##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064216_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1450775
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222867777567163	32.0	32.0	32.0	32.0	32.0
2	30.975967327807552	32.0	32.0	32.0	32.0	32.0
3	30.97830814564629	32.0	32.0	32.0	32.0	32.0
4	30.985280281229	32.0	32.0	32.0	32.0	32.0
5	31.004797435853252	32.0	32.0	32.0	32.0	32.0
6	34.618038634522925	36.0	36.0	36.0	32.0	36.0
7	34.5245747962296	36.0	36.0	36.0	32.0	36.0
8	34.4976409160621	36.0	36.0	36.0	32.0	36.0
9	34.55406386241836	36.0	36.0	36.0	32.0	36.0
10	34.40019575743999	36.0	36.0	36.0	32.0	36.0
11	34.58768244558943	36.0	36.0	36.0	32.0	36.0
12	34.45825024555841	36.0	36.0	36.0	32.0	36.0
13	34.493923592562595	36.0	36.0	36.0	32.0	36.0
14	34.44250831452155	36.0	36.0	36.0	32.0	36.0
15	34.420111319811824	36.0	36.0	36.0	32.0	36.0
16	34.42188830108046	36.0	36.0	36.0	32.0	36.0
17	34.36994227223381	36.0	36.0	36.0	32.0	36.0
18	34.36444107459806	36.0	36.0	36.0	32.0	36.0
19	34.33370371008599	36.0	36.0	36.0	32.0	36.0
20	34.307867863728006	36.0	36.0	36.0	32.0	36.0
21	34.28564112284813	36.0	36.0	36.0	32.0	36.0
22	34.274210680498356	36.0	36.0	36.0	32.0	36.0
23	34.258037255949404	36.0	36.0	36.0	32.0	36.0
24	34.25049576950251	36.0	36.0	36.0	32.0	36.0
25	33.88501180403578	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	10.0
5	22.0
6	49.0
7	12.0
8	75.0
9	95.0
10	117.0
11	39.0
12	74.0
13	83.0
14	158.0
15	287.0
16	458.0
17	555.0
18	648.0
19	891.0
20	1229.0
21	1698.0
22	2662.0
23	4073.0
24	6402.0
25	9479.0
26	13739.0
27	18570.0
28	25649.0
29	35324.0
30	47130.0
31	64432.0
32	90836.0
33	130562.0
34	290323.0
35	705090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.354207004595075	19.4377099640889	12.219427297951798	23.988655733364226
2	13.441276401854823	21.261347084827147	43.99956434914659	21.29781216417144
3	18.445825644786733	27.76479216626423	29.29889415895629	24.49048802999274
4	10.491284375844403	16.990504077952586	40.356014359686995	32.16219718651601
5	11.217315716512289	40.478499771483165	35.124795006958905	13.179389505045638
6	28.44237816731881	39.534853260315074	19.829849248171097	12.192919324195021
7	27.176570353057972	33.388281039938775	21.979082642178053	17.456065964825196
8	25.217938118881854	37.301463079631276	20.515659895869604	16.964938905617263
9	28.159572450650806	14.78782863546246	20.672355831393848	36.38024308249289
10	14.596616626654427	30.164216850934466	34.13353214163298	21.105634380778124
11	33.38331055162987	23.410303766774206	25.364035989114438	17.842349692481484
12	23.86339653353013	26.36648297457048	32.13071336446213	17.639407127437252
13	28.519148202431243	23.574308130011207	26.71685490128459	21.189688766272955
14	20.586571833737054	23.413419889533245	28.919238430511335	27.08076984621836
15	21.93160022263343	33.104377698861384	24.751416805237817	20.212605273267368
16	20.359461098040676	29.73065248024433	29.64933299398484	20.26055342773015
17	19.695149287187306	30.164150383822218	28.67727896599052	21.463421362999952
18	19.22472021720416	28.692498289296513	32.75348762775092	19.329293865748408
19	22.943178878752867	26.720998810119163	29.54125782475987	20.794564486368106
20	23.613808079477234	27.306640876942595	30.162664269804296	18.91688677377588
21	23.559267552082687	26.237657910456818	28.071329931282996	22.131744606177495
22	22.439088659705632	29.603935740700248	28.659457304290783	19.297518295303338
23	20.561350452903362	29.571061092803973	29.86382202011938	20.003766434173283
24	21.367026123570124	27.78116931447961	30.767469115747055	20.084335446203212
25	20.48987464104073	28.69894466146597	30.561014626016693	20.25016607147661
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	163.0
1	163.0
2	511.0
3	859.0
4	859.0
5	859.0
6	2607.5
7	4356.0
8	4356.0
9	4356.0
10	5016.0
11	5676.0
12	5676.0
13	5676.0
14	6698.5
15	7721.0
16	7721.0
17	7721.0
18	13780.0
19	19839.0
20	19839.0
21	19839.0
22	32020.5
23	44202.0
24	44202.0
25	44202.0
26	64297.0
27	84392.0
28	84392.0
29	84392.0
30	108959.0
31	133526.0
32	133526.0
33	133526.0
34	147953.5
35	162381.0
36	162381.0
37	162381.0
38	170144.5
39	177908.0
40	177908.0
41	177908.0
42	184957.0
43	192006.0
44	192006.0
45	192006.0
46	193756.5
47	195507.0
48	195507.0
49	195507.0
50	182677.0
51	169847.0
52	169847.0
53	169847.0
54	140135.0
55	110423.0
56	110423.0
57	110423.0
58	89378.0
59	68333.0
60	68333.0
61	68333.0
62	53260.5
63	38188.0
64	38188.0
65	38188.0
66	29321.5
67	20455.0
68	20455.0
69	20455.0
70	14273.5
71	8092.0
72	8092.0
73	8092.0
74	5766.0
75	3440.0
76	3440.0
77	3440.0
78	2514.5
79	1589.0
80	1589.0
81	1589.0
82	1159.0
83	729.0
84	729.0
85	729.0
86	539.0
87	349.0
88	349.0
89	349.0
90	229.0
91	109.0
92	109.0
93	109.0
94	89.5
95	70.0
96	70.0
97	70.0
98	342.5
99	615.0
100	615.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03632541227964364
2	0.00496286467577674
3	0.0016542882252589134
4	0.003239647774465372
5	0.007995726422084747
6	0.012338233013389394
7	0.02102324619599869
8	0.03294790715307336
9	0.04480363943409557
10	0.055280798194068684
11	0.05383329599696714
12	0.06100187830642243
13	0.062035808447209254
14	0.06389688270062553
15	0.059140804053006146
16	0.06465509813720252
17	0.06100187830642243
18	0.07437404146059864
19	0.07237510985507746
20	0.0759594010098051
21	0.07313332529165446
22	0.07485654219296582
23	0.07775154658716893
24	0.07499439954507074
25	0.07568368630559529
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1450775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.40785784801167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78307852980096	42.54385900311929
2	14.328225158698551	15.877925256149478
3	4.3615599444891	7.249940801995014
4	1.7287550559854925	3.8314645758430252
5	0.8356462374099286	2.3150683966817573
6	0.45622757025255745	1.5167155415338478
7	0.2935167251841911	1.1384193089513697
8	0.19319080819185266	0.8563431070269331
9	0.1339753009169426	0.6680955985515489
>10	0.731139687153625	7.615546735930192
>50	0.08425610052642471	3.2540021874265967
>100	0.0609553485298076	6.748009564623282
>500	0.006855846149064178	2.511961822884204
>1k	0.0023683832151312613	2.8751844576531904
>5k	2.4930349632960646E-4	0.9974636416302909
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8911	0.614223432303424	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5531	0.3812445072461271	No Hit
GTCCTACAGTGGACATTTCTAAATT	4111	0.28336578725164135	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3795	0.2615843256190657	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3720	0.2564146749151316	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3191	0.21995140528338303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3003	0.20699281418552154	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2993	0.206303527424997	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2825	0.1947235098481846	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2724	0.18776171356688665	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2548	0.17563026658165465	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1889	0.13020626906308697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.892867605245473E-5	0.0
12	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
13	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCG	50	8.707898E-5	15.201105	18
GGGCGTA	70	4.428257E-7	14.930685	16
TCCGTCG	65	3.3704127E-6	14.615942	8
CGAATGA	215	0.0	14.583461	14
TCGCTTA	55	1.9599486E-4	13.818232	11
TGCGAAT	230	0.0	13.631895	12
CCGTCGC	70	7.258881E-6	13.571946	9
GCGAATG	225	0.0	13.512558	13
CGCACGA	50	0.0014990314	13.300508	10
ACTCGGT	130	1.4551915E-11	13.155709	14
CGCGGGC	90	5.397105E-7	12.668023	13
CGTCGCG	75	1.4778418E-5	12.66715	10
ACTGTGC	240	0.0	12.667149	8
GCACGAA	60	4.0916068E-4	12.666713	11
ATCCGTC	90	5.40389E-7	12.666713	7
CTTATAC	275	0.0	12.4325485	3
TCGGTGA	130	1.9645086E-10	12.424837	16
AGCGAAA	130	1.9826984E-10	12.423122	11
TAGGACC	865	0.0	12.408738	4
GTATCAA	4940	0.0	12.401324	1
>>END_MODULE