FastQCFastQC Report
Thu 2 Feb 2017
SRR4064216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450775
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.13723699402043735No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19740.1360652065275456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT1900.026.63095325
TAGGACC11600.026.5511024
TTACGGC1750.026.3993818
GGACCGT2150.024.5575626
TAGCGCG450.001398539624.4438717
CCGCCGT2100.024.09467324
TGTGCGA1750.023.88762331
GCCGTTT2150.023.5343326
GTATCAA58350.023.4133321
AGGCGTA852.3538632E-723.2935718
TGTTACG952.6870111E-823.1573516
TGCGAAG1800.022.00251633
ATAGCGC602.873618E-421.99948316
GTCGGCA703.2165095E-521.99948316
GACCGTT2300.021.9994817
GGCCGCC2200.021.99948122
TACGGCC2100.021.99872619
CCTACCG803.61819E-621.9987243
GTAGGAC25050.021.6913573
ACCGTTA2250.021.5106058
Produced by FastQC (version 0.11.5)