##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1518431
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298570037097505	32.0	32.0	32.0	32.0	32.0
2	31.392119892178176	32.0	32.0	32.0	32.0	32.0
3	31.463147815080173	32.0	32.0	32.0	32.0	32.0
4	31.545274036159693	32.0	32.0	32.0	32.0	32.0
5	31.508456426403306	32.0	32.0	32.0	32.0	32.0
6	35.071770794985085	36.0	36.0	36.0	36.0	36.0
7	35.084503016600685	36.0	36.0	36.0	36.0	36.0
8	35.03392054034724	36.0	36.0	36.0	36.0	36.0
9	35.135367362758004	36.0	36.0	36.0	36.0	36.0
10	34.99685925801041	36.0	36.0	36.0	36.0	36.0
11	35.13404231078001	36.0	36.0	36.0	36.0	36.0
12	35.04262689578914	36.0	36.0	36.0	36.0	36.0
13	35.08443189055018	36.0	36.0	36.0	36.0	36.0
14	35.025382121413486	36.0	36.0	36.0	36.0	36.0
15	34.99721422968841	36.0	36.0	36.0	36.0	36.0
16	35.01076703518303	36.0	36.0	36.0	36.0	36.0
17	34.97451382380892	36.0	36.0	36.0	36.0	36.0
18	34.968715074968834	36.0	36.0	36.0	36.0	36.0
19	34.97303993398449	36.0	36.0	36.0	36.0	36.0
20	34.96761064546232	36.0	36.0	36.0	36.0	36.0
21	34.961288329861546	36.0	36.0	36.0	36.0	36.0
22	34.944028408271436	36.0	36.0	36.0	36.0	36.0
23	34.899750466106134	36.0	36.0	36.0	32.0	36.0
24	34.86908986974054	36.0	36.0	36.0	32.0	36.0
25	34.84342587842319	36.0	36.0	36.0	32.0	36.0
26	34.77866758515863	36.0	36.0	36.0	32.0	36.0
27	34.772410468437485	36.0	36.0	36.0	32.0	36.0
28	34.758444736705194	36.0	36.0	36.0	32.0	36.0
29	34.73403730561349	36.0	36.0	36.0	32.0	36.0
30	34.712588849937866	36.0	36.0	36.0	32.0	36.0
31	34.69861719103469	36.0	36.0	36.0	32.0	36.0
32	34.6642540885954	36.0	36.0	36.0	32.0	36.0
33	34.63869546920473	36.0	36.0	36.0	32.0	36.0
34	34.63841557502448	36.0	36.0	36.0	32.0	36.0
35	34.597937607965065	36.0	36.0	36.0	32.0	36.0
36	34.56390642709481	36.0	36.0	36.0	32.0	36.0
37	34.552405081297735	36.0	36.0	36.0	32.0	36.0
38	34.52144351636657	36.0	36.0	36.0	32.0	36.0
39	34.527076962996674	36.0	36.0	36.0	32.0	36.0
40	34.49146849609893	36.0	36.0	36.0	32.0	36.0
41	34.47930001429107	36.0	36.0	36.0	32.0	36.0
42	34.4128742102868	36.0	36.0	36.0	32.0	36.0
43	34.39732987537794	36.0	36.0	36.0	32.0	36.0
44	34.34316607076647	36.0	36.0	36.0	32.0	36.0
45	34.30453803959482	36.0	36.0	36.0	32.0	36.0
46	34.288832353923226	36.0	36.0	36.0	32.0	36.0
47	34.25242437753181	36.0	36.0	36.0	32.0	36.0
48	34.206166101719475	36.0	36.0	36.0	32.0	36.0
49	34.188773806646466	36.0	36.0	36.0	32.0	36.0
50	33.59852373930722	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	8.0
20	19.0
21	77.0
22	249.0
23	624.0
24	1543.0
25	3441.0
26	6710.0
27	11723.0
28	18713.0
29	28182.0
30	39416.0
31	54842.0
32	78137.0
33	122465.0
34	252300.0
35	899978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.621488079046664	17.920454088901156	11.74252927465252	26.715528557399658
2	16.12924470272428	20.554628423455405	36.60385584508127	26.71227102873905
3	18.27239067927098	24.32024825773072	28.67002717294665	28.737333890051648
4	12.70212475904404	15.907999770816058	35.52272049240302	35.86715497773689
5	14.395188190968177	36.67924324516557	33.26762954655167	15.657939017314584
6	34.22295953446591	35.404181166311474	16.638984528736298	13.733874770486318
7	30.018657441342334	30.380808058855568	21.053470396047494	18.547064103754604
8	27.959255310251173	32.9667268384273	19.464434011160204	19.609583840161324
9	27.624474946951366	14.020315881397613	18.35074399802953	40.0044651736215
10	15.73411835987408	26.87280199154384	31.930559397268215	25.462520251313865
11	37.359155602065556	21.143667377707647	22.31658863655971	19.18058838366709
12	24.665328882247533	23.42602330958733	28.90404634784195	23.00460146032319
13	29.69083612022513	19.529800094572114	25.192470057652113	25.586893727550645
14	23.69700769480015	19.16811333826826	25.377100527650963	31.757778439280628
15	25.12422951357354	26.92301359559893	22.27819639492373	25.674560495903805
16	25.66027471959358	25.73364040671197	23.582853503738423	25.02323136995603
17	24.24285331371659	25.62480613211927	25.126923778558265	25.00541677560587
18	24.861863535002694	24.98330508039246	25.9290212364646	24.225810148140244
19	25.52101478433989	24.707741082736064	25.584435512710158	24.186808620213892
20	25.850784564501833	24.191232740305395	25.13181447581766	24.826168219375113
21	26.557084253416853	24.12589047510226	24.769251944935267	24.547773326545624
22	25.79402999818891	24.10958723677495	25.163508240446514	24.932874524589625
23	24.671862425234686	24.02480739822631	25.549486373317492	25.75384380322151
24	24.852873381541336	24.697316947880033	25.407397162840322	25.042412507738305
25	25.02171681016116	24.279426689383428	25.428063189825668	25.270793310629745
26	24.809857633619963	24.870120974540548	25.582018736300792	24.738002655538697
27	25.239672796248485	24.759141682912695	25.000197586806472	25.000987934032352
28	24.83913458688708	24.50146540652682	25.652188230645105	25.00721177594099
29	24.76233002521755	24.703254176965427	25.534333473613774	25.000082324203248
30	24.685533037883562	24.959314735672564	25.594735909660603	24.760416316783274
31	25.180019587606463	24.55722064114833	24.965965381177853	25.296794390067355
32	24.888597934369265	24.762410315245123	24.87641384797158	25.472577902414027
33	24.716023412302984	24.443556051416444	25.32576545414191	25.514655082138667
34	25.169309071462486	24.499119783821804	25.477664637965407	24.8539065067503
35	25.55248427753728	24.45657149217351	25.339426713796186	24.651517516493026
36	24.8465720258552	24.93496023847693	25.1209574906705	25.097510244997373
37	25.459313782311348	24.689597669051707	25.006783670716672	24.84430487792027
38	24.82865772775712	24.596359363659236	25.38750423168979	25.187478676893853
39	25.249315378850046	24.61165285130384	24.940363208149858	25.198668561696252
40	25.34436078143142	24.649810688010284	25.317621985875853	24.688206544682437
41	24.63954624846216	24.926368117263053	25.520494426896317	24.91359120737847
42	25.3668335089568	25.02785827186512	25.123880400421495	24.481427818756586
43	24.9858569254306	24.281633892272907	25.340240554105577	25.392268628190916
44	24.69095799399632	24.814508937685805	25.159015860095018	25.335517208222857
45	24.870686419011566	24.907633156436834	25.06299385801652	25.15868656653508
46	24.677542466924436	24.683140402142495	25.25755441357677	25.3817627173563
47	24.792297129534806	24.662291432729955	25.453521646211954	25.091889791523286
48	25.287276080084297	25.186512118018967	24.755202845100104	24.771008956796628
49	24.69572089500634	25.187612163919855	24.879595633633535	25.237071307440274
50	24.74942555078961	25.319692757757945	24.88245936047618	25.048422330976265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	48.5
2	58.0
3	151.5
4	245.0
5	296.5
6	348.0
7	357.0
8	366.0
9	474.5
10	583.0
11	854.0
12	1125.0
13	1995.5
14	2866.0
15	3689.0
16	4512.0
17	4531.0
18	4550.0
19	4489.0
20	4428.0
21	4892.5
22	5357.0
23	6137.0
24	6917.0
25	7853.0
26	8789.0
27	10604.5
28	12420.0
29	14505.5
30	16591.0
31	19185.0
32	21779.0
33	25286.5
34	28794.0
35	33218.0
36	37642.0
37	44327.5
38	51013.0
39	57300.5
40	63588.0
41	70105.5
42	76623.0
43	80874.5
44	85126.0
45	93514.5
46	101903.0
47	108302.0
48	114701.0
49	117992.5
50	121284.0
51	119813.0
52	118342.0
53	116543.5
54	114745.0
55	113278.0
56	111811.0
57	107383.5
58	102956.0
59	94497.5
60	86039.0
61	75323.5
62	64608.0
63	55687.0
64	46766.0
65	39813.0
66	32860.0
67	27980.5
68	23101.0
69	20460.5
70	17820.0
71	13943.5
72	10067.0
73	8509.5
74	6952.0
75	5398.0
76	3844.0
77	3341.0
78	2838.0
79	2319.0
80	1800.0
81	1383.5
82	967.0
83	826.0
84	685.0
85	485.5
86	286.0
87	204.5
88	123.0
89	98.5
90	74.0
91	59.0
92	44.0
93	33.0
94	22.0
95	20.0
96	18.0
97	13.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0090883286761137
2	5.927170875726326E-4
3	5.927170875726326E-4
4	0.0
5	0.0
6	1.9757236252421082E-4
7	1.317149083494739E-4
8	0.0
9	8.561469042715804E-4
10	7.244319959221064E-4
11	0.0
12	0.0
13	8.561469042715804E-4
14	1.9757236252421082E-4
15	0.004741736700581061
16	6.585745417473695E-4
17	0.0
18	3.2928727087368475E-4
19	0.0
20	5.927170875726326E-4
21	0.0
22	3.9514472504842163E-4
23	8.561469042715804E-4
24	7.244319959221064E-4
25	0.0014488639918442128
26	0.006256458146600009
27	0.007310177413395802
28	0.005334453788153693
29	0.0028977279836884255
30	0.004478306883882113
31	0.008166324317667381
32	0.0037538748879600062
33	0.005532026150677904
34	0.003556302525435795
35	0.0040831621588336905
36	0.008495611588541067
37	0.005202738879804219
38	0.00823218177184212
39	0.004807594154755798
40	0.002502583258640004
41	0.004412449429707376
42	0.0020415810794168453
43	0.001317149083494739
44	0.0019098661710673715
45	0.0019098661710673715
46	9.220043584463174E-4
47	0.0023708683502905305
48	0.0020415810794168453
49	3.9514472504842163E-4
50	0.001317149083494739
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1518431.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.78001016304921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19702628598148	48.743760727082794
2	11.882486572196687	14.444353092408207
3	3.444087795668344	6.279950736694673
4	1.4453038376885183	3.513823277736087
5	0.7763658034275001	2.3593760711283664
6	0.4831902613208252	1.76209853962597
7	0.3377669639248581	1.4370635650066605
8	0.23687088253705044	1.151761171834589
9	0.18349147306656702	1.0037352238036934
>10	0.9136319250724713	9.574591616345614
>50	0.05116994183261892	2.1716578417289716
>100	0.04402060318762729	5.5168028367121815
>500	0.004369194376565746	1.8768430285903355
>1k	2.1845971882828737E-4	0.1641822713018712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.585745417473695E-5
2	0.0	0.0	0.0	0.0	6.585745417473695E-5
3	0.0	0.0	0.0	0.0	6.585745417473695E-5
4	0.0	0.0	0.0	0.0	1.317149083494739E-4
5	0.0	0.0	0.0	0.0	1.317149083494739E-4
6	0.0	0.0	0.0	0.0	1.317149083494739E-4
7	0.0	0.0	0.0	0.0	1.317149083494739E-4
8	0.0	0.0	0.0	0.0	1.317149083494739E-4
9	0.0	0.0	0.0	0.0	1.317149083494739E-4
10	0.0	0.0	0.0	0.0	1.317149083494739E-4
11	0.0	0.0	0.0	0.0	1.317149083494739E-4
12	0.0	0.0	0.0	0.0	1.9757236252421084E-4
13	0.0	0.0	0.0	0.0	2.634298166989478E-4
14	0.0	0.0	0.0	0.0	2.634298166989478E-4
15	0.0	0.0	0.0	0.0	3.951447250484217E-4
16	0.0	0.0	0.0	0.0	4.6100217922315863E-4
17	0.0	0.0	0.0	0.0	4.6100217922315863E-4
18	0.0	0.0	0.0	0.0	4.6100217922315863E-4
19	0.0	0.0	0.0	0.0	4.6100217922315863E-4
20	0.0	0.0	0.0	6.585745417473695E-5	4.6100217922315863E-4
21	0.0	0.0	0.0	6.585745417473695E-5	4.6100217922315863E-4
22	0.0	0.0	0.0	3.951447250484217E-4	4.6100217922315863E-4
23	0.0	0.0	0.0	8.561469042715804E-4	5.927170875726325E-4
24	0.0	0.0	0.0	0.001383006537669476	5.927170875726325E-4
25	0.0	0.0	0.0	0.0018440087168926345	5.927170875726325E-4
26	0.0	0.0	0.0	0.00237086835029053	6.585745417473695E-4
27	0.0	0.0	0.0	0.0033587301629115843	6.585745417473695E-4
28	0.0	0.0	0.0	0.006651602871648432	6.585745417473695E-4
29	0.0	0.0	0.0	0.014620354826791603	6.585745417473695E-4
30	0.0	0.0	0.0	0.027725988207564254	6.585745417473695E-4
31	0.0	0.0	0.0	0.06104986001998115	6.585745417473695E-4
32	0.0	0.0	0.0	0.10043261761647385	6.585745417473695E-4
33	0.0	0.0	0.0	0.13843236867529707	6.585745417473695E-4
34	0.0	0.0	0.0	0.17636626227994556	7.902894500968434E-4
35	0.0	1.317149083494739E-4	0.0	0.2210176162104172	7.902894500968434E-4
36	0.0	1.317149083494739E-4	0.0	0.27910389079253517	8.561469042715804E-4
37	0.0	1.9757236252421084E-4	0.0	0.3717653288163901	8.561469042715804E-4
38	0.0	1.9757236252421084E-4	0.0	0.49406262121887656	8.561469042715804E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2740	0.0	22.64298	1
TAGGACG	115	8.782081E-9	21.041998	4
ACGCGAG	55	0.004483393	20.00057	27
CTATCGG	55	0.004483393	20.00057	1
GGTATCA	1350	0.0	18.741272	1
CCGAACG	60	0.007414556	18.332645	9
CGCAATG	60	0.0074159647	18.332043	2
GTATAGG	140	8.373536E-8	17.286207	1
ATTACAC	270	0.0	17.109907	3
CGAATGC	355	0.0	16.73177	43
TAGCGGC	370	0.0	16.64967	30
AACGCAG	3660	0.0	16.649101	6
TCTAGCG	360	0.0	16.50047	28
CGCAATA	365	0.0	16.275507	36
TATCAAC	3875	0.0	15.952426	2
TACGAAT	360	0.0	15.888294	41
GTATAGT	125	9.440822E-6	15.84045	1
CGGTCCA	375	0.0	15.83993	10
CGCCGGT	350	0.0	15.713181	7
CTAGCGG	380	0.0	15.632023	29
>>END_MODULE