FastQCFastQC Report
Thu 2 Feb 2017
SRR4064216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450775
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89110.614223432303424No Hit
TATCAACGCAGAGTACTTTTTTTTT55310.3812445072461271No Hit
GTCCTACAGTGGACATTTCTAAATT41110.28336578725164135No Hit
GTCCTAAAGTGTGTATTTCTCATTT37950.2615843256190657No Hit
CTGTAGGACGTGGAATATGGCAAGA37200.2564146749151316No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31910.21995140528338303No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30030.20699281418552154No Hit
GGTATCAACGCAGAGTACTTTTTTT29930.206303527424997No Hit
CTTTAGGACGTGAAATATGGCGAGG28250.1947235098481846No Hit
GTCCTACAGTGTGCATTTCTCATTT27240.18776171356688665No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25480.17563026658165465No Hit
CTGTAGGACCTGGAATATGGCGAGA18890.13020626906308697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTCG508.707898E-515.20110518
GGGCGTA704.428257E-714.93068516
TCCGTCG653.3704127E-614.6159428
CGAATGA2150.014.58346114
TCGCTTA551.9599486E-413.81823211
TGCGAAT2300.013.63189512
CCGTCGC707.258881E-613.5719469
GCGAATG2250.013.51255813
CGCACGA500.001499031413.30050810
ACTCGGT1301.4551915E-1113.15570914
CGCGGGC905.397105E-712.66802313
CGTCGCG751.4778418E-512.6671510
ACTGTGC2400.012.6671498
GCACGAA604.0916068E-412.66671311
ATCCGTC905.40389E-712.6667137
CTTATAC2750.012.43254853
TCGGTGA1301.9645086E-1012.42483716
AGCGAAA1301.9826984E-1012.42312211
TAGGACC8650.012.4087384
GTATCAA49400.012.4013241
Produced by FastQC (version 0.11.5)