FastQCFastQC Report
Thu 2 Feb 2017
SRR4064217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369878
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9620.2600857580066941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.19006266931258414No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA5690.15383450759439599No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.13004287900334704No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA4140.11192879814425297No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.10760304749133497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.10381801567003174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.10138478092776539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCA300.005740614629.33261516
ATAAGAC300.005740614629.3326153
GTCCTAA804.290996E-927.5030421
GGCGTTA651.809325E-523.69172542
TCGAGGG651.809325E-523.69172511
CTACTAG500.002575429622.0024341
CGCGGGA602.868037E-421.9994644
CGTATGT500.002577452721.99945833
CAACGTA500.002577452721.99945830
TAGGGCC500.002577452721.9994584
CGACTTT856.0887196E-620.70817424
GATTAAT856.095852E-620.70537438
CGGCGTT755.4605152E-520.5328341
GCGCCGA755.4605152E-520.5328319
CTAAGAG755.4605152E-520.532832
TTCGGGC755.4605152E-520.5328335
GCATCGA755.4605152E-520.532838
GTAAACG654.919595E-420.30993827
AACGGGT654.924084E-420.30719441
ACTGTTC1950.020.3071928
Produced by FastQC (version 0.11.5)