##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	946598
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.010329622500787	32.0	32.0	32.0	32.0	32.0
2	30.79109822754749	32.0	32.0	32.0	32.0	32.0
3	31.12409491674396	32.0	32.0	32.0	32.0	32.0
4	31.322693477062067	32.0	32.0	32.0	32.0	32.0
5	31.172333979154825	32.0	32.0	32.0	32.0	32.0
6	34.726585097369735	36.0	36.0	36.0	32.0	36.0
7	34.77675845501469	36.0	36.0	36.0	32.0	36.0
8	34.66929995626443	36.0	36.0	36.0	32.0	36.0
9	34.89286370771964	36.0	36.0	36.0	32.0	36.0
10	34.61081261528125	36.0	36.0	36.0	32.0	36.0
11	34.89001244456464	36.0	36.0	36.0	32.0	36.0
12	34.7359554953634	36.0	36.0	36.0	32.0	36.0
13	34.821529308111785	36.0	36.0	36.0	32.0	36.0
14	34.74953570575894	36.0	36.0	36.0	32.0	36.0
15	34.67937287000395	36.0	36.0	36.0	32.0	36.0
16	34.72304822110336	36.0	36.0	36.0	32.0	36.0
17	34.66814740787536	36.0	36.0	36.0	32.0	36.0
18	34.67430102324324	36.0	36.0	36.0	32.0	36.0
19	34.66525917020741	36.0	36.0	36.0	32.0	36.0
20	34.671904018390066	36.0	36.0	36.0	32.0	36.0
21	34.657127946604575	36.0	36.0	36.0	32.0	36.0
22	34.63496119788971	36.0	36.0	36.0	32.0	36.0
23	34.57086957715947	36.0	36.0	36.0	32.0	36.0
24	34.55339542234402	36.0	36.0	36.0	32.0	36.0
25	34.52225337471662	36.0	36.0	36.0	32.0	36.0
26	34.44505481735647	36.0	36.0	36.0	32.0	36.0
27	34.440777394416635	36.0	36.0	36.0	32.0	36.0
28	34.408342295250996	36.0	36.0	36.0	32.0	36.0
29	34.38355563819066	36.0	36.0	36.0	32.0	36.0
30	34.351309637248335	36.0	36.0	36.0	32.0	36.0
31	34.34577930652716	36.0	36.0	36.0	32.0	36.0
32	34.29544326102527	36.0	36.0	36.0	32.0	36.0
33	34.27357653407254	36.0	36.0	36.0	32.0	36.0
34	34.250392458044495	36.0	36.0	36.0	32.0	36.0
35	34.22470151003911	36.0	36.0	36.0	32.0	36.0
36	34.181359985970815	36.0	36.0	36.0	32.0	36.0
37	34.16342100870697	36.0	36.0	36.0	32.0	36.0
38	34.12625634112897	36.0	36.0	36.0	32.0	36.0
39	34.12848326322261	36.0	36.0	36.0	32.0	36.0
40	34.10207817891016	36.0	36.0	36.0	32.0	36.0
41	34.07456068996554	36.0	36.0	36.0	32.0	36.0
42	34.00029790893283	36.0	36.0	36.0	32.0	36.0
43	33.99595815752833	36.0	36.0	36.0	32.0	36.0
44	33.93087984550992	36.0	36.0	36.0	32.0	36.0
45	33.88193404169457	36.0	36.0	36.0	32.0	36.0
46	33.86786259848426	36.0	36.0	36.0	32.0	36.0
47	33.84527222749256	36.0	36.0	36.0	32.0	36.0
48	33.79487702276996	36.0	36.0	36.0	32.0	36.0
49	33.76490125692216	36.0	36.0	36.0	32.0	36.0
50	33.034600749209275	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	6.0
21	24.0
22	84.0
23	298.0
24	835.0
25	1908.0
26	3977.0
27	7617.0
28	12966.0
29	21104.0
30	31738.0
31	47292.0
32	71485.0
33	116933.0
34	219514.0
35	410812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.543020022481976	18.840276545213282	12.163509892914796	26.453193539389947
2	15.805158694944835	21.036746405013332	36.68703438425685	26.471060515784984
3	18.0679845805554	24.60931103732634	29.150419872448357	28.172284509669904
4	12.386250551976657	16.2272685976518	36.46109541748451	34.92538543288703
5	14.279451255971384	36.90732496793782	33.38555543113338	15.427668344957416
6	34.12698328855891	36.569733476865025	16.24862533897741	13.054657895598654
7	29.897939672320955	30.864031895304972	20.893157278123635	18.344871154250438
8	27.876564286001027	33.589760384027855	19.258016602612724	19.275658727358394
9	27.166460664067866	13.855946080140294	18.44388806135708	40.53370519443476
10	14.946851554098167	27.505456404752195	32.38157013460892	25.166121906540713
11	37.38778235322703	21.158929133592082	22.344437659914767	19.10885085326612
12	23.862868057198877	24.08608988877996	29.339145059328626	22.71189699469254
13	29.21774442317525	19.72393713896953	25.511833981973304	25.546484455881917
14	22.98010663460797	19.524148079911516	25.895391597480238	31.600353688000276
15	24.876050258147192	27.507492977149724	22.484213773555204	25.13224299114788
16	25.286132327618084	26.361677178081322	23.83936022987777	24.51283026442282
17	23.65502568144027	26.28475868320026	25.587419369151426	24.472796266208043
18	24.392717469157844	25.529952651295062	26.335683513734505	23.74164636581259
19	25.176474068189453	25.39377856281125	25.818457254293797	23.6112901147055
20	25.30424806358785	24.832557923337937	25.34724423090738	24.51594978216684
21	26.181863895761452	24.65376009668307	25.056148438936066	24.108227568619416
22	25.15751175791996	24.83699487426526	25.444751509619735	24.560741858195048
23	24.216794793104942	24.533404606635813	25.923656573958553	25.326144026300696
24	24.24381679195771	25.34861757286107	25.701566454960723	24.705999180220495
25	24.612922309788924	24.831181727904667	25.72672146041539	24.829174501891018
26	24.334477840578945	25.550367122708785	25.974222175268082	24.140932861444192
27	24.800617823271693	25.218451448366647	25.330542845642434	24.650387882719222
28	24.145388085403084	25.130206957752726	26.201548749696276	24.522856207147914
29	24.262265986745984	25.200379048385717	25.860861352594235	24.67649361227407
30	24.236924286154977	25.564673915340112	25.958308154546337	24.24009364395857
31	24.599512960429358	25.339686329612743	25.244180088427793	24.81662062153011
32	24.24009364395857	25.485123034469936	25.229144569199757	25.045638752371737
33	24.17593794800335	25.074876315419715	25.72406849637794	25.025117240198995
34	24.603331392996406	25.07091385643549	25.920084177520756	24.40567057304735
35	25.217257182217136	25.097351078732355	25.5245759972279	24.160815741822613
36	24.3608733410387	25.645993876554897	25.357676387125128	24.63545639528127
37	25.00409374950479	25.383571122955107	25.323036452858496	24.28929867468161
38	24.397165218567228	25.121919267047566	25.815823726292304	24.665091788092905
39	24.83091440391649	25.24599550795405	25.108233797680445	24.814856290449015
40	24.869635401700442	25.485750747958956	25.573857777928026	24.070756072412568
41	24.02912826493917	25.762435398469425	25.871988607311437	24.33644772927997
42	25.09309801676134	25.67424092763128	25.288009609299976	23.944651446307404
43	24.45778830449637	25.151333317839054	25.572214252059506	24.818664125605068
44	24.33017179632912	25.512420424557302	25.25856076468488	24.898847014428693
45	24.398257312610397	25.743515630942422	25.22597043685505	24.632256619592134
46	24.345838179661165	25.488462306397786	25.309082150406777	24.856617363534276
47	24.41161991248513	25.386707120988987	25.743570023516188	24.458102943009692
48	25.106092571248677	25.808622418202816	24.83532806030981	24.2499569502387
49	24.41400555252716	25.87376240761634	25.076484582651943	24.635747457204552
50	24.542910477325382	25.7841064793833	24.98872264250407	24.68426040078725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	20.0
2	19.0
3	51.5
4	84.0
5	110.0
6	136.0
7	143.0
8	150.0
9	214.5
10	279.0
11	398.0
12	517.0
13	960.5
14	1404.0
15	1886.5
16	2369.0
17	2598.0
18	2827.0
19	3016.5
20	3206.0
21	3850.0
22	4494.0
23	5173.0
24	5852.0
25	6796.0
26	7740.0
27	9262.0
28	10784.0
29	12391.0
30	13998.0
31	15792.0
32	17586.0
33	19628.0
34	21670.0
35	24820.0
36	27970.0
37	32366.5
38	36763.0
39	40189.0
40	43615.0
41	47744.5
42	51874.0
43	53090.5
44	54307.0
45	58988.5
46	63670.0
47	66832.5
48	69995.0
49	71436.5
50	72878.0
51	70957.5
52	69037.0
53	67250.5
54	65464.0
55	65022.5
56	64581.0
57	62308.5
58	60036.0
59	54196.5
60	48357.0
61	42417.0
62	36477.0
63	31854.0
64	27231.0
65	23156.5
66	19082.0
67	16335.5
68	13589.0
69	12187.0
70	10785.0
71	8451.5
72	6118.0
73	5239.5
74	4361.0
75	3479.0
76	2597.0
77	2211.0
78	1825.0
79	1520.5
80	1216.0
81	983.0
82	750.0
83	586.5
84	423.0
85	318.0
86	213.0
87	151.5
88	90.0
89	71.0
90	52.0
91	38.0
92	24.0
93	25.5
94	27.0
95	25.0
96	23.0
97	19.0
98	15.0
99	16.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006549770863661238
2	2.112829310858464E-4
3	1.056414655429232E-4
4	0.0
5	0.0
6	1.056414655429232E-4
7	1.056414655429232E-4
8	0.0
9	8.451317243433856E-4
10	4.225658621716928E-4
11	0.0
12	6.338487932575391E-4
13	2.112829310858464E-4
14	1.056414655429232E-4
15	0.004753865949431543
16	8.451317243433856E-4
17	0.0
18	4.225658621716928E-4
19	0.0
20	2.112829310858464E-4
21	0.0
22	2.112829310858464E-4
23	7.394902588004623E-4
24	2.112829310858464E-4
25	0.0019015463797726173
26	0.005070790346060313
27	0.005176431811603236
28	0.002957961035201849
29	0.001795904914229694
30	0.003591809828459388
31	0.00665541232920416
32	0.003591809828459388
33	0.003908734225088158
34	0.002852319569658926
35	0.0025353951730301564
36	0.007606185519090469
37	0.0034861683629164655
38	0.0073949025880046225
39	0.0038030927595452345
40	0.0023241122419443098
41	0.002746678104116003
42	0.0015846219831438476
43	0.0012676975865150782
44	0.0012676975865150782
45	0.0014789805176009245
46	5.282073277146159E-4
47	0.0016902634486867711
48	0.0020071878453155408
49	2.112829310858464E-4
50	0.0011620561209721549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	946598.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.02497936836653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9364801970672	52.459814541338
2	11.338586800923535	14.519055719911247
3	3.055448701777819	5.868751202772815
4	1.2355685456095555	3.1642900256341777
5	0.6405333814693329	2.0505068266662043
6	0.38428983648477	1.476248931144621
7	0.24881655196835314	1.1151332224397337
8	0.19821114499650622	1.0152371575185293
9	0.1440125568271396	0.8298360881678991
>10	0.7121125452863013	8.149244200755748
>50	0.05845471446857714	2.647861491313595
>100	0.044496455429342566	5.464436955819727
>500	0.002988567691639711	1.2395836365176678
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1128293108584636E-4
2	0.0	0.0	0.0	0.0	2.1128293108584636E-4
3	0.0	0.0	0.0	0.0	2.1128293108584636E-4
4	0.0	0.0	0.0	0.0	2.1128293108584636E-4
5	0.0	0.0	0.0	0.0	2.1128293108584636E-4
6	0.0	0.0	0.0	0.0	2.1128293108584636E-4
7	0.0	0.0	0.0	0.0	2.1128293108584636E-4
8	0.0	0.0	0.0	0.0	2.1128293108584636E-4
9	0.0	0.0	0.0	0.0	3.1692439662876954E-4
10	0.0	0.0	0.0	0.0	3.1692439662876954E-4
11	0.0	0.0	0.0	0.0	3.1692439662876954E-4
12	0.0	0.0	0.0	0.0	4.225658621716927E-4
13	0.0	0.0	0.0	0.0	5.282073277146159E-4
14	0.0	0.0	0.0	0.0	5.282073277146159E-4
15	0.0	0.0	0.0	0.0	6.338487932575391E-4
16	0.0	0.0	0.0	0.0	6.338487932575391E-4
17	0.0	0.0	0.0	0.0	6.338487932575391E-4
18	0.0	0.0	0.0	0.0	6.338487932575391E-4
19	0.0	0.0	0.0	0.0	6.338487932575391E-4
20	0.0	0.0	0.0	0.0	8.451317243433855E-4
21	0.0	0.0	0.0	0.0	8.451317243433855E-4
22	0.0	0.0	0.0	3.1692439662876954E-4	8.451317243433855E-4
23	0.0	0.0	0.0	0.0010564146554292318	8.451317243433855E-4
24	0.0	0.0	0.0	0.0012676975865150782	8.451317243433855E-4
25	0.0	0.0	0.0	0.0012676975865150782	8.451317243433855E-4
26	0.0	0.0	0.0	0.0015846219831438478	9.507731898863086E-4
27	0.0	0.0	0.0	0.002746678104116003	9.507731898863086E-4
28	0.0	0.0	0.0	0.00496514888051739	9.507731898863086E-4
29	0.0	0.0	0.0	0.007077978191375853	9.507731898863086E-4
30	0.0	0.0	0.0	0.013416466123951244	9.507731898863086E-4
31	0.0	0.0	0.0	0.02693857371344541	9.507731898863086E-4
32	0.0	0.0	0.0	0.04215094475162635	9.507731898863086E-4
33	0.0	0.0	0.0	0.05630690113437806	9.507731898863086E-4
34	0.0	0.0	0.0	0.07585057225981885	9.507731898863086E-4
35	0.0	0.0	0.0	0.09602809217851717	9.507731898863086E-4
36	0.0	0.0	0.0	0.1271923245136795	9.507731898863086E-4
37	0.0	0.0	0.0	0.17525919133570955	9.507731898863086E-4
38	0.0	0.0	0.0	0.243397936610895	9.507731898863086E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	55	1.5932879E-4	23.999943	9
CGTACTA	50	0.0025816471	21.997623	3
GTATCAA	1850	0.0	19.1459	1
GGCGTAC	70	8.1216736E-4	18.8571	1
CCGTCGT	210	0.0	18.857098	9
TGAACCG	60	0.007415563	18.331354	5
GACCCGT	60	0.007415563	18.331354	6
ATAGTAC	110	2.7980095E-6	17.998055	3
CATCGTT	270	0.0	17.925882	28
CGTCGTA	235	0.0	17.787191	10
CGACGAA	75	0.0012896964	17.601816	32
ACCGTCG	225	0.0	17.598099	8
TAAGACT	215	0.0	17.393469	4
CGCGGGA	230	0.0	17.217348	44
ATTATAC	115	4.275307E-6	17.21553	3
ATACCGT	230	0.0	17.21553	6
TACCGTC	245	0.0	17.05938	7
ATCGTTT	290	0.0	16.690496	29
TAACGCC	290	0.0	16.68785	4
ATAACGC	305	0.0	16.588371	3
>>END_MODULE