##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558028
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11812668898335	32.0	32.0	32.0	32.0	32.0
2	31.28719347416259	32.0	32.0	32.0	32.0	32.0
3	31.401986997068246	32.0	32.0	32.0	32.0	32.0
4	31.501017870071035	32.0	32.0	32.0	32.0	32.0
5	31.40820173898084	32.0	32.0	32.0	32.0	32.0
6	34.998503659314586	36.0	36.0	36.0	36.0	36.0
7	35.04317704487947	36.0	36.0	36.0	36.0	36.0
8	34.97068785078885	36.0	36.0	36.0	36.0	36.0
9	35.115101751166605	36.0	36.0	36.0	36.0	36.0
10	34.95012795056879	36.0	36.0	36.0	36.0	36.0
11	35.10558430759747	36.0	36.0	36.0	36.0	36.0
12	35.01336671278144	36.0	36.0	36.0	36.0	36.0
13	35.05148666375164	36.0	36.0	36.0	36.0	36.0
14	34.990633086511785	36.0	36.0	36.0	36.0	36.0
15	34.94770692510053	36.0	36.0	36.0	36.0	36.0
16	34.97739181546446	36.0	36.0	36.0	36.0	36.0
17	34.945167625997264	36.0	36.0	36.0	36.0	36.0
18	34.943803895145045	36.0	36.0	36.0	36.0	36.0
19	34.92274760406288	36.0	36.0	36.0	36.0	36.0
20	34.89973262990387	36.0	36.0	36.0	32.0	36.0
21	34.886849763811135	36.0	36.0	36.0	32.0	36.0
22	34.87409771552682	36.0	36.0	36.0	32.0	36.0
23	34.83384704710158	36.0	36.0	36.0	32.0	36.0
24	34.81507917165447	36.0	36.0	36.0	32.0	36.0
25	34.784313690352455	36.0	36.0	36.0	32.0	36.0
26	34.713792139462534	36.0	36.0	36.0	32.0	36.0
27	34.68962130932498	36.0	36.0	36.0	32.0	36.0
28	34.65151390252819	36.0	36.0	36.0	32.0	36.0
29	34.616544331108834	36.0	36.0	36.0	32.0	36.0
30	34.56598952023913	36.0	36.0	36.0	32.0	36.0
31	34.55714946203416	36.0	36.0	36.0	32.0	36.0
32	34.50236905674984	36.0	36.0	36.0	32.0	36.0
33	34.46277247736673	36.0	36.0	36.0	32.0	36.0
34	34.4270789279391	36.0	36.0	36.0	32.0	36.0
35	34.367203796225276	36.0	36.0	36.0	32.0	36.0
36	34.303914857318986	36.0	36.0	36.0	32.0	36.0
37	34.249661307317915	36.0	36.0	36.0	32.0	36.0
38	34.18758377715814	36.0	36.0	36.0	32.0	36.0
39	34.09560093758736	36.0	36.0	36.0	32.0	36.0
40	34.0815532553922	36.0	36.0	36.0	32.0	36.0
41	34.05185402883009	36.0	36.0	36.0	32.0	36.0
42	33.97196556445196	36.0	36.0	36.0	32.0	36.0
43	33.95050427577111	36.0	36.0	36.0	32.0	36.0
44	33.83639351430394	36.0	36.0	36.0	32.0	36.0
45	33.75115227192901	36.0	36.0	36.0	27.0	36.0
46	33.69184521206821	36.0	36.0	36.0	27.0	36.0
47	33.630108166615294	36.0	36.0	36.0	27.0	36.0
48	33.54409456156322	36.0	36.0	36.0	21.0	36.0
49	33.49361680775875	36.0	36.0	36.0	21.0	36.0
50	32.874818109485545	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	19.0
22	50.0
23	187.0
24	523.0
25	1217.0
26	2514.0
27	4825.0
28	7940.0
29	12353.0
30	17318.0
31	24558.0
32	35364.0
33	55778.0
34	109060.0
35	286314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73031120350541	19.06146112420362	12.75549064059714	25.452737031693832
2	13.873367907588536	21.35921982129865	40.53072795891231	24.236684312200506
3	18.756899344831048	26.289191862715583	29.15537682967041	25.79853196278296
4	11.63651286315382	16.74432107349452	37.19204054276846	34.427125520583196
5	12.537901323947903	38.829234375336	34.05581798762786	14.577046313088232
6	32.16749757179773	36.58825932841839	17.73268629060295	13.511556809180933
7	27.90612657429376	31.57529729690983	21.789587619259247	18.728988509537157
8	26.302264402503102	36.241550603195535	19.930720322277732	17.525464672023627
9	27.216945629188917	14.360596394394465	19.484606286513028	38.93785168990359
10	15.564591423650997	28.341663336457458	33.117810556195714	22.975934683695833
11	35.41901123241128	21.186929688116006	24.68926290437039	18.704796175102327
12	25.342097529156245	23.812962790397616	31.18356068154286	19.66137899890328
13	30.503526730033116	21.074720800539044	25.92899230140639	22.492760168021448
14	22.098174285161317	21.53744973370512	26.89434938748593	29.47002659364763
15	23.004908540573865	31.149071606628592	23.740741603599954	22.10527824919759
16	22.131843791664874	26.669103837827763	28.64733416483879	22.55171820566857
17	21.491215494562997	27.848602579082055	28.09930684481782	22.560875081537127
18	22.055742822479914	25.522958566520977	31.553849544914492	20.867449066084617
19	24.536582393715012	25.221494261936677	27.631050771645864	22.610872572702444
20	24.778010942201306	25.81416895002536	28.395424561353206	21.01239554642013
21	24.40056771344807	25.094439705534487	26.628950518611973	23.87604206240547
22	24.198649886116623	27.109082535432872	27.069657919778074	21.622609658672427
23	21.855293221629854	27.185878768872364	28.361632543344832	22.59719546615295
24	23.858787688723623	26.100085121634336	28.52184041933605	21.51928677030599
25	23.143477193428897	26.59837747460212	28.37394425637837	21.884201075590617
26	21.14676391788604	27.680755548785406	29.36927088466949	21.80320964865906
27	22.290283731666406	27.294477340176922	28.35793344707451	22.057305481082164
28	21.42503844044688	27.626226241294898	27.890918735640835	23.05781658261739
29	22.288230360637407	26.18366630108313	27.823236776270043	23.704866562009418
30	21.67547186041677	26.917907398513275	28.549616131784	22.857004609285955
31	23.184109739290328	26.024907836702948	27.786808416834386	23.004174007172338
32	21.794809761132885	27.82374477831003	27.827687367049634	22.553758093507454
33	21.28144976729099	27.628662008304765	29.069181871457168	22.02070635294708
34	22.26628722576853	27.570813635500187	28.86432956879218	21.298569569939104
35	22.205775092873516	27.862950411905015	28.428345372990428	21.502929122231045
36	21.381475779728056	27.764286725581243	28.794604853552887	22.059632641137814
37	22.110494227619256	27.650976161375766	27.068182551317022	23.170347059687955
38	22.314738315462353	28.592371424833463	26.941598369836967	22.151291889867217
39	23.342927086282845	26.48055024892384	27.642553252497304	22.533969412296013
40	24.190204135760766	26.85610140301894	27.76898663288	21.1847078283403
41	22.929943154529358	26.436011211389165	28.967892703278114	21.666152930803364
42	23.081982254830102	28.663592653191422	27.586194537383673	20.668230554594803
43	21.378982832156552	28.197914053259737	28.312067667825524	22.11103544675818
44	21.178555684184495	28.387948862231237	27.53654873822179	22.896946715362475
45	21.502173765003995	28.72452143120831	27.176005075105103	22.59729972868259
46	21.76781877384543	26.776770049872674	27.669388786443523	23.786022389838376
47	23.562048400045875	26.84224108269297	27.178432159651333	22.417278357609817
48	21.93047536100986	28.09855596056041	26.444666659498438	23.52630201893129
49	22.76224183504323	27.43712199274226	26.96259128175261	22.838044890461898
50	21.74233273956941	29.500091394246102	25.803642150309486	22.953933715875003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	44.5
2	37.0
3	137.0
4	237.0
5	290.0
6	343.0
7	334.0
8	325.0
9	401.5
10	478.0
11	711.0
12	944.0
13	1624.5
14	2305.0
15	3070.0
16	3835.0
17	4074.0
18	4313.0
19	4349.5
20	4386.0
21	4662.0
22	4938.0
23	5181.0
24	5424.0
25	5563.5
26	5703.0
27	7375.5
28	9048.0
29	11072.0
30	13096.0
31	15862.0
32	18628.0
33	23036.5
34	27445.0
35	31489.5
36	35534.0
37	35596.5
38	35659.0
39	33697.0
40	31735.0
41	30624.5
42	29514.0
43	28992.0
44	28470.0
45	30326.0
46	32182.0
47	34049.5
48	35917.0
49	35588.0
50	35259.0
51	33396.5
52	31534.0
53	30642.5
54	29751.0
55	29344.5
56	28938.0
57	27496.5
58	26055.0
59	23835.0
60	21615.0
61	19194.5
62	16774.0
63	14448.5
64	12123.0
65	10112.5
66	8102.0
67	6948.5
68	5795.0
69	5077.0
70	4359.0
71	3445.0
72	2531.0
73	2118.5
74	1706.0
75	1383.0
76	1060.0
77	905.5
78	751.0
79	611.0
80	471.0
81	378.0
82	285.0
83	231.5
84	178.0
85	137.0
86	96.0
87	66.5
88	37.0
89	27.5
90	18.0
91	18.5
92	19.0
93	13.5
94	8.0
95	8.0
96	8.0
97	6.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005913681750736522
2	3.5840495459009224E-4
3	7.168099091801845E-4
4	0.0
5	0.0
6	3.5840495459009224E-4
7	0.0
8	0.0
9	0.001433619818360369
10	8.960123864752306E-4
11	0.0
12	0.0
13	7.168099091801845E-4
14	0.0
15	0.0037632520231959685
16	7.168099091801845E-4
17	0.0
18	1.7920247729504612E-4
19	0.0
20	8.960123864752306E-4
21	0.0
22	1.7920247729504612E-4
23	5.376074318851384E-4
24	5.376074318851384E-4
25	0.0016128222956554152
26	0.004121656977786061
27	0.006451289182621661
28	0.003942454500491015
29	0.002867239636720738
30	0.004300859455081107
31	0.009139326342047352
32	0.003404847068605876
33	0.0062720867053266145
34	0.0025088346821306455
35	0.0019712272502455074
36	0.008064111478277076
37	0.0046592644096711995
38	0.008422516432867169
39	0.0046592644096711995
40	0.003046442114015784
41	0.004300859455081107
42	0.003046442114015784
43	0.001433619818360369
44	0.0010752148637702768
45	0.0017920247729504611
46	0.0012544173410653228
47	0.0021504297275405536
48	0.0017920247729504611
49	5.376074318851384E-4
50	0.0010752148637702768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	558028.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.86038794754695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81491426029717	57.715275117544486
2	11.007228605769887	15.159240640413016
3	2.5897293592801156	5.34989305077543
4	0.9174894604780363	2.527147207452125
5	0.45971354102700684	1.5828026389930119
6	0.2592523908135027	1.0711332124648114
7	0.1527491118863501	0.7362854172196126
8	0.1086868892999408	0.5987377089604812
9	0.08718268097896224	0.5403089911066707
>10	0.5105063810540725	6.847634672825394
>50	0.05568526382582591	2.665613406087183
>100	0.03450915814306126	4.109042988547632
>500	0.002352897146115033	1.0968849476102045
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	894	0.16020701470177123	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	867	0.155368547814805	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	785	0.1406739446766112	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	635	0.11379357308235429	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	597	0.10698387894514252	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	597	0.10698387894514252	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	580	0.10393743683112676	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	579	0.10375823435383172	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	559	0.10017418480793079	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.376074318851384E-4
13	0.0	0.0	0.0	0.0	5.376074318851384E-4
14	0.0	0.0	0.0	0.0	5.376074318851384E-4
15	0.0	0.0	0.0	0.0	7.168099091801845E-4
16	0.0	0.0	0.0	0.0	7.168099091801845E-4
17	1.7920247729504612E-4	0.0	0.0	0.0	7.168099091801845E-4
18	1.7920247729504612E-4	0.0	0.0	1.7920247729504612E-4	7.168099091801845E-4
19	1.7920247729504612E-4	0.0	0.0	3.5840495459009224E-4	7.168099091801845E-4
20	1.7920247729504612E-4	0.0	0.0	5.376074318851384E-4	7.168099091801845E-4
21	1.7920247729504612E-4	0.0	0.0	7.168099091801845E-4	7.168099091801845E-4
22	1.7920247729504612E-4	0.0	0.0	0.0010752148637702768	7.168099091801845E-4
23	1.7920247729504612E-4	0.0	0.0	0.0021504297275405536	7.168099091801845E-4
24	1.7920247729504612E-4	0.0	0.0	0.004480061932376153	7.168099091801845E-4
25	1.7920247729504612E-4	0.0	0.0	0.006809694137211753	7.168099091801845E-4
26	1.7920247729504612E-4	0.0	0.0	0.009497731296637444	7.168099091801845E-4
27	1.7920247729504612E-4	0.0	0.0	0.012185768456063137	7.168099091801845E-4
28	1.7920247729504612E-4	0.0	0.0	0.019174665070569936	7.168099091801845E-4
29	1.7920247729504612E-4	0.0	0.0	0.034586078117943904	7.168099091801845E-4
30	1.7920247729504612E-4	0.0	0.0	0.06469209430351165	7.168099091801845E-4
31	1.7920247729504612E-4	0.0	0.0	0.11486878794612457	8.960123864752307E-4
32	1.7920247729504612E-4	0.0	0.0	0.1729303905897195	8.960123864752307E-4
33	1.7920247729504612E-4	0.0	0.0	0.22436150157339774	8.960123864752307E-4
34	1.7920247729504612E-4	0.0	0.0	0.27830144723920663	8.960123864752307E-4
35	1.7920247729504612E-4	0.0	0.0	0.3446063638383737	8.960123864752307E-4
36	1.7920247729504612E-4	0.0	0.0	0.4284731232124553	8.960123864752307E-4
37	1.7920247729504612E-4	0.0	0.0	0.5540940597962827	8.960123864752307E-4
38	1.7920247729504612E-4	0.0	0.0	0.7211107686352656	8.960123864752307E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	1085	0.0	22.504345	2
TAATACC	50	0.002579079	21.999462	4
CATGCGG	50	0.002579079	21.999462	10
GCGGCGT	70	3.211959E-5	21.999462	40
ACGACGG	70	3.211959E-5	21.999462	6
GTCCTAG	70	3.214182E-5	21.99749	1
GCGTTAT	70	3.214182E-5	21.99749	43
TATTATG	70	3.214182E-5	21.99749	2
TAGGACC	470	0.0	21.531387	4
GATATAC	420	0.0	21.473742	1
CTGTAGG	1105	0.0	21.101665	1
ATACACT	430	0.0	20.97623	4
GCTAAGA	95	6.860191E-7	20.839727	1
GTAGGAC	1105	0.0	20.705376	3
TCGTTTA	75	5.4593584E-5	20.53651	30
TACGACG	75	5.4668988E-5	20.532831	5
CGAATGT	280	0.0	20.433565	33
AGTGTAT	295	0.0	20.135101	10
AACGAAT	275	0.0	20.003096	31
GTATAGG	55	0.0044835974	19.997719	1
>>END_MODULE