##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064217_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369878
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.965104710201743	32.0	32.0	32.0	32.0	32.0
2	30.63192187694321	32.0	32.0	32.0	32.0	32.0
3	30.6707292674882	32.0	32.0	32.0	32.0	32.0
4	30.662607670637346	32.0	32.0	32.0	32.0	32.0
5	30.67088066876105	32.0	32.0	32.0	32.0	32.0
6	34.15248271051536	36.0	36.0	36.0	32.0	36.0
7	34.023991694558745	36.0	36.0	36.0	32.0	36.0
8	33.95704799961068	36.0	36.0	36.0	32.0	36.0
9	34.08061577060544	36.0	36.0	36.0	32.0	36.0
10	33.78812743661423	36.0	36.0	36.0	32.0	36.0
11	34.145664246048696	36.0	36.0	36.0	32.0	36.0
12	33.93902043376465	36.0	36.0	36.0	32.0	36.0
13	34.04558530109928	36.0	36.0	36.0	32.0	36.0
14	33.935189440842656	36.0	36.0	36.0	32.0	36.0
15	33.89483559443925	36.0	36.0	36.0	32.0	36.0
16	33.89834215606227	36.0	36.0	36.0	32.0	36.0
17	33.81251926310838	36.0	36.0	36.0	32.0	36.0
18	33.827459324425895	36.0	36.0	36.0	32.0	36.0
19	33.805503436268175	36.0	36.0	36.0	32.0	36.0
20	33.734396206316674	36.0	36.0	36.0	32.0	36.0
21	33.672408199460364	36.0	36.0	36.0	27.0	36.0
22	33.63203272430369	36.0	36.0	36.0	27.0	36.0
23	33.60753275404323	36.0	36.0	36.0	27.0	36.0
24	33.56107148843673	36.0	36.0	36.0	27.0	36.0
25	32.877492037915204	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	5.0
5	2.0
6	16.0
7	5.0
8	19.0
9	20.0
10	37.0
11	10.0
12	23.0
13	30.0
14	113.0
15	216.0
16	371.0
17	421.0
18	563.0
19	709.0
20	914.0
21	1087.0
22	1365.0
23	1751.0
24	2340.0
25	3185.0
26	4343.0
27	5742.0
28	7649.0
29	10462.0
30	14381.0
31	20526.0
32	31144.0
33	46802.0
34	93748.0
35	121878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.505998680182174	17.116522604584745	11.520278676287633	24.857200038945443
2	15.690033228879113	20.646787018836907	38.71962017958195	24.94355957270203
3	19.178952548064316	25.97108706055387	28.473594705152365	26.376365686229448
4	12.284041074779243	15.435486749111831	35.31749679610233	36.9629753800066
5	13.557301031513202	37.92020981762631	33.649501818329306	14.872987332531196
6	32.69501685923418	35.43251303988081	17.871599423514237	14.000870677370772
7	28.772739195924185	30.776844217409295	21.7814927891701	18.668923797496422
8	28.305765007518634	32.572454752966884	20.141555870484762	18.980224369029717
9	27.853966502207218	14.0079957586774	19.18738639314464	38.95065134597074
10	15.58486176486501	27.747118974192503	32.319969701888226	24.348049559054267
11	36.3473815191517	21.444763038303396	22.955799610473925	19.25205583207098
12	24.542262284792937	24.14918137451169	30.16361688543572	21.144939455259657
13	29.82709596430997	19.753697646546385	26.499146436813326	23.920059952330323
14	23.4122625225235	19.838534254654856	27.081495830785627	29.667707392036014
15	24.337872860767988	27.34889433560039	24.789390815978702	23.52384198765292
16	23.84111335364237	25.965175233075954	26.24086228633577	23.952849126945907
17	22.439726442453363	25.944953036402197	27.14041466476215	24.474905856382286
18	23.18550405445991	25.55594938215821	28.773272221064794	22.485274342317087
19	24.486808676430403	25.208130974380342	27.455972554038294	22.84908779515096
20	24.854024276074053	24.625387600045457	27.269455763538268	23.251132360342226
21	25.846600071429343	24.248909620233984	26.4139763417353	23.49051396660137
22	24.567284927689165	24.681467050881395	26.848762801520625	23.90248521990882
23	23.6166654941582	24.946290093025016	27.248916331599826	24.188128081216956
24	23.60426418463703	25.982575286127872	27.00992992234638	23.403230606888712
25	23.854323092116832	25.35140765994291	27.364205798395496	23.43006344954476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	188.0
1	188.0
2	409.5
3	631.0
4	631.0
5	631.0
6	1861.5
7	3092.0
8	3092.0
9	3092.0
10	2832.0
11	2572.0
12	2572.0
13	2572.0
14	1892.0
15	1212.0
16	1212.0
17	1212.0
18	2114.0
19	3016.0
20	3016.0
21	3016.0
22	4920.0
23	6824.0
24	6824.0
25	6824.0
26	10079.0
27	13334.0
28	13334.0
29	13334.0
30	16610.5
31	19887.0
32	19887.0
33	19887.0
34	21977.5
35	24068.0
36	24068.0
37	24068.0
38	26670.5
39	29273.0
40	29273.0
41	29273.0
42	33813.5
43	38354.0
44	38354.0
45	38354.0
46	44681.0
47	51008.0
48	51008.0
49	51008.0
50	50485.0
51	49962.0
52	49962.0
53	49962.0
54	44976.5
55	39991.0
56	39991.0
57	39991.0
58	36085.5
59	32180.0
60	32180.0
61	32180.0
62	28104.0
63	24028.0
64	24028.0
65	24028.0
66	19484.5
67	14941.0
68	14941.0
69	14941.0
70	11259.5
71	7578.0
72	7578.0
73	7578.0
74	5761.0
75	3944.0
76	3944.0
77	3944.0
78	3103.5
79	2263.0
80	2263.0
81	2263.0
82	1515.0
83	767.0
84	767.0
85	767.0
86	595.0
87	423.0
88	423.0
89	423.0
90	277.0
91	131.0
92	131.0
93	131.0
94	85.5
95	40.0
96	40.0
97	40.0
98	105.5
99	171.0
100	171.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03514672405495866
2	0.005136828900340112
3	0.002433234742266369
4	0.004325750652917989
5	0.008921860721643353
6	0.013788330206176092
7	0.023521269175241565
8	0.03514672405495866
9	0.049205413676942124
10	0.0589383526460076
11	0.05353116432986012
12	0.0632641032989256
13	0.06786021336765095
14	0.07137488577314682
15	0.06380482213054034
16	0.06921201044668783
17	0.0621826656356961
18	0.07705243350510169
19	0.07543027701025744
20	0.0800263870789828
21	0.07624135525767956
22	0.07921530883156068
23	0.0811078247422123
24	0.0778635117525238
25	0.07921530883156068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	369878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.74579620779783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93493488642085	46.958413947212684
2	12.654350374326176	14.867797764644713
3	3.429601006337841	6.044239253771433
4	1.3250325176726538	3.1136036100761197
5	0.661686083836895	1.9435637917309032
6	0.41887314963772093	1.4764222011321577
7	0.25977701390123037	1.068256526267836
8	0.18314386229067967	0.8607145608669806
9	0.12184259597824722	0.6441966281490443
>10	0.8658546750482264	9.776274560126023
>50	0.08372221551749293	3.41310358808242
>100	0.055201460780726	5.55149540854635
>500	0.0032200852122090166	1.2082332654242678
>1k	0.0027600730390363	3.0736848939690495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	2653	0.7172635301369641	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2382	0.6439961284531657	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2007	0.5426113475254003	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1816	0.4909726991061918	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1311	0.35444119412346775	No Hit
GTACATGGGGTGGTATCAACGCAAA	1205	0.3257830960478861	No Hit
GGTATCAACGCAGAGTACTTTTTTT	798	0.2157468138142847	No Hit
GTACATGGGTGGTATCAACGCAAAA	684	0.18492584041224402	No Hit
GTATCAACGCAGAGTACATGGGGTG	663	0.1792482926802892	No Hit
GTACATGGAAGCAGTGGTATCAACG	627	0.1695153537112237	No Hit
GTACATGGGAGTGGTATCAACGCAA	591	0.1597824147421582	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	565	0.1527530699311665	No Hit
TCCATGTACTCTGCGTTGATACCAC	543	0.14680516278340425	No Hit
TATCAACGCAGAGTACATGGGGTGG	485	0.13112431666657656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCGT	35	9.892992E-5	19.005142	13
AACGTCT	30	7.728586E-4	18.99229	6
CGAACGT	30	7.728586E-4	18.99229	4
GAACGTC	30	7.728586E-4	18.99229	5
GTAAGTG	30	7.728586E-4	18.99229	5
TCGAACG	30	7.7356223E-4	18.989723	3
TCCTATA	25	0.0060424316	18.989721	2
GGCGTCC	45	3.518939E-5	16.893461	15
CTATACT	45	3.539426E-5	16.882036	4
CGGTCGG	40	2.7511342E-4	16.62725	10
GCGGTCG	40	2.753977E-4	16.625	9
ATTCGAA	35	0.0021699124	16.281307	1
CGGCGTC	45	6.731255E-4	14.781778	14
GTCGGCG	45	6.738169E-4	14.779778	12
GGCGAGA	60	2.5480438E-5	14.255789	19
ACGTCTG	40	0.005269032	14.25	7
CTCCTAA	40	0.005278503	14.246145	1
CTGTACT	40	0.0052832435	14.244218	4
TAAGTGT	40	0.0052832435	14.244218	6
TGGCGAG	95	4.827598E-9	14.005686	18
>>END_MODULE