FastQCFastQC Report
Thu 2 Feb 2017
SRR4064218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638306
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.12721171350418137No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.10888194690320945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA300.00574420729.33207911
TATTCCG407.030227E-427.4988255
GTTATAT508.3377985E-526.398871
GTAAACG852.3482244E-723.29494727
TGACCTA602.8715504E-421.999065
GTAAACT500.002579756321.999064
GAATGCG550.004480409420.0007119
TACAGCG550.004482441619.9991477
TTATACT1452.9467628E-1019.7232954
TAGTACA901.0011154E-519.554724
TATACTG808.983052E-519.2491785
TAAACGC1051.7893744E-618.85781528
ATAGTAC708.1199175E-418.8563393
GGCGTTA1303.5810444E-818.6145942
TATAGTA951.5968499E-518.5240732
GTGTATA1701.2551027E-1018.1168731
TATACTA851.4299608E-418.1168735
GTATCAA19450.017.8707031
GTCCTAT2250.017.5992471
GTATAGC1002.480949E-517.5992473
Produced by FastQC (version 0.11.5)