##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064220_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	946598
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118843479491822	32.0	32.0	32.0	32.0	32.0
2	30.83969224528258	32.0	32.0	32.0	32.0	32.0
3	30.849803189949693	32.0	32.0	32.0	32.0	32.0
4	30.87935110786205	32.0	32.0	32.0	32.0	32.0
5	30.808475192214647	32.0	32.0	32.0	32.0	32.0
6	34.29466679625353	36.0	36.0	36.0	32.0	36.0
7	34.28396742862334	36.0	36.0	36.0	32.0	36.0
8	34.23739221929478	36.0	36.0	36.0	32.0	36.0
9	34.36487822708267	36.0	36.0	36.0	32.0	36.0
10	34.14338821759606	36.0	36.0	36.0	32.0	36.0
11	34.3248115884462	36.0	36.0	36.0	32.0	36.0
12	34.188435851332876	36.0	36.0	36.0	32.0	36.0
13	34.27496149368581	36.0	36.0	36.0	32.0	36.0
14	34.19088250767485	36.0	36.0	36.0	32.0	36.0
15	34.12458403672942	36.0	36.0	36.0	32.0	36.0
16	34.114907278485695	36.0	36.0	36.0	32.0	36.0
17	34.06083997642082	36.0	36.0	36.0	32.0	36.0
18	34.05474763310296	36.0	36.0	36.0	32.0	36.0
19	34.05181080036087	36.0	36.0	36.0	32.0	36.0
20	34.018363655955326	36.0	36.0	36.0	32.0	36.0
21	33.9989097800756	36.0	36.0	36.0	32.0	36.0
22	33.97564330370442	36.0	36.0	36.0	32.0	36.0
23	33.94765254099417	36.0	36.0	36.0	32.0	36.0
24	33.92727430229094	36.0	36.0	36.0	32.0	36.0
25	33.41205031069155	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	10.0
6	33.0
7	11.0
8	49.0
9	44.0
10	86.0
11	24.0
12	63.0
13	72.0
14	88.0
15	219.0
16	336.0
17	591.0
18	728.0
19	870.0
20	1209.0
21	1596.0
22	2210.0
23	3264.0
24	4519.0
25	6438.0
26	9167.0
27	12710.0
28	17895.0
29	25239.0
30	35037.0
31	50663.0
32	76351.0
33	111032.0
34	232698.0
35	353341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10011138752502	17.610114071816422	11.390484081306553	25.89929045935201
2	16.41748313362731	20.12111249389895	37.60952841769865	25.85187595477509
3	18.62758044392985	23.940434675588506	28.727401802890583	28.70458307759106
4	12.32864556041987	15.2168447118879	36.39295735366204	36.061552374030185
5	14.168534019631771	36.72943296915989	34.132782266116024	14.969250745092321
6	33.705444637085826	36.0099567771907	16.895353468759872	13.389245116963606
7	29.852600881224838	30.66282055345992	21.14485265371245	18.339725911602795
8	27.83244672419587	33.159076066625666	19.686104436004573	19.32237277317389
9	27.132716949704278	14.034564832312368	18.995966872550635	39.83675134543272
10	15.158058733500756	27.17935871743487	32.55096691272841	25.111615636335966
11	36.795509100075996	21.354605423791156	22.661456456091432	19.18842902004141
12	23.9012664623724	23.976211944555143	29.654415705359604	22.468105887712852
13	28.90661254433116	19.78192504267948	25.695530150474895	25.61593226251447
14	22.82860102750587	19.673037484936255	26.206790841244	31.291570646313875
15	24.765840948369263	27.37385642332024	22.856100925431665	25.004201702878824
16	24.97293800265546	26.221595473880942	24.31319610645513	24.492270417008466
17	23.407838059247908	26.173515499061867	25.883776855791336	24.534869585898893
18	24.061796618376384	25.493272381081262	26.69871452630733	23.74621647423502
19	24.871179524046134	25.330958145238874	26.20209538106968	23.59576694964531
20	25.035338546980444	24.81056741495225	25.719378633632573	24.434715404434737
21	26.020741492499443	24.622223631769792	25.279777573393382	24.077257302337383
22	24.930354901904813	24.74523373793567	25.763030108884227	24.561381251275286
23	23.94719564065885	24.56813109318684	26.139342017760043	25.34533124839427
24	24.018082173243798	25.391265921277423	26.01691080701636	24.573741098462424
25	24.314606503996277	24.788239522983886	26.045270013109484	24.85188395991035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	65.0
2	231.0
3	397.0
4	397.0
5	397.0
6	1272.0
7	2147.0
8	2147.0
9	2147.0
10	2357.5
11	2568.0
12	2568.0
13	2568.0
14	2839.0
15	3110.0
16	3110.0
17	3110.0
18	5277.0
19	7444.0
20	7444.0
21	7444.0
22	11807.0
23	16170.0
24	16170.0
25	16170.0
26	23084.5
27	29999.0
28	29999.0
29	29999.0
30	36953.0
31	43907.0
32	43907.0
33	43907.0
34	53598.0
35	63289.0
36	63289.0
37	63289.0
38	72276.5
39	81264.0
40	81264.0
41	81264.0
42	91834.0
43	102404.0
44	102404.0
45	102404.0
46	113051.0
47	123698.0
48	123698.0
49	123698.0
50	125632.5
51	127567.0
52	127567.0
53	127567.0
54	117867.5
55	108168.0
56	108168.0
57	108168.0
58	97957.5
59	87747.0
60	87747.0
61	87747.0
62	75740.5
63	63734.0
64	63734.0
65	63734.0
66	52124.5
67	40515.0
68	40515.0
69	40515.0
70	30514.0
71	20513.0
72	20513.0
73	20513.0
74	15641.5
75	10770.0
76	10770.0
77	10770.0
78	8590.0
79	6410.0
80	6410.0
81	6410.0
82	4358.5
83	2307.0
84	2307.0
85	2307.0
86	1799.5
87	1292.0
88	1292.0
89	1292.0
90	892.5
91	493.0
92	493.0
93	493.0
94	329.5
95	166.0
96	166.0
97	166.0
98	310.0
99	454.0
100	454.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037185795871108956
2	0.004225658621716927
3	7.394902588004623E-4
4	0.0023241122419443098
5	0.007500544053547547
6	0.011303636813092781
7	0.01986059552206956
8	0.03126987380070526
9	0.04584839604562867
10	0.052186883978204056
11	0.053560223030262054
12	0.06095512561826667
13	0.06264538906695344
14	0.06528642570552652
15	0.05841973044523652
16	0.06570899156769822
17	0.06053255975609498
18	0.07616749665644762
19	0.07479415760438962
20	0.07891417476056362
21	0.07056849898267269
22	0.07722391131187685
23	0.08102700407142208
24	0.0733151770867887
25	0.07585057225981885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	946598.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.07742803381997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55575526242697	48.39582589488471
2	11.952718661262567	14.361771903609979
3	3.307562144489911	5.961294801089396
4	1.3588147120859555	3.2653637230655916
5	0.7235319232566038	2.173396852480998
6	0.4435809509669848	1.5989521595335472
7	0.3100401015900124	1.303848832361054
8	0.2137943500140141	1.02753717416034
9	0.1593265558199196	0.8614736722032859
>10	0.8456167254512623	9.103701208420333
>50	0.06525094543820219	2.77599345422665
>100	0.05959942581042688	6.869840918385366
>500	0.003526593109859375	1.4130579195716058
>1k	8.816482774648437E-4	0.8879414860071371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2570	0.27149856644531256	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2016	0.21297319453453312	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1510	0.159518612969814	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1169	0.1234948732196772	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1117	0.1180015170114452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.0564146554292318E-4
6	0.0	0.0	0.0	0.0	1.0564146554292318E-4
7	0.0	0.0	0.0	0.0	1.0564146554292318E-4
8	0.0	0.0	0.0	0.0	1.0564146554292318E-4
9	0.0	0.0	0.0	0.0	1.0564146554292318E-4
10	0.0	0.0	0.0	0.0	1.0564146554292318E-4
11	0.0	0.0	0.0	0.0	1.0564146554292318E-4
12	0.0	0.0	0.0	0.0	2.1128293108584636E-4
13	0.0	0.0	0.0	0.0	2.1128293108584636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	60	1.4603611E-6	15.8384905	16
AACCGCG	95	4.8912625E-9	14.000117	7
CGACGGG	75	9.620016E-7	13.938609	14
GTCCTAC	85	2.7088754E-7	13.406204	1
CGTCGTA	135	1.8189894E-12	13.371188	10
AAGACGG	150	0.0	13.29519	5
ACGGTAT	195	0.0	13.154651	9
TATTAAG	65	5.488342E-5	13.14353	2
GGGCGCT	95	7.3483534E-8	13.003547	18
CCGATAA	75	1.4762036E-5	12.667442	9
CGATAAC	75	1.4762036E-5	12.667442	10
ACCGTCG	135	2.7284841E-11	12.667441	8
GAACCGC	120	7.421477E-10	12.663424	6
GTATTAG	180	0.0	12.661416	1
AATTCCG	85	3.9546667E-6	12.289671	5
AGACGGA	195	0.0	12.17637	6
AGACCGA	55	0.003072804	12.087814	6
AGAACCG	110	3.8255166E-8	12.086536	5
TAATCTG	55	0.0030752386	12.086536	5
CAAGACG	165	0.0	12.085258	4
>>END_MODULE