##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369878
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9472258420344	32.0	32.0	32.0	32.0	32.0
2	31.074300174652183	32.0	32.0	32.0	32.0	32.0
3	31.275928819773007	32.0	32.0	32.0	32.0	32.0
4	31.41929501078734	32.0	32.0	32.0	32.0	32.0
5	31.288441053536573	32.0	32.0	32.0	32.0	32.0
6	34.85267574713824	36.0	36.0	36.0	32.0	36.0
7	34.89384878257155	36.0	36.0	36.0	36.0	36.0
8	34.8260399374929	36.0	36.0	36.0	32.0	36.0
9	34.972136758606894	36.0	36.0	36.0	32.0	36.0
10	34.78954682354723	36.0	36.0	36.0	32.0	36.0
11	34.98943165043609	36.0	36.0	36.0	36.0	36.0
12	34.87053839374064	36.0	36.0	36.0	32.0	36.0
13	34.91479623010831	36.0	36.0	36.0	32.0	36.0
14	34.856577033508344	36.0	36.0	36.0	32.0	36.0
15	34.817220813349266	36.0	36.0	36.0	32.0	36.0
16	34.82038942570253	36.0	36.0	36.0	32.0	36.0
17	34.78333666776613	36.0	36.0	36.0	32.0	36.0
18	34.77091094901562	36.0	36.0	36.0	32.0	36.0
19	34.78030053152661	36.0	36.0	36.0	32.0	36.0
20	34.746219023569935	36.0	36.0	36.0	32.0	36.0
21	34.722513909991946	36.0	36.0	36.0	32.0	36.0
22	34.71992116319435	36.0	36.0	36.0	32.0	36.0
23	34.670802264530465	36.0	36.0	36.0	32.0	36.0
24	34.653861543535974	36.0	36.0	36.0	32.0	36.0
25	34.63371706346417	36.0	36.0	36.0	32.0	36.0
26	34.55390426032368	36.0	36.0	36.0	32.0	36.0
27	34.574286656681394	36.0	36.0	36.0	32.0	36.0
28	34.51997956082816	36.0	36.0	36.0	32.0	36.0
29	34.48691730786908	36.0	36.0	36.0	32.0	36.0
30	34.444381660980106	36.0	36.0	36.0	32.0	36.0
31	34.4256944181595	36.0	36.0	36.0	32.0	36.0
32	34.3523756481867	36.0	36.0	36.0	32.0	36.0
33	34.28817339771492	36.0	36.0	36.0	32.0	36.0
34	34.21914793526514	36.0	36.0	36.0	32.0	36.0
35	34.157265368580994	36.0	36.0	36.0	32.0	36.0
36	34.1090494703659	36.0	36.0	36.0	32.0	36.0
37	34.070688172857	36.0	36.0	36.0	32.0	36.0
38	34.015886319272845	36.0	36.0	36.0	32.0	36.0
39	33.97282076792889	36.0	36.0	36.0	32.0	36.0
40	33.92652982875435	36.0	36.0	36.0	32.0	36.0
41	33.86827818902449	36.0	36.0	36.0	32.0	36.0
42	33.77650738892284	36.0	36.0	36.0	32.0	36.0
43	33.75778770297233	36.0	36.0	36.0	32.0	36.0
44	33.64611033908478	36.0	36.0	36.0	27.0	36.0
45	33.54974072532024	36.0	36.0	36.0	21.0	36.0
46	33.46848420289933	36.0	36.0	36.0	21.0	36.0
47	33.39685247568117	36.0	36.0	36.0	21.0	36.0
48	33.3122083497802	36.0	36.0	36.0	21.0	36.0
49	33.250177085417356	36.0	36.0	36.0	14.0	36.0
50	32.53512779889585	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	14.0
22	47.0
23	124.0
24	402.0
25	926.0
26	1905.0
27	3609.0
28	6144.0
29	9080.0
30	13139.0
31	18456.0
32	26465.0
33	41573.0
34	78164.0
35	169826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.29654864744292	18.283922077570942	12.228035311135445	25.191493963850697
2	15.03584985238296	21.479901372351815	37.723453265418684	25.760795509846545
3	18.666154786172736	26.501981734517866	28.883848187780835	25.94801529152856
4	11.885270278307983	16.11125830679305	35.89724179323993	36.10622962165904
5	13.447947701674606	38.20854443897718	33.07387841396353	15.269629445384695
6	33.19887422034893	35.67104740224453	17.387401757881673	13.742676619524868
7	28.56698695245459	31.23110863582046	21.660926035071025	18.540978376653925
8	28.251747873623195	32.74782495849983	20.129610303938055	18.87081686393892
9	27.85091098836628	13.623865488965132	19.10926182770843	39.41596169496016
10	15.558998150731595	27.805264467010566	32.36057489537034	24.275162486887496
11	36.61126101038721	21.360286364693224	22.980550343626817	19.04790228129275
12	24.29801177685615	24.367764506134453	30.1326383293951	21.201585387614294
13	29.897668131125382	19.930246704967892	26.355931057789796	23.816154106116933
14	23.415081229706093	19.563260218937646	27.270416922382307	29.751241628973958
15	24.364769943709344	27.1725435698334	24.90063969113138	23.562046795325877
16	24.127545447663543	25.900572083617213	26.038185770366283	23.93369669835296
17	22.57744445465802	26.009927597748444	27.102720356441857	24.309907591151678
18	23.520792047085923	25.29381388948218	28.764156733184276	22.421237330247624
19	24.514028950086246	24.956877673178724	27.79484046090873	22.7342529158263
20	24.901588640517364	24.310850122743837	27.617634017887077	23.169927218851726
21	25.86339279438085	24.1457994257566	26.80992110912247	23.18088667074008
22	24.574926259269976	24.67225591210051	27.088734903765303	23.66408292486421
23	23.72152754308888	24.65184183845894	27.491449814126394	24.13518080432579
24	23.716532793334	25.703409511810683	27.21823741405927	23.361820280796046
25	24.143347662692296	24.747073296022926	27.59996755616838	23.50961148511639
26	23.090420944197653	25.708716024214635	28.1904725854988	23.010390446088916
27	23.640473377260697	25.421991980597696	27.689108916245104	23.248425725896503
28	22.858193439192352	25.721686137840226	28.04714177952377	23.372978643443652
29	23.006129163951755	25.299090764077203	27.85295413806435	23.841825933906684
30	23.157203150389744	25.7903687621499	27.75706417851127	23.295363908949085
31	23.13721779099635	25.71177504393673	27.27754495065567	23.873462214411248
32	23.105499378143083	26.124749905369598	26.922078624344348	23.847672092142975
33	23.068749594445524	25.618876127441435	27.44251941254083	23.86985486557221
34	23.507034942195595	25.886532492673066	27.432489428661032	23.17394313647031
35	23.83701124190923	26.081067197309295	27.194713761200003	22.887207799581468
36	23.196368267221125	26.703726635861468	26.708323081457124	23.39158201546028
37	23.61327406830619	26.531914433413363	26.675210347587218	23.179601150693227
38	23.45131905896222	26.643449858995748	26.649939028841658	23.255292053200378
39	23.58439297137558	26.181598933647688	26.763983031371414	23.47002506360532
40	24.204786018833698	26.9181791391006	26.189813150061237	22.687221692004467
41	22.839167380883502	26.702967807521038	27.32318739878173	23.134677412813726
42	24.2284472153805	27.438214945210625	25.955806213517686	22.37753162589119
43	23.41337861001314	26.475502468408163	26.502809064708522	23.60830985687018
44	22.973144690986857	27.15054708263151	26.12938024338215	23.746927982999477
45	23.29624873267996	27.135924298749575	25.777627576884083	23.790199391686382
46	23.10050692801622	26.63413315309226	25.88712402838797	24.37823589050355
47	23.681627819579365	27.003614793345754	25.689095328345985	23.625662058728903
48	23.91961456519623	27.297576432673274	24.98810386408124	23.79470513804925
49	23.454076501313953	27.15802052579783	25.499897262866472	23.888005710021737
50	23.392560709863357	27.573714291893996	24.79384871604925	24.239876282193396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	40.5
2	36.0
3	122.0
4	208.0
5	252.5
6	297.0
7	286.0
8	275.0
9	367.0
10	459.0
11	650.5
12	842.0
13	1373.0
14	1904.0
15	2523.0
16	3142.0
17	3373.0
18	3604.0
19	3652.5
20	3701.0
21	3822.5
22	3944.0
23	4132.5
24	4321.0
25	4086.5
26	3852.0
27	4430.5
28	5009.0
29	5519.5
30	6030.0
31	6786.0
32	7542.0
33	8819.5
34	10097.0
35	11403.5
36	12710.0
37	13610.5
38	14511.0
39	15214.5
40	15918.0
41	17043.5
42	18169.0
43	18652.0
44	19135.0
45	20755.5
46	22376.0
47	24198.0
48	26020.0
49	26516.5
50	27013.0
51	26038.0
52	25063.0
53	24379.0
54	23695.0
55	23636.5
56	23578.0
57	22802.5
58	22027.0
59	20160.0
60	18293.0
61	16175.5
62	14058.0
63	12124.5
64	10191.0
65	8662.0
66	7133.0
67	6050.0
68	4967.0
69	4389.0
70	3811.0
71	2998.5
72	2186.0
73	1808.5
74	1431.0
75	1111.5
76	792.0
77	687.0
78	582.0
79	493.0
80	404.0
81	311.0
82	218.0
83	172.5
84	127.0
85	100.0
86	73.0
87	56.5
88	40.0
89	28.0
90	16.0
91	15.0
92	14.0
93	11.5
94	9.0
95	5.5
96	2.0
97	4.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0062182665635696095
2	5.407188316147487E-4
3	0.0
4	0.0
5	0.0
6	2.7035941580737434E-4
7	0.0
8	0.0
9	0.0013517970790368715
10	5.407188316147487E-4
11	0.0
12	0.0
13	8.11078247422123E-4
14	2.7035941580737434E-4
15	0.003244312989688492
16	5.407188316147487E-4
17	0.0
18	2.7035941580737434E-4
19	0.0
20	5.407188316147487E-4
21	0.0
22	2.7035941580737434E-4
23	8.11078247422123E-4
24	2.7035941580737434E-4
25	0.0021628753264589947
26	0.005136828900340112
27	0.006758985395184359
28	0.004055391237110615
29	0.0018925159106516203
30	0.005136828900340112
31	0.007570063642606481
32	0.004866469484532738
33	0.0037850318213032406
34	0.002703594158073743
35	0.003244312989688492
36	0.007299704226799106
37	0.0037850318213032406
38	0.008381141890028605
39	0.005136828900340112
40	0.002433234742266369
41	0.002433234742266369
42	0.0018925159106516203
43	0.0010814376632294973
44	0.0018925159106516203
45	8.11078247422123E-4
46	8.11078247422123E-4
47	0.002433234742266369
48	0.002703594158073743
49	5.407188316147487E-4
50	0.0010814376632294973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	369878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.39237011128488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1705011757526	53.73323825114582
2	11.336396327571205	14.826276487614937
3	3.065686219037238	6.014174636410458
4	1.1998252329419499	3.1383766280559424
5	0.5642899547967165	1.8450128787073548
6	0.3373628286800528	1.32365729729016
7	0.22739030441300614	1.0408713662125122
8	0.1596055464528424	0.834958797236651
9	0.13301042619187897	0.7828080316379179
>10	0.6965252924421911	8.601124406920322
>50	0.07459547162800491	3.4056393289121925
>100	0.03356796223191447	3.8484510312042106
>500	0.0012432578604412766	0.6054108586515179
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	962	0.2600857580066941	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	0.19006266931258414	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	569	0.15383450759439599	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	481	0.13004287900334704	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	414	0.11192879814425297	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	398	0.10760304749133497	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.10381801567003174	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.10138478092776539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.7035941580737434E-4
13	0.0	0.0	0.0	0.0	8.11078247422123E-4
14	0.0	0.0	0.0	0.0	8.11078247422123E-4
15	0.0	0.0	0.0	0.0	8.11078247422123E-4
16	0.0	0.0	0.0	2.7035941580737434E-4	8.11078247422123E-4
17	0.0	0.0	0.0	2.7035941580737434E-4	8.11078247422123E-4
18	0.0	0.0	0.0	2.7035941580737434E-4	8.11078247422123E-4
19	0.0	0.0	0.0	2.7035941580737434E-4	8.11078247422123E-4
20	0.0	0.0	0.0	2.7035941580737434E-4	0.001622156494844246
21	0.0	0.0	0.0	2.7035941580737434E-4	0.001622156494844246
22	0.0	0.0	0.0	0.0013517970790368715	0.001622156494844246
23	0.0	0.0	0.0	0.003244312989688492	0.001622156494844246
24	0.0	0.0	0.0	0.006488625979376984	0.001622156494844246
25	0.0	0.0	0.0	0.009462579553258101	0.001622156494844246
26	0.0	0.0	0.0	0.011084736048102348	0.001622156494844246
27	0.0	0.0	0.0	0.012436533127139219	0.001622156494844246
28	0.0	0.0	0.0	0.01757336202747933	0.001622156494844246
29	0.0	0.0	0.0	0.024061988006856314	0.001622156494844246
30	0.0	0.0	0.0	0.04325750652917989	0.001622156494844246
31	0.0	0.0	0.0	0.07597099584187218	0.0018925159106516203
32	0.0	0.0	0.0	0.10381801567003174	0.0018925159106516203
33	0.0	0.0	0.0	0.13193539491399867	0.0018925159106516203
34	0.0	0.0	0.0	0.1665414001373426	0.0021628753264589947
35	0.0	0.0	0.0	0.20790639075587086	0.0021628753264589947
36	0.0	0.0	0.0	0.25413785085893187	0.0021628753264589947
37	0.0	0.0	0.0	0.33010884670080404	0.0021628753264589947
38	0.0	0.0	0.0	0.4390636912711759	0.0021628753264589947
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCA	30	0.0057406146	29.332615	16
ATAAGAC	30	0.0057406146	29.332615	3
GTCCTAA	80	4.290996E-9	27.503042	1
GGCGTTA	65	1.809325E-5	23.691725	42
TCGAGGG	65	1.809325E-5	23.691725	11
CTACTAG	50	0.0025754296	22.002434	1
CGCGGGA	60	2.868037E-4	21.99946	44
CGTATGT	50	0.0025774527	21.999458	33
CAACGTA	50	0.0025774527	21.999458	30
TAGGGCC	50	0.0025774527	21.999458	4
CGACTTT	85	6.0887196E-6	20.708174	24
GATTAAT	85	6.095852E-6	20.705374	38
CGGCGTT	75	5.4605152E-5	20.53283	41
GCGCCGA	75	5.4605152E-5	20.53283	19
CTAAGAG	75	5.4605152E-5	20.53283	2
TTCGGGC	75	5.4605152E-5	20.53283	35
GCATCGA	75	5.4605152E-5	20.53283	8
GTAAACG	65	4.919595E-4	20.309938	27
AACGGGT	65	4.924084E-4	20.307194	41
ACTGTTC	195	0.0	20.307192	8
>>END_MODULE