FastQCFastQC Report
Thu 2 Feb 2017
SRR4064221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558028
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.16020701470177123No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8670.155368547814805No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA7850.1406739446766112No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6350.11379357308235429No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA5970.10698387894514252No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5970.10698387894514252No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA5800.10393743683112676No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5790.10375823435383172No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC5590.10017418480793079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA10850.022.5043452
TAATACC500.00257907921.9994624
CATGCGG500.00257907921.99946210
GCGGCGT703.211959E-521.99946240
ACGACGG703.211959E-521.9994626
GTCCTAG703.214182E-521.997491
GCGTTAT703.214182E-521.9974943
TATTATG703.214182E-521.997492
TAGGACC4700.021.5313874
GATATAC4200.021.4737421
CTGTAGG11050.021.1016651
ATACACT4300.020.976234
GCTAAGA956.860191E-720.8397271
GTAGGAC11050.020.7053763
TCGTTTA755.4593584E-520.5365130
TACGACG755.4668988E-520.5328315
CGAATGT2800.020.43356533
AGTGTAT2950.020.13510110
AACGAAT2750.020.00309631
GTATAGG550.004483597419.9977191
Produced by FastQC (version 0.11.5)