FastQCFastQC Report
Thu 2 Feb 2017
SRR4064221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558028
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31430.56323338613833No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25500.45696631710236757No Hit
TATCAACGCAGAGTACTTTTTTTTT23620.42327625137089897No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17250.3091242733339546No Hit
GTCCTAAAGTGTGTATTTCTCATTT16820.3014185668102676No Hit
GTCCTACAGTGGACATTTCTAAATT16190.29012881074067964No Hit
CTGTAGGACGTGGAATATGGCAAGA15190.2722085630111751No Hit
CTTTAGGACGTGAAATATGGCGAGG14440.2587683772140466No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13990.25070426573576954No Hit
GTACATGGAAGCAGTGGTATCAACG12500.22400309661880766No Hit
GTACATGGGGTGGTATCAACGCAAA12430.22274867927774233No Hit
GTCCTACAGTGTGCATTTCTCATTT10710.1919258531829944No Hit
TCCATGTACTCTGCGTTGATACCAC10670.19120904327381422No Hit
GGTATCAACGCAGAGTACTTTTTTT10570.18941701850086373No Hit
CTGTAGGACCTGGAATATGGCGAGA8080.14479560165439725No Hit
CTGAAGGACCTGGAATATGGCGAGA8040.1440787917452171No Hit
GTATCAACGCAGAGTACATGGGGTG7520.1347602629258747No Hit
GTCCTTCAGTGTGCATTTCTCATTT6730.12060326721956603No Hit
ATTTAGAAATGTCCACTGTAGGACG6640.11899044492391063No Hit
GTACATGGGTGGTATCAACGCAAAA6560.11755682510555027No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6480.11612320528718988No Hit
GTACATGGGAGTGGTATCAACGCAA6070.108775903718093No Hit
TTGTAGAACAGTGTATATCAATGAG5990.10734228389973263No Hit
GATATACACTGTTCTACAAATCCCG5910.10590866408137227No Hit
TTTCTAAATTTTCCACCTTTTTCAG5670.10160780462629115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG250.006016323319.00601415
ATAACGA250.006019443719.00430912
CGCATCG453.525456E-516.89271713
CCGTCGT402.7580257E-416.6242949
CGTCGTA402.7580257E-416.62429410
GACGGAC601.4647121E-615.8312417
TGGGTAA456.754883E-414.7771510
GGTCGGC400.00526555614.25323111
TTAGTCT400.00527811114.24811654
TAATCAG400.005284398314.2455615
TAAACTG400.005284398314.2455615
TATAGGA759.69425E-713.9264942
CGCCAGT551.954331E-413.82007418
GTCTTAG551.963277E-413.8126371
GAAATGT2500.013.6769656
GCGAAAG707.2323273E-613.57328818
CGGACCA707.2457497E-613.5708529
GATATAC2850.013.3279841
ACCGTCG500.001496910913.3006288
GTTGTAG500.001501980213.2946631
Produced by FastQC (version 0.11.5)