##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638306
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21464156689738	32.0	32.0	32.0	32.0	32.0
2	31.36513051733808	32.0	32.0	32.0	32.0	32.0
3	31.429845559966537	32.0	32.0	32.0	32.0	32.0
4	31.51500377561859	32.0	32.0	32.0	32.0	32.0
5	31.460493869711392	32.0	32.0	32.0	32.0	32.0
6	35.01805090348516	36.0	36.0	36.0	36.0	36.0
7	35.037950450097604	36.0	36.0	36.0	36.0	36.0
8	34.99568388829182	36.0	36.0	36.0	36.0	36.0
9	35.08791551387579	36.0	36.0	36.0	36.0	36.0
10	34.948469856150496	36.0	36.0	36.0	36.0	36.0
11	35.09003048694513	36.0	36.0	36.0	36.0	36.0
12	35.00290456301523	36.0	36.0	36.0	36.0	36.0
13	35.04380657552961	36.0	36.0	36.0	36.0	36.0
14	34.98891440782321	36.0	36.0	36.0	36.0	36.0
15	34.95388888714817	36.0	36.0	36.0	36.0	36.0
16	34.96496194615122	36.0	36.0	36.0	36.0	36.0
17	34.92812538187014	36.0	36.0	36.0	36.0	36.0
18	34.928109715402954	36.0	36.0	36.0	36.0	36.0
19	34.918413738865056	36.0	36.0	36.0	32.0	36.0
20	34.9216927304459	36.0	36.0	36.0	32.0	36.0
21	34.9132046385276	36.0	36.0	36.0	32.0	36.0
22	34.904854411520496	36.0	36.0	36.0	32.0	36.0
23	34.844026219399474	36.0	36.0	36.0	32.0	36.0
24	34.82217619762309	36.0	36.0	36.0	32.0	36.0
25	34.79909635817304	36.0	36.0	36.0	32.0	36.0
26	34.73583986363907	36.0	36.0	36.0	32.0	36.0
27	34.718284020516805	36.0	36.0	36.0	32.0	36.0
28	34.70759478995967	36.0	36.0	36.0	32.0	36.0
29	34.68318643409274	36.0	36.0	36.0	32.0	36.0
30	34.65054221642911	36.0	36.0	36.0	32.0	36.0
31	34.63869993388751	36.0	36.0	36.0	32.0	36.0
32	34.59216582642181	36.0	36.0	36.0	32.0	36.0
33	34.551516044029036	36.0	36.0	36.0	32.0	36.0
34	34.52928376045345	36.0	36.0	36.0	32.0	36.0
35	34.47119250014883	36.0	36.0	36.0	32.0	36.0
36	34.448784752140824	36.0	36.0	36.0	32.0	36.0
37	34.427204193599934	36.0	36.0	36.0	32.0	36.0
38	34.38175577230983	36.0	36.0	36.0	32.0	36.0
39	34.37782035575414	36.0	36.0	36.0	32.0	36.0
40	34.34092425889777	36.0	36.0	36.0	32.0	36.0
41	34.312039993357416	36.0	36.0	36.0	32.0	36.0
42	34.24563767221364	36.0	36.0	36.0	32.0	36.0
43	34.21697900380069	36.0	36.0	36.0	32.0	36.0
44	34.15396690615473	36.0	36.0	36.0	32.0	36.0
45	34.10757536354037	36.0	36.0	36.0	32.0	36.0
46	34.062612602732855	36.0	36.0	36.0	32.0	36.0
47	34.00881238778893	36.0	36.0	36.0	32.0	36.0
48	33.94032485986345	36.0	36.0	36.0	32.0	36.0
49	33.917740080776305	36.0	36.0	36.0	32.0	36.0
50	33.30776931440406	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	8.0
21	25.0
22	77.0
23	285.0
24	687.0
25	1465.0
26	2914.0
27	5428.0
28	9072.0
29	12992.0
30	18259.0
31	25472.0
32	35598.0
33	55426.0
34	110605.0
35	359988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72765360064928	18.25346339812549	11.783544276031499	26.235338725193735
2	15.804975411210107	20.936896344816457	37.00804795244862	26.250080291524817
3	18.297209014499337	24.990404273818942	28.79845841721434	27.91392829446738
4	12.293320131723656	16.001729577976707	35.93871904697747	35.76623124332217
5	13.927645987974419	37.271622074678916	33.344822075932235	15.455909861414433
6	33.6583608149709	35.9229521937005	16.869678288592443	13.549008702736154
7	29.419745388575386	30.888320022058384	21.266132544578934	18.425802044787297
8	27.787926167073472	33.14366463733695	19.723298856661227	19.34511033892835
9	27.54190819363935	13.976030079899735	18.878897070343097	39.60316465611781
10	15.587943619076267	27.121060440137178	32.20236220842518	25.088633732361377
11	36.69014029343339	21.339015047665303	22.853338137724126	19.11750652117718
12	24.536977562485703	24.089543259815823	29.261827399397784	22.11165177830069
13	29.60850881164588	19.736238118887112	25.60805761527049	25.04719545419652
14	23.40178535059987	19.664549604735033	26.16456683784893	30.769098206816164
15	24.79330815063868	27.23491192584945	23.229704090281682	24.74207583323019
16	24.86174256073144	25.969212065761976	24.667007153353744	24.502038220152844
17	23.52696042337061	25.921736596554002	25.99004239346019	24.561260586615198
18	23.996716298190204	25.094782423422068	27.103856469644562	23.80464480874317
19	24.94273906245594	24.93380917616316	26.5178456727651	23.605606088615804
20	25.43161522794924	24.340748864170454	26.071753094156353	24.155882813723956
21	26.096887699629956	24.26516435690719	25.666686510858426	23.971261432604425
22	25.3324037881577	24.390064310948528	26.059955663828422	24.21757623706535
23	24.25736447224742	24.198458094222005	26.540769950227244	25.00340748330333
24	24.453708577730985	24.929970672281545	26.401839875670525	24.21448087431694
25	24.48891346818174	24.550170218565963	26.626476389517734	24.334439923734564
26	23.983530300205402	25.383736798153738	26.843949907796627	23.78878299384423
27	24.484727890890003	24.942851994892322	26.121691173590495	24.45072894062718
28	23.924947240278815	25.03670071176663	26.69602723607997	24.34232481187458
29	23.86962543319275	24.967255955029422	26.57202749873097	24.591091113046858
30	24.02429031818374	25.24569976452304	26.583829328553815	24.14618058873941
31	24.27978509887238	25.20669897390822	25.99839873904617	24.515117188173228
32	24.168584584484215	25.202615157321485	25.876458413953774	24.752341844240526
33	24.038723666935102	24.908893784096286	26.286532574193206	24.76584997477541
34	24.661397338179654	25.07108893362683	26.23780912915077	24.02970459904275
35	24.766676536989426	25.084406926773173	26.222314136338927	23.926602399898478
36	23.967254210732474	25.61707794751273	25.97587152369761	24.439796318057187
37	24.72155680648492	25.550188709988237	25.676152039618916	24.052102443907934
38	24.119459015674256	25.224365603123573	26.123394814522342	24.532780566679826
39	24.557635122220237	25.236497178327806	25.56597950096667	24.63988819848529
40	24.722185230735487	25.55206529998355	25.95831015925488	23.767439310026088
41	23.927668334685922	25.54811196305081	26.416380220654823	24.107839481608444
42	24.693557180726064	25.874834715146044	25.730386719557817	23.70122138457007
43	24.076453078489738	25.308318972270094	26.096663011123294	24.518564938116874
44	23.91950430443604	25.723842423957578	25.584721797914757	24.771931473691634
45	23.996622246174958	25.875693645874787	25.649935609609365	24.47774849834089
46	23.986601869346295	25.604968212339692	25.531021560462353	24.877408357851664
47	24.169345691313694	25.53815495102555	25.841934412463264	24.450564945197495
48	24.652823472642616	25.876244728118163	25.04402373835173	24.42690806088749
49	23.972940750709306	25.98060795578275	25.264333709852533	24.78211758365541
50	24.01848045420933	26.239706969807113	25.170923834709914	24.57088874127364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	149.5
2	60.0
3	163.0
4	266.0
5	288.0
6	310.0
7	283.5
8	257.0
9	370.5
10	484.0
11	686.5
12	889.0
13	1504.5
14	2120.0
15	2663.5
16	3207.0
17	3479.0
18	3751.0
19	3632.0
20	3513.0
21	3791.0
22	4069.0
23	4476.5
24	4884.0
25	5065.5
26	5247.0
27	6179.5
28	7112.0
29	8279.0
30	9446.0
31	10514.5
32	11583.0
33	13292.0
34	15001.0
35	17046.5
36	19092.0
37	21684.5
38	24277.0
39	25913.0
40	27549.0
41	29821.0
42	32093.0
43	33203.0
44	34313.0
45	37256.5
46	40200.0
47	42719.5
48	45239.0
49	45983.5
50	46728.0
51	46021.0
52	45314.0
53	45020.5
54	44727.0
55	44339.5
56	43952.0
57	42514.5
58	41077.0
59	37699.5
60	34322.0
61	30496.5
62	26671.0
63	22899.0
64	19127.0
65	16321.5
66	13516.0
67	11456.0
68	9396.0
69	8183.5
70	6971.0
71	5483.5
72	3996.0
73	3424.0
74	2852.0
75	2233.0
76	1614.0
77	1381.5
78	1149.0
79	964.5
80	780.0
81	609.0
82	438.0
83	350.5
84	263.0
85	184.0
86	105.0
87	75.0
88	45.0
89	32.5
90	20.0
91	16.0
92	12.0
93	10.0
94	8.0
95	10.0
96	12.0
97	9.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009399880308190743
2	0.0010966527026222532
3	1.5666467180317905E-4
4	0.0
5	0.0
6	1.5666467180317905E-4
7	0.0
8	0.0
9	9.399880308190742E-4
10	7.833233590158952E-4
11	1.5666467180317905E-4
12	0.0
13	4.699940154095371E-4
14	0.0
15	0.0057965928567176245
16	6.266586872127162E-4
17	0.0
18	3.133293436063581E-4
19	0.0
20	9.399880308190742E-4
21	0.0
22	1.5666467180317905E-4
23	7.833233590158952E-4
24	3.133293436063581E-4
25	0.0014099820462286114
26	0.0067365808875366985
27	0.00798989826196213
28	0.0054832635131112665
29	0.0034466227796699387
30	0.004229946138685835
31	0.009243215636387563
32	0.004229946138685835
33	0.0062665868721271615
34	0.0032899581078667597
35	0.004543275482292192
36	0.008773221620978025
37	0.003916616795079477
38	0.0097132096517971
39	0.0050132694977017295
40	0.0032899581078667597
41	0.0037599521232762967
42	0.0021933054052445063
43	9.399880308190742E-4
44	0.0017233113898349694
45	0.0018799760616381484
46	0.0012533173744254324
47	0.0021933054052445063
48	0.0021933054052445063
49	4.699940154095371E-4
50	0.0015666467180317904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	638306.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.88770121421445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.50819911799462	53.70387869995127
2	12.035562205061161	15.859910530243235
3	3.1613195095764075	6.248762258689117
4	1.1971047533761339	3.154979212502503
5	0.5916505581513326	1.9491247599349109
6	0.3474707438157475	1.373642912952769
7	0.21817621073329566	1.0062590289391409
8	0.141931515704488	0.7481233039694312
9	0.11303602744807467	0.6702915602646439
>10	0.5770480872525476	7.008687977171142
>50	0.06859007574593591	3.2074005168738715
>100	0.03943321675533734	4.831640259528645
>500	4.779783849008316E-4	0.2372989789793828
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	812	0.12721171350418137	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	695	0.10888194690320945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.5666467180317905E-4
7	0.0	0.0	0.0	0.0	1.5666467180317905E-4
8	0.0	0.0	0.0	0.0	1.5666467180317905E-4
9	0.0	0.0	0.0	0.0	1.5666467180317905E-4
10	0.0	0.0	0.0	0.0	1.5666467180317905E-4
11	0.0	0.0	0.0	0.0	1.5666467180317905E-4
12	0.0	0.0	0.0	0.0	3.133293436063581E-4
13	0.0	0.0	0.0	0.0	3.133293436063581E-4
14	0.0	0.0	0.0	0.0	3.133293436063581E-4
15	0.0	0.0	0.0	1.5666467180317905E-4	6.266586872127162E-4
16	0.0	0.0	0.0	4.6999401540953714E-4	6.266586872127162E-4
17	0.0	0.0	0.0	4.6999401540953714E-4	6.266586872127162E-4
18	0.0	0.0	0.0	4.6999401540953714E-4	6.266586872127162E-4
19	0.0	0.0	0.0	4.6999401540953714E-4	6.266586872127162E-4
20	0.0	0.0	0.0	6.266586872127162E-4	6.266586872127162E-4
21	0.0	0.0	0.0	6.266586872127162E-4	6.266586872127162E-4
22	0.0	0.0	0.0	9.399880308190743E-4	6.266586872127162E-4
23	0.0	0.0	0.0	0.0031332934360635807	6.266586872127162E-4
24	0.0	0.0	0.0	0.004229946138685834	6.266586872127162E-4
25	0.0	0.0	0.0	0.0053265988413080875	6.266586872127162E-4
26	0.0	0.0	0.0	0.0065799162157335195	6.266586872127162E-4
27	0.0	0.0	0.0	0.00814656293376531	6.266586872127162E-4
28	0.0	0.0	0.0	0.015353137836711547	6.266586872127162E-4
29	0.0	0.0	0.0	0.025223012160311826	6.266586872127162E-4
30	0.0	0.0	0.0	0.046059413510134636	6.266586872127162E-4
31	0.0	0.0	0.0	0.0911788389894502	6.266586872127162E-4
32	0.0	0.0	0.0	0.13692492315597848	6.266586872127162E-4
33	0.0	0.0	0.0	0.1817310192916877	6.266586872127162E-4
34	0.0	0.0	0.0	0.23186371426870497	7.833233590158952E-4
35	0.0	0.0	0.0	0.2915529542257162	7.833233590158952E-4
36	0.0	0.0	0.0	0.36800531406566755	7.833233590158952E-4
37	0.0	0.0	0.0	0.48519048857444547	7.833233590158952E-4
38	0.0	0.0	0.0	0.6512550406858153	7.833233590158952E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGTA	30	0.005744207	29.332079	11
TATTCCG	40	7.030227E-4	27.498825	5
GTTATAT	50	8.3377985E-5	26.39887	1
GTAAACG	85	2.3482244E-7	23.294947	27
TGACCTA	60	2.8715504E-4	21.99906	5
GTAAACT	50	0.0025797563	21.99906	4
GAATGCG	55	0.0044804094	20.000711	9
TACAGCG	55	0.0044824416	19.999147	7
TTATACT	145	2.9467628E-10	19.723295	4
TAGTACA	90	1.0011154E-5	19.55472	4
TATACTG	80	8.983052E-5	19.249178	5
TAAACGC	105	1.7893744E-6	18.857815	28
ATAGTAC	70	8.1199175E-4	18.856339	3
GGCGTTA	130	3.5810444E-8	18.61459	42
TATAGTA	95	1.5968499E-5	18.524073	2
GTGTATA	170	1.2551027E-10	18.116873	1
TATACTA	85	1.4299608E-4	18.116873	5
GTATCAA	1945	0.0	17.870703	1
GTCCTAT	225	0.0	17.599247	1
GTATAGC	100	2.480949E-5	17.599247	3
>>END_MODULE