476014346726	32.0	32.0	32.0	32.0	32.0
4	31.52068728050061	32.0	32.0	32.0	32.0	32.0
5	31.46424746161235	32.0	32.0	32.0	32.0	32.0
6	35.014697776137886	36.0	36.0	36.0	36.0	36.0
7	35.040521585071275	36.0	36.0	36.0	36.0	36.0
8	34.9864823481785	36.0	36.0	36.0	36.0	36.0
9	35.09756789822847	36.0	36.0	36.0	36.0	36.0
10	34.95852535250612	36.0	36.0	36.0	36.0	36.0
11	35.09815198507367	36.0	36.0	36.0	36.0	36.0
12	34.99632368868022	36.0	36.0	36.0	36.0	36.0
13	35.045908329735695	36.0	36.0	36.0	36.0	36.0
14	34.98346630894459	36.0	36.0	36.0	36.0	36.0
15	34.96442896174455	36.0	36.0	36.0	36.0	36.0
16	34.959668131116295	36.0	36.0	36.0	36.0	36.0
17	34.932919642497026	36.0	36.0	36.0	32.0	36.0
18	34.92877576293543	36.0	36.0	36.0	32.0	36.0
19	34.92526377272299	36.0	36.0	36.0	32.0	36.0
20	34.91732108792524	36.0	36.0	36.0	32.0	36.0
21	34.91510872828908	36.0	36.0	36.0	32.0	36.0
22	34.899552000908244	36.0	36.0	36.0	32.0	36.0
23	34.85233059614204	36.0	36.0	36.0	32.0	36.0
24	34.81449192660523	36.0	36.0	36.0	32.0	36.0
25	34.78401633650573	36.0	36.0	36.0	32.0	36.0
26	34.725528479088645	36.0	36.0	36.0	32.0	36.0
27	34.72006256152705	36.0	36.0	36.0	32.0	36.0
28	34.688728020184605	36.0	36.0	36.0	32.0	36.0
29	34.66334489058455	36.0	36.0	36.0	32.0	36.0
30	34.636760723072626	36.0	36.0	36.0	32.0	36.0
31	34.63178478117657	36.0	36.0	36.0	32.0	36.0
32	34.57900036001261	36.0	36.0	36.0	32.0	36.0
33	34.55384578613459	36.0	36.0	36.0	32.0	36.0
34	34.52215123218423	36.0	36.0	36.0	32.0	36.0
35	34.472869838263215	36.0	36.0	36.0	32.0	36.0
36	34.447851202755814	36.0	36.0	36.0	32.0	36.0
37	34.44099453109478	36.0	36.0	36.0	32.0	36.0
38	34.39100655641219	36.0	36.0	36.0	32.0	36.0
39	34.37832694223815	36.0	36.0	36.0	32.0	36.0
40	34.34520727613863	36.0	36.0	36.0	32.0	36.0
41	34.325833817582655	36.0	36.0	36.0	32.0	36.0
42	34.25448260511696	36.0	36.0	36.0	32.0	36.0
43	34.247973995437846	36.0	36.0	36.0	32.0	36.0
44	34.17038605003428	36.0	36.0	36.0	32.0	36.0
45	34.133476541668095	36.0	36.0	36.0	32.0	36.0
46	34.08541709925443	36.0	36.0	36.0	32.0	36.0
47	34.04477302026677	36.0	36.0	36.0	32.0	36.0
48	33.991458290074554	36.0	36.0	36.0	32.0	36.0
49	33.95395871955018	36.0	36.0	36.0	32.0	36.0
50	33.34365518858835	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	6.0
20	7.0
21	29.0
22	85.0
23	257.0
24	596.0
25	1425.0
26	2889.0
27	5212.0
28	8703.0
29	13320.0
30	19085.0
31	26862.0
32	38248.0
33	59883.0
34	120563.0
35	372249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.058850596692935	18.849760067886148	12.150484943736965	25.940904391683944
2	15.454997677097074	21.4794576208395	37.583523671949344	25.482021030114083
3	17.93913826766196	25.572922190735547	29.27897176506792	27.208967776534575
4	11.857560488840356	16.6257108755178	36.894121935224625	34.62260670041722
5	13.640295120708792	37.85017201432282	33.47728858222255	15.032244282745836
6	32.75815966335087	36.958655315563746	17.212038463089748	13.071146557995641
7	28.93336181565861	31.29316229995772	21.601354005924524	18.172121878459148
8	27.46552617859314	33.42844039849363	19.982492332926512	19.12354108998672
9	27.45796335910704	14.110209496038337	19.087981165672765	39.34384597918185
10	15.524888371683081	27.48797820166744	32.13610811925146	24.851025307398018
11	36.72427366335983	21.59702190400361	23.049620492933446	18.62908393970312
12	24.46771164932083	24.412440004122963	29.467405414529868	21.652442932026332
13	29.104877826526664	20.015625536847732	25.878189529097707	25.001307107527893
14	23.128942772933627	20.350272847729606	26.184717838251263	30.336066541085504
15	24.47426250349197	28.032710231508247	23.16994650377282	24.32308076122696
16	24.6820353323952	26.313014069021563	24.911937903898036	24.093012694685203
17	22.950430297227005	26.432543944692505	26.17381289203655	24.44321286604394
18	23.89075286293467	25.344851796635286	27.481483915879167	23.28291142455088
19	24.871493424914963	25.129925711921196	26.58805146537082	23.410529397793017
20	25.34100568106278	24.67236416165111	25.98604457311362	24.000585584172494
21	26.44807975847785	24.55211294536622	25.45244323079198	23.547364065363947
22	25.188633162090824	24.91615888954783	26.118840012488402	23.77636793587294
23	23.76896245229043	24.806883622267204	26.45727986376164	24.96687406168072
24	24.087867108840488	25.505213468375608	26.477248961787815	23.929670460996086
25	24.16832859031528	24.97441757979101	26.644856252941047	24.212397576952664
26	23.69464052287582	26.03264239028945	26.891951447245567	23.380765639589168
27	24.372622778894907	25.316639128499162	26.084221974561306	24.22651611804462
28	23.463935021340358	25.436629523341438	26.910959098521186	24.188476356797022
29	23.663543974389043	25.31561799280252	26.728971124931096	24.291866907877342
30	23.389758092105165	25.70336740928641	26.977368846422173	23.929505652186254
31	24.285812460223315	25.388803069191262	25.8909229983178	24.43446147226762
32	23.944038602309565	25.759870927262135	25.71102048133375	24.58506998909455
33	23.683408527293913	25.214526875616244	26.35931757745974	24.742747019630105
34	24.32439699911264	25.198460715683552	26.704431716666516	23.772710568537292
35	24.956415868059963	25.241897534340712	26.455680129071773	23.34600646852755
36	23.59729835113368	25.98028889894811	26.155528564961926	24.26688418495628
37	24.673729877021263	25.869157314784935	25.670168393627574	23.78694441456623
38	23.60424292223799	25.700754463285275	26.389183536266525	24.305819078210202
39	24.33505632077445	25.547222803191012	25.777435836146882	24.340285039887657
40	24.674222698271137	25.599978488166254	26.405477151892963	23.320321661669645
41	23.581478172795094	25.91269118038106	26.728653080248705	23.77717756657514
42	24.845944359210698	26.287232914202395	25.92840464833486	22.93841807825205
43	24.025371633784427	25.357890318176185	26.320231726791842	24.29650632124755
44	23.599315810309154	26.048953921766344	25.84922431114198	24.502505956782517
45	23.823103663081913	26.325310833884814	25.79110827610624	24.060477226927034
46	23.67950882120076	25.794356224137672	25.88488370355985	24.641251251101718
47	23.972668224271143	25.613979002216897	26.28278289343954	24.13056988007242
48	24.58941210565785	26.17678957165009	25.269866120112454	23.9639322025796
49	23.64759185978247	26.46242924813681	25.436611260245854	24.453367631834865
50	23.958225962824034	26.56589150760893	25.296566683248408	24.17931584631863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	123.5
2	43.0
3	140.5
4	238.0
5	245.5
6	253.0
7	238.5
8	224.0
9	308.5
10	393.0
11	567.0
12	741.0
13	1299.0
14	1857.0
15	2412.0
16	2967.0
17	3093.0
18	3219.0
19	3211.5
20	3204.0
21	3532.5
22	3861.0
23	4306.0
24	4751.0
25	5363.5
26	5976.0
27	7236.5
28	8497.0
29	9860.5
30	11224.0
31	12630.0
32	14036.0
33	16004.5
34	17973.0
35	20458.0
36	22943.0
37	25940.5
38	28938.0
39	30690.0
40	32442.0
41	34690.0
42	36938.0
43	36847.5
44	36757.0
45	40130.5
46	43504.0
47	45587.5
48	47671.0
49	49007.5
50	50344.0
51	48270.5
52	46197.0
53	45240.0
54	44283.0
55	44416.5
56	44550.0
57	42826.0
58	41102.0
59	36947.0
60	32792.0
61	28513.5
62	24235.0
63	20941.0
64	17647.0
65	14840.5
66	12034.0
67	10385.5
68	8737.0
69	8038.0
70	7339.0
71	5648.0
72	3957.0
73	3391.0
74	2825.0
75	2153.5
76	1482.0
77	1305.0
78	1128.0
79	953.0
80	778.0
81	623.0
82	468.0
83	414.5
84	361.0
85	258.5
86	156.0
87	107.0
88	58.0
89	46.0
90	34.0
91	25.5
92	17.0
93	14.0
94	11.0
95	13.0
96	15.0
97	12.5
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009709883615841153
2	0.0
3	2.987656497181893E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	7.469141242954733E-4
10	0.001792593898309136
11	0.0
12	0.0
13	5.975312994363786E-4
14	0.0
15	0.00418271909605465
16	0.0010456797740136625
17	0.0
18	4.48148474577284E-4
19	0.0
20	5.975312994363786E-4
21	0.0
22	0.0
23	8.96296949154568E-4
24	1.4938282485909464E-4
25	0.0023901251977455143
26	0.0068716099435183545
27	0.007020992768377449
28	0.004780250395491029
29	0.0029876564971818933
30	0.005079016045209218
31	0.008813586666686584
32	0.0046308675706319335
33	0.006124695819222881
34	0.0028382736723227982
35	0.0038839534463364607
36	0.0077679068926729215
37	0.005527164519786502
38	0.01060618056499572
39	0.006124695819222881
40	0.003286422146900082
41	0.004033336271195556
42	0.0029876564971818933
43	0.001792593898309136
44	0.0023901251977455143
45	0.001792593898309136
46	0.001643211073450041
47	0.002539508022604609
48	0.0022407423728864197
49	5.975312994363786E-4
50	0.0014938282485909467
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	669421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.93338598895678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.95670200045488	53.037028493659456
2	11.459358017340334	14.652711186165321
3	2.7737741387593378	5.320103179784608
4	0.9930670666579902	2.5396056034226544
5	0.46078504352716687	1.4729774022880304
6	0.284575332856761	1.0916318759080295
7	0.18341544897628292	0.8208459487016954
8	0.12410518094964429	0.6347571549506348
9	0.087820245838441	0.5053181107350712
>10	0.528019344849947	6.6620770678094186
>50	0.07830428636696823	3.5344458147471336
>100	0.06631153672851475	8.195806057566994
>500	0.0037623566937326474	1.5326921042609454
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	785	0.1172655175143893	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	744	0.11114082169516643	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	740	0.11054329039573005	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	704	0.10516550870080263	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	671	0.10023587548045251	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.4938282485909464E-4	0.0
21	0.0	0.0	0.0	4.4814847457728393E-4	0.0
22	0.0	0.0	0.0	7.469141242954732E-4	0.0
23	0.0	0.0	0.0	0.0011950625988727571	0.0
24	0.0	0.0	0.0	0.0019419767231682304	0.0
25	0.0	0.0	0.0	0.0028382736723227982	0.0
26	0.0	0.0	0.0	0.0038839534463364607	0.0
27	0.0	0.0	0.0	0.005079016045209218	0.0
28	0.0	0.0	0.0	0.008216055367250206	0.0
29	0.0	0.0	0.0	0.016581493559359506	0.0
30	0.0	0.0	0.0	0.03151977604526897	0.0
31	0.0	0.0	0.0	0.06363708338997433	0.0
32	0.0	0.0	0.0	0.09665068768383424	0.0
33	0.0	0.0	0.0	0.13041120610198964	0.0
34	0.0	0.0	0.0	0.1677569123167633	0.0
35	0.0	0.0	0.0	0.21122731435075984	0.0
36	0.0	0.0	0.0	0.27381871796672047	0.0
37	0.0	0.0	0.0	0.3628508815827409	0.0
38	0.0	0.0	0.0	0.48713739186550764	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTGCG	30	0.0057447297	29.33174	5
GGACGTG	195	0.0	25.947308	6
TAGGACG	220	0.0	23.9987	4
AGGACGT	220	0.0	22.998753	5
GACGTGA	145	1.2732926E-11	21.240227	7
TTAGGAC	160	1.8189894E-12	20.623878	3
GTATCAA	2010	0.0	19.15462	1
AATCGCT	105	1.789902E-6	18.857527	15
ATTATAC	105	1.7911825E-6	18.85612	3
GATTTCG	95	1.595095E-5	18.526693	41
GTCATAA	60	0.0074078618	18.333708	1
GCACCGT	85	1.43022E-4	18.116663	6
CGTGAAA	150	9.571522E-9	17.600359	9
CGGTCCA	240	0.0	17.417023	10
GTAAACG	165	1.6552804E-9	17.332392	27
CGCAATA	255	0.0	17.256542	36
CAAATCG	115	4.266305E-6	17.217743	13
AACGCAG	2250	0.0	17.01241	6
GTATTAG	195	4.9112714E-11	16.923422	1
AATATGG	405	0.0	16.839848	14
>>END_MODULE