FastQCFastQC Report
Thu 2 Feb 2017
SRR4064219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518431
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40830.26889598539545095No Hit
TATCAACGCAGAGTACTTTTTTTTT26690.17577354519237293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16830.11083809537608229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG2300.014.0400934
CGAACGA1101.8553692E-1013.82122516
GCGTTAT1500.013.297671
AAGACGG2400.013.0593525
CGTTATT1301.9826984E-1012.4184472
TACACTT701.0933255E-412.2113415
GTATCAA20500.011.9540031
CGGTAAT907.474684E-611.6090771
CGAGCCG2500.011.40251315
ATACCGT2250.011.3987556
ACGAACG1354.7093636E-911.2617415
TAGAACA1354.7439244E-911.2565454
CGCAAGA2800.011.1922552
GTAATAC855.3391035E-511.173043
CGTCGTA2300.011.15279310
TACCGTC2300.011.15169057
ATAACGA1451.2150849E-911.1385512
CGGTCGG1303.2619937E-810.96214810
CCGATAA1303.2636308E-810.9617859
ACCGTCC700.001491086710.8573888
Produced by FastQC (version 0.11.5)