FastQCFastQC Report
Thu 2 Feb 2017
SRR4064218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638306
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27050.42377793722759927No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20210.31661930171422487No Hit
TATCAACGCAGAGTACTTTTTTTTT19790.3100393854984913No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13570.21259395963691397No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10390.16277459400350303No Hit
GTACATGGGGTGGTATCAACGCAAA8980.14068487527925477No Hit
GGTATCAACGCAGAGTACTTTTTTT8980.14068487527925477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC402.7584383E-416.6245881
CCGTCGT755.8285877E-815.1996249
CGTCGTA755.8285877E-815.19962410
CGGTCCA1250.014.43964310
GTTCTAG400.005275457614.2496471
GTCGTAG801.284734E-714.24964711
TAAGACA551.9603196E-413.8156744
TACGCTA655.4439315E-513.1535219
ATACCGT957.3698175E-812.998666
TACCGTC957.3698175E-812.998667
GCGCCGA604.0789045E-412.669332519
ACCGTCG905.3898657E-712.6663548
GGTCGGC604.0870058E-412.66635311
AGGCCCG1207.366907E-1012.66635310
ATACGCT701.0892091E-412.2139848
GCGTCCC550.00306200912.09250516
GCGTTAT550.00306560712.090611
GTATAAT550.00306560712.090611
GGTTCCG550.00306560712.090618
AGAATTT1501.4551915E-1112.03492317
Produced by FastQC (version 0.11.5)