##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064218_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638306
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138853465265875	32.0	32.0	32.0	32.0	32.0
2	30.75280038100848	32.0	32.0	32.0	32.0	32.0
3	30.776369014234554	32.0	32.0	32.0	32.0	32.0
4	30.80483498510119	32.0	32.0	32.0	32.0	32.0
5	30.7333316622435	32.0	32.0	32.0	32.0	32.0
6	34.350709534298595	36.0	36.0	36.0	32.0	36.0
7	34.27210930180822	36.0	36.0	36.0	32.0	36.0
8	34.22251083336206	36.0	36.0	36.0	32.0	36.0
9	34.33793039701961	36.0	36.0	36.0	32.0	36.0
10	34.07037690386743	36.0	36.0	36.0	32.0	36.0
11	34.35013927489323	36.0	36.0	36.0	32.0	36.0
12	34.1756242303848	36.0	36.0	36.0	32.0	36.0
13	34.24555463993759	36.0	36.0	36.0	32.0	36.0
14	34.15060644894455	36.0	36.0	36.0	32.0	36.0
15	34.090202818084116	36.0	36.0	36.0	32.0	36.0
16	34.098360347544904	36.0	36.0	36.0	32.0	36.0
17	34.00682431310375	36.0	36.0	36.0	32.0	36.0
18	34.0083408271268	36.0	36.0	36.0	32.0	36.0
19	34.02127662907759	36.0	36.0	36.0	32.0	36.0
20	33.98814675093137	36.0	36.0	36.0	32.0	36.0
21	33.97480518748061	36.0	36.0	36.0	32.0	36.0
22	33.94592718852713	36.0	36.0	36.0	32.0	36.0
23	33.90890889322676	36.0	36.0	36.0	32.0	36.0
24	33.90505337565369	36.0	36.0	36.0	32.0	36.0
25	33.51199111397982	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	9.0
6	26.0
7	12.0
8	47.0
9	45.0
10	68.0
11	27.0
12	37.0
13	40.0
14	103.0
15	148.0
16	201.0
17	278.0
18	413.0
19	542.0
20	798.0
21	1134.0
22	1783.0
23	2688.0
24	3827.0
25	5534.0
26	7968.0
27	10271.0
28	13918.0
29	18370.0
30	24231.0
31	32237.0
32	44777.0
33	62247.0
34	130841.0
35	275676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.791997743194315	17.26722773363424	11.20174902440171	25.73902549876973
2	16.59146314714195	20.215489418238107	37.5669781345767	25.62606930004324
3	19.03292213306094	24.45973172237245	28.4936095278978	28.013736616668805
4	12.630370104609426	15.326683158932067	35.86159030768676	36.18135642877174
5	14.302143394334419	36.97261218350464	33.55336550513913	15.171878917021811
6	33.59777623523771	35.50173379066339	17.146743090299704	13.753746883799204
7	29.56777103241639	30.810300031027964	21.15355082161427	18.468378114941373
8	27.95480013478462	32.8617438935515	19.831362500097953	19.352093471565933
9	27.565878057994325	14.14291514031466	18.956704114214237	39.33450268747678
10	15.861915444294471	26.99330144740782	32.086270710567035	25.058512397730677
11	36.50771256583898	21.469149736644095	22.96040255831452	19.06273513920241
12	24.65678904734955	24.028450804470484	29.30269818600254	22.012061962177423
13	29.473109918226058	19.893518286258967	25.409496814327998	25.223874981186977
14	23.44974092451928	19.81892152482186	25.9315350908136	30.79980245984526
15	24.830964829083612	27.34552466353653	23.22315150850075	24.600358998879106
16	25.01814704199296	26.029678056581147	24.449827152364602	24.502347749061293
17	23.419275340507188	26.078899841974568	25.804393357915067	24.69743145960318
18	23.934636485221557	25.3697551063286	27.057622685943738	23.6379857225061
19	24.972522949804407	25.022381448875443	26.30615940608807	23.698936195232083
20	25.468508924875504	24.642503230014675	25.87884622621392	24.0101416188959
21	26.277086142556993	24.265577471855497	25.372542255950325	24.08479412963718
22	25.411544088017674	24.528325553755913	25.758058016780055	24.302072341446358
23	24.233277459556845	24.528535610516396	26.28722675292867	24.950960176998088
24	24.400416447153862	25.176864604500636	26.19540402900078	24.22731491934472
25	24.514563106796118	24.787699756654373	26.156832492912873	24.540904643636637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	380.0
1	380.0
2	482.0
3	584.0
4	584.0
5	584.0
6	1606.5
7	2629.0
8	2629.0
9	2629.0
10	2526.5
11	2424.0
12	2424.0
13	2424.0
14	2272.0
15	2120.0
16	2120.0
17	2120.0
18	3586.0
19	5052.0
20	5052.0
21	5052.0
22	7858.5
23	10665.0
24	10665.0
25	10665.0
26	15550.0
27	20435.0
28	20435.0
29	20435.0
30	25300.0
31	30165.0
32	30165.0
33	30165.0
34	35485.0
35	40805.0
36	40805.0
37	40805.0
38	46259.0
39	51713.0
40	51713.0
41	51713.0
42	58881.0
43	66049.0
44	66049.0
45	66049.0
46	73856.5
47	81664.0
48	81664.0
49	81664.0
50	83973.0
51	86282.0
52	86282.0
53	86282.0
54	80075.5
55	73869.0
56	73869.0
57	73869.0
58	67142.0
59	60415.0
60	60415.0
61	60415.0
62	52704.0
63	44993.0
64	44993.0
65	44993.0
66	36781.0
67	28569.0
68	28569.0
69	28569.0
70	21559.5
71	14550.0
72	14550.0
73	14550.0
74	10946.0
75	7342.0
76	7342.0
77	7342.0
78	5809.5
79	4277.0
80	4277.0
81	4277.0
82	2972.0
83	1667.0
84	1667.0
85	1667.0
86	1241.5
87	816.0
88	816.0
89	816.0
90	582.5
91	349.0
92	349.0
93	349.0
94	250.0
95	151.0
96	151.0
97	151.0
98	246.0
99	341.0
100	341.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03697286254555025
2	0.0059532575285208035
3	0.0018799760616381484
4	0.004229946138685835
5	0.010339868339009815
6	0.017076449226546515
7	0.026946323550146794
8	0.03932283262259794
9	0.054362641115703124
10	0.06376252142389387
11	0.060472563316027114
12	0.06783580289077652
13	0.07049910231143057
14	0.07222241370126554
15	0.06595582682913838
16	0.07222241370126554
17	0.0679924675625797
18	0.08130896466584993
19	0.0784890005733927
20	0.08428559343011033
21	0.08240561736847217
22	0.08224895269666899
23	0.08757555153797708
24	0.08303227605568489
25	0.08303227605568489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	638306.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.62222866208364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06532163076294	49.338035574341454
2	12.644748026674051	15.58395108548279
3	3.5330101078310654	6.531358701906561
4	1.329639290110829	3.277413454932003
5	0.6439534203249365	1.984092245749705
6	0.4018589417877646	1.4858066160449155
7	0.2550906507159378	1.1003478085584062
8	0.18320912609455547	0.9031803728943371
9	0.12711068789931512	0.704955948761371
>10	0.6837333926510214	7.825708712438111
>50	0.0753587233202791	3.287474944837016
>100	0.05466692552042717	6.085694222198696
>500	0.001021811691963156	0.4593392586730738
>1k	0.001277264614953945	1.4326410531815092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2705	0.42377793722759927	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2021	0.31661930171422487	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1979	0.3100393854984913	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1357	0.21259395963691397	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1039	0.16277459400350303	No Hit
GTACATGGGGTGGTATCAACGCAAA	898	0.14068487527925477	No Hit
GGTATCAACGCAGAGTACTTTTTTT	898	0.14068487527925477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5666467180317905E-4
2	0.0	0.0	0.0	0.0	1.5666467180317905E-4
3	0.0	0.0	0.0	0.0	1.5666467180317905E-4
4	0.0	0.0	0.0	0.0	1.5666467180317905E-4
5	0.0	0.0	0.0	0.0	1.5666467180317905E-4
6	0.0	0.0	0.0	0.0	3.133293436063581E-4
7	0.0	0.0	0.0	0.0	3.133293436063581E-4
8	0.0	0.0	0.0	0.0	3.133293436063581E-4
9	0.0	0.0	0.0	0.0	3.133293436063581E-4
10	0.0	0.0	0.0	0.0	3.133293436063581E-4
11	0.0	0.0	0.0	0.0	3.133293436063581E-4
12	0.0	0.0	0.0	0.0	4.6999401540953714E-4
13	0.0	0.0	0.0	0.0	4.6999401540953714E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	40	2.7584383E-4	16.624588	1
CCGTCGT	75	5.8285877E-8	15.199624	9
CGTCGTA	75	5.8285877E-8	15.199624	10
CGGTCCA	125	0.0	14.439643	10
GTTCTAG	40	0.0052754576	14.249647	1
GTCGTAG	80	1.284734E-7	14.249647	11
TAAGACA	55	1.9603196E-4	13.815674	4
TACGCTA	65	5.4439315E-5	13.153521	9
ATACCGT	95	7.3698175E-8	12.99866	6
TACCGTC	95	7.3698175E-8	12.99866	7
GCGCCGA	60	4.0789045E-4	12.6693325	19
ACCGTCG	90	5.3898657E-7	12.666354	8
GGTCGGC	60	4.0870058E-4	12.666353	11
AGGCCCG	120	7.366907E-10	12.666353	10
ATACGCT	70	1.0892091E-4	12.213984	8
GCGTCCC	55	0.003062009	12.092505	16
GCGTTAT	55	0.003065607	12.09061	1
GTATAAT	55	0.003065607	12.09061	1
GGTTCCG	55	0.003065607	12.09061	8
AGAATTT	150	1.4551915E-11	12.034923	17
>>END_MODULE