FastQCFastQC Report
Thu 2 Feb 2017
SRR4064220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946598
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25700.27149856644531256No Hit
TATCAACGCAGAGTACTTTTTTTTT20160.21297319453453312No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15100.159518612969814No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11690.1234948732196772No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11170.1180015170114452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA601.4603611E-615.838490516
AACCGCG954.8912625E-914.0001177
CGACGGG759.620016E-713.93860914
GTCCTAC852.7088754E-713.4062041
CGTCGTA1351.8189894E-1213.37118810
AAGACGG1500.013.295195
ACGGTAT1950.013.1546519
TATTAAG655.488342E-513.143532
GGGCGCT957.3483534E-813.00354718
CCGATAA751.4762036E-512.6674429
CGATAAC751.4762036E-512.66744210
ACCGTCG1352.7284841E-1112.6674418
GAACCGC1207.421477E-1012.6634246
GTATTAG1800.012.6614161
AATTCCG853.9546667E-612.2896715
AGACGGA1950.012.176376
AGACCGA550.00307280412.0878146
AGAACCG1103.8255166E-812.0865365
TAATCTG550.003075238612.0865365
CAAGACG1650.012.0852584
Produced by FastQC (version 0.11.5)