FastQCFastQC Report
Thu 2 Feb 2017
SRR4064217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369878
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT26530.7172635301369641No Hit
GTATCAACGCAGAGTACTTTTTTTT23820.6439961284531657No Hit
TATCAACGCAGAGTACTTTTTTTTT20070.5426113475254003No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18160.4909726991061918No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13110.35444119412346775No Hit
GTACATGGGGTGGTATCAACGCAAA12050.3257830960478861No Hit
GGTATCAACGCAGAGTACTTTTTTT7980.2157468138142847No Hit
GTACATGGGTGGTATCAACGCAAAA6840.18492584041224402No Hit
GTATCAACGCAGAGTACATGGGGTG6630.1792482926802892No Hit
GTACATGGAAGCAGTGGTATCAACG6270.1695153537112237No Hit
GTACATGGGAGTGGTATCAACGCAA5910.1597824147421582No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5650.1527530699311665No Hit
TCCATGTACTCTGCGTTGATACCAC5430.14680516278340425No Hit
TATCAACGCAGAGTACATGGGGTGG4850.13112431666657656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT359.892992E-519.00514213
AACGTCT307.728586E-418.992296
CGAACGT307.728586E-418.992294
GAACGTC307.728586E-418.992295
GTAAGTG307.728586E-418.992295
TCGAACG307.7356223E-418.9897233
TCCTATA250.006042431618.9897212
GGCGTCC453.518939E-516.89346115
CTATACT453.539426E-516.8820364
CGGTCGG402.7511342E-416.6272510
GCGGTCG402.753977E-416.6259
ATTCGAA350.002169912416.2813071
CGGCGTC456.731255E-414.78177814
GTCGGCG456.738169E-414.77977812
GGCGAGA602.5480438E-514.25578919
ACGTCTG400.00526903214.257
CTCCTAA400.00527850314.2461451
CTGTACT400.005283243514.2442184
TAAGTGT400.005283243514.2442186
TGGCGAG954.827598E-914.00568618
Produced by FastQC (version 0.11.5)