FastQCFastQC Report
Thu 2 Feb 2017
SRR4064220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946598
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA551.5932879E-423.9999439
CGTACTA500.002581647121.9976233
GTATCAA18500.019.14591
GGCGTAC708.1216736E-418.85711
CCGTCGT2100.018.8570989
TGAACCG600.00741556318.3313545
GACCCGT600.00741556318.3313546
ATAGTAC1102.7980095E-617.9980553
CATCGTT2700.017.92588228
CGTCGTA2350.017.78719110
CGACGAA750.001289696417.60181632
ACCGTCG2250.017.5980998
TAAGACT2150.017.3934694
CGCGGGA2300.017.21734844
ATTATAC1154.275307E-617.215533
ATACCGT2300.017.215536
TACCGTC2450.017.059387
ATCGTTT2900.016.69049629
TAACGCC2900.016.687854
ATAACGC3050.016.5883713
Produced by FastQC (version 0.11.5)