##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064221_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558028
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21301619273585	32.0	32.0	32.0	32.0	32.0
2	31.019285770606494	32.0	32.0	32.0	32.0	32.0
3	31.03283526991477	32.0	32.0	32.0	32.0	32.0
4	31.02838208835399	32.0	32.0	32.0	32.0	32.0
5	31.05317116703821	32.0	32.0	32.0	32.0	32.0
6	34.61428279584537	36.0	36.0	36.0	32.0	36.0
7	34.53210949988173	36.0	36.0	36.0	32.0	36.0
8	34.491930512447404	36.0	36.0	36.0	32.0	36.0
9	34.55024299855921	36.0	36.0	36.0	32.0	36.0
10	34.389964302866524	36.0	36.0	36.0	32.0	36.0
11	34.56685327617969	36.0	36.0	36.0	32.0	36.0
12	34.44122517149677	36.0	36.0	36.0	32.0	36.0
13	34.492342678145185	36.0	36.0	36.0	32.0	36.0
14	34.43410187302429	36.0	36.0	36.0	32.0	36.0
15	34.40143863748772	36.0	36.0	36.0	32.0	36.0
16	34.40630756879583	36.0	36.0	36.0	32.0	36.0
17	34.35078168120596	36.0	36.0	36.0	32.0	36.0
18	34.351761918756765	36.0	36.0	36.0	32.0	36.0
19	34.31653608779488	36.0	36.0	36.0	32.0	36.0
20	34.25191746650706	36.0	36.0	36.0	32.0	36.0
21	34.20975829169863	36.0	36.0	36.0	32.0	36.0
22	34.1910441769947	36.0	36.0	36.0	32.0	36.0
23	34.1624112051725	36.0	36.0	36.0	32.0	36.0
24	34.143598529106065	36.0	36.0	36.0	32.0	36.0
25	33.599077465646886	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	2.0
6	24.0
7	3.0
8	28.0
9	31.0
10	54.0
11	15.0
12	37.0
13	34.0
14	101.0
15	171.0
16	290.0
17	424.0
18	480.0
19	619.0
20	801.0
21	950.0
22	1214.0
23	1713.0
24	2375.0
25	3311.0
26	4570.0
27	6216.0
28	8519.0
29	12140.0
30	17061.0
31	24357.0
32	36919.0
33	56642.0
34	130265.0
35	248660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92437700888976	17.910736725865988	12.01416911512944	25.150717150114822
2	14.311955871125909	20.463188303984573	41.59770036666535	23.627155458224163
3	19.461419083835406	25.600961969241354	28.73632222385497	26.201296723068268
4	11.997706154852468	16.094011809718374	36.86710930709748	35.041172728331674
5	12.859728668972561	38.505170343554546	34.35419989605549	14.280901091417409
6	32.00886800331923	36.25253377924263	18.163097420787473	13.57550079665066
7	28.260483823268466	31.267665714936076	21.668450041313353	18.803400420482102
8	26.323422120164274	36.065952858548755	20.07500183740161	17.53562318388536
9	27.349347045029155	14.543576945418558	19.518907367167948	38.588168642384346
10	15.683641305712042	28.114118916903042	33.189656718077075	23.012583059307843
11	35.42014787828369	21.1504672293978	24.731603066573914	18.697781825744592
12	25.403528023725762	23.659239814525932	31.19654441587503	19.740687745873274
13	30.29614594278624	21.148004834157554	25.893583982729	22.662265240327205
14	21.997805240168407	21.61479813230242	26.88383552211615	29.503561105413024
15	22.952862496189912	31.214207591486918	23.914438886199417	21.91849102612375
16	22.17934694902186	26.488192364933923	28.855815955100507	22.476644730943715
17	21.24312383903149	27.81339874203011	28.4294740767835	22.5140033421549
18	21.81494738503253	25.62047643982813	31.680522778299824	20.884053396839516
19	24.388831207489854	25.364872207308743	27.416118394508494	22.830178190692912
20	24.704112301037938	25.73398081930407	28.404143145088984	21.157763734569006
21	24.146489299509717	25.2070339539271	26.28264214418673	24.363834602376453
22	24.058007918973974	27.425723754052733	26.767781826528562	21.748486500444724
23	21.781594044615172	27.389605335600137	28.17186830292694	22.656932316857752
24	23.7536157293417	26.293099583417767	28.481277112366016	21.472007574874514
25	22.841659209632038	26.824343347577912	28.417309406264625	21.91668803652542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	213.0
1	213.0
2	453.0
3	693.0
4	693.0
5	693.0
6	1894.5
7	3096.0
8	3096.0
9	3096.0
10	2922.5
11	2749.0
12	2749.0
13	2749.0
14	2372.0
15	1995.0
16	1995.0
17	1995.0
18	3536.0
19	5077.0
20	5077.0
21	5077.0
22	8624.5
23	12172.0
24	12172.0
25	12172.0
26	18865.5
27	25559.0
28	25559.0
29	25559.0
30	33546.5
31	41534.0
32	41534.0
33	41534.0
34	44371.5
35	47209.0
36	47209.0
37	47209.0
38	49942.5
39	52676.0
40	52676.0
41	52676.0
42	57395.0
43	62114.0
44	62114.0
45	62114.0
46	69605.0
47	77096.0
48	77096.0
49	77096.0
50	73404.5
51	69713.0
52	69713.0
53	69713.0
54	60380.5
55	51048.0
56	51048.0
57	51048.0
58	45392.5
59	39737.0
60	39737.0
61	39737.0
62	34247.0
63	28757.0
64	28757.0
65	28757.0
66	23318.5
67	17880.0
68	17880.0
69	17880.0
70	13629.0
71	9378.0
72	9378.0
73	9378.0
74	7040.0
75	4702.0
76	4702.0
77	4702.0
78	3683.5
79	2665.0
80	2665.0
81	2665.0
82	1792.0
83	919.0
84	919.0
85	919.0
86	704.5
87	490.0
88	490.0
89	490.0
90	346.0
91	202.0
92	202.0
93	202.0
94	139.0
95	76.0
96	76.0
97	76.0
98	177.0
99	278.0
100	278.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03494448307253399
2	0.0046592644096711995
3	0.0010752148637702768
4	0.0023296322048355998
5	0.006809694137211752
6	0.012006565978768091
7	0.017382640297619472
8	0.03136043352663308
9	0.04587583418753181
10	0.05268552832474356
11	0.048743073824252546
12	0.05770319768900486
13	0.05913681750736522
14	0.060212032371135496
15	0.05340233823392374
16	0.06057043732572559
17	0.05447755309769402
18	0.07024737109965809
19	0.06576730916728193
20	0.06881375128129771
21	0.06988896614506798
22	0.06809694137211753
23	0.07436902807744414
24	0.07078497853154322
25	0.06970976366777294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	558028.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.81929135982473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.97626732408176	49.03762219650126
2	11.857492298894233	14.186135732488642
3	2.9975913512680465	5.379413712575819
4	1.0839698055476268	2.5936922249322416
5	0.5291412864100009	1.5826428391136154
6	0.29540869105434764	1.060268313624268
7	0.21102127759016032	0.8836200301101778
8	0.13089900673126964	0.6264228657895582
9	0.11438515678749844	0.6158195118999542
>10	0.6477337901361048	7.79284293166052
>50	0.08898029695127174	3.725558325478129
>100	0.05752261246941033	6.384059653082861
>500	0.005392744919007164	1.9864386388964694
>1k	0.004194357159227794	4.1454630238464665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3143	0.56323338613833	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2550	0.45696631710236757	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2362	0.42327625137089897	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1725	0.3091242733339546	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1682	0.3014185668102676	No Hit
GTCCTACAGTGGACATTTCTAAATT	1619	0.29012881074067964	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1519	0.2722085630111751	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1444	0.2587683772140466	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1399	0.25070426573576954	No Hit
GTACATGGAAGCAGTGGTATCAACG	1250	0.22400309661880766	No Hit
GTACATGGGGTGGTATCAACGCAAA	1243	0.22274867927774233	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1071	0.1919258531829944	No Hit
TCCATGTACTCTGCGTTGATACCAC	1067	0.19120904327381422	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1057	0.18941701850086373	No Hit
CTGTAGGACCTGGAATATGGCGAGA	808	0.14479560165439725	No Hit
CTGAAGGACCTGGAATATGGCGAGA	804	0.1440787917452171	No Hit
GTATCAACGCAGAGTACATGGGGTG	752	0.1347602629258747	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	673	0.12060326721956603	No Hit
ATTTAGAAATGTCCACTGTAGGACG	664	0.11899044492391063	No Hit
GTACATGGGTGGTATCAACGCAAAA	656	0.11755682510555027	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	648	0.11612320528718988	No Hit
GTACATGGGAGTGGTATCAACGCAA	607	0.108775903718093	No Hit
TTGTAGAACAGTGTATATCAATGAG	599	0.10734228389973263	No Hit
GATATACACTGTTCTACAAATCCCG	591	0.10590866408137227	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	567	0.10160780462629115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.7920247729504612E-4
13	0.0	0.0	0.0	0.0	3.5840495459009224E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	25	0.0060163233	19.006014	15
ATAACGA	25	0.0060194437	19.004309	12
CGCATCG	45	3.525456E-5	16.892717	13
CCGTCGT	40	2.7580257E-4	16.624294	9
CGTCGTA	40	2.7580257E-4	16.624294	10
GACGGAC	60	1.4647121E-6	15.831241	7
TGGGTAA	45	6.754883E-4	14.77715	10
GGTCGGC	40	0.005265556	14.253231	11
TTAGTCT	40	0.005278111	14.2481165	4
TAATCAG	40	0.0052843983	14.245561	5
TAAACTG	40	0.0052843983	14.245561	5
TATAGGA	75	9.69425E-7	13.926494	2
CGCCAGT	55	1.954331E-4	13.820074	18
GTCTTAG	55	1.963277E-4	13.812637	1
GAAATGT	250	0.0	13.676965	6
GCGAAAG	70	7.2323273E-6	13.573288	18
CGGACCA	70	7.2457497E-6	13.570852	9
GATATAC	285	0.0	13.327984	1
ACCGTCG	50	0.0014969109	13.300628	8
GTTGTAG	50	0.0015019802	13.294663	1
>>END_MODULE