FastQCFastQC Report
Thu 2 Feb 2017
SRR4064219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518431
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.022.642981
TAGGACG1158.782081E-921.0419984
ACGCGAG550.00448339320.0005727
CTATCGG550.00448339320.000571
GGTATCA13500.018.7412721
CCGAACG600.00741455618.3326459
CGCAATG600.007415964718.3320432
GTATAGG1408.373536E-817.2862071
ATTACAC2700.017.1099073
CGAATGC3550.016.7317743
TAGCGGC3700.016.6496730
AACGCAG36600.016.6491016
TCTAGCG3600.016.5004728
CGCAATA3650.016.27550736
TATCAAC38750.015.9524262
TACGAAT3600.015.88829441
GTATAGT1259.440822E-615.840451
CGGTCCA3750.015.8399310
CGCCGGT3500.015.7131817
CTAGCGG3800.015.63202329
Produced by FastQC (version 0.11.5)