##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064216_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1450775
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27231376333339	32.0	32.0	32.0	32.0	32.0
2	31.445987144801915	32.0	32.0	32.0	32.0	32.0
3	31.500072719753234	32.0	32.0	32.0	32.0	32.0
4	31.568546466543744	32.0	32.0	32.0	32.0	32.0
5	31.541081835570644	32.0	32.0	32.0	32.0	32.0
6	35.11539073943237	36.0	36.0	36.0	36.0	36.0
7	35.13426203236202	36.0	36.0	36.0	36.0	36.0
8	35.08827902328066	36.0	36.0	36.0	36.0	36.0
9	35.18350605710741	36.0	36.0	36.0	36.0	36.0
10	35.06981647740001	36.0	36.0	36.0	36.0	36.0
11	35.186367975737106	36.0	36.0	36.0	36.0	36.0
12	35.126303527424994	36.0	36.0	36.0	36.0	36.0
13	35.14605228240079	36.0	36.0	36.0	36.0	36.0
14	35.10159259706019	36.0	36.0	36.0	36.0	36.0
15	35.08835829125812	36.0	36.0	36.0	36.0	36.0
16	35.10705657321087	36.0	36.0	36.0	36.0	36.0
17	35.09021109407041	36.0	36.0	36.0	36.0	36.0
18	35.069665523599454	36.0	36.0	36.0	36.0	36.0
19	35.063072495735035	36.0	36.0	36.0	36.0	36.0
20	35.05294825179645	36.0	36.0	36.0	36.0	36.0
21	35.03958987437749	36.0	36.0	36.0	36.0	36.0
22	35.02354534645276	36.0	36.0	36.0	36.0	36.0
23	34.99171063741793	36.0	36.0	36.0	36.0	36.0
24	34.98289810618463	36.0	36.0	36.0	36.0	36.0
25	34.954305112784546	36.0	36.0	36.0	36.0	36.0
26	34.91770605366097	36.0	36.0	36.0	36.0	36.0
27	34.88533507952646	36.0	36.0	36.0	36.0	36.0
28	34.86656235460357	36.0	36.0	36.0	32.0	36.0
29	34.84757595078492	36.0	36.0	36.0	32.0	36.0
30	34.816511864348364	36.0	36.0	36.0	32.0	36.0
31	34.792251038238184	36.0	36.0	36.0	32.0	36.0
32	34.759927624890146	36.0	36.0	36.0	32.0	36.0
33	34.75640536954386	36.0	36.0	36.0	32.0	36.0
34	34.7395233582051	36.0	36.0	36.0	32.0	36.0
35	34.70748599886268	36.0	36.0	36.0	32.0	36.0
36	34.67250469576606	36.0	36.0	36.0	32.0	36.0
37	34.62613430752529	36.0	36.0	36.0	32.0	36.0
38	34.59919904878427	36.0	36.0	36.0	32.0	36.0
39	34.539302097154966	36.0	36.0	36.0	32.0	36.0
40	34.54317588874912	36.0	36.0	36.0	32.0	36.0
41	34.51478003136255	36.0	36.0	36.0	32.0	36.0
42	34.482359428581276	36.0	36.0	36.0	32.0	36.0
43	34.453408695352486	36.0	36.0	36.0	32.0	36.0
44	34.386983508814254	36.0	36.0	36.0	32.0	36.0
45	34.32861953094036	36.0	36.0	36.0	32.0	36.0
46	34.23341731143699	36.0	36.0	36.0	32.0	36.0
47	34.19558787544588	36.0	36.0	36.0	32.0	36.0
48	34.13686960417708	36.0	36.0	36.0	32.0	36.0
49	34.090745291309815	36.0	36.0	36.0	32.0	36.0
50	33.58020368423774	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	13.0
21	42.0
22	153.0
23	497.0
24	1139.0
25	2468.0
26	5015.0
27	9570.0
28	16040.0
29	24967.0
30	36137.0
31	51568.0
32	74199.0
33	115845.0
34	234609.0
35	878505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34205169248671	20.468945283282906	12.695563336152823	24.493439688077558
2	12.993963189101482	22.057588887525625	43.19580139043788	21.752646532935017
3	17.871393900208922	28.235744993524133	29.731846032213216	24.161015074053726
4	10.344884630628458	17.751477658492874	40.16322310489222	31.740414605986455
5	10.974203442987369	41.041236580448384	34.81035998001069	13.174199996553565
6	28.777968710473655	39.82325284520735	19.360437504695774	12.038340939623222
7	27.1954330553436	33.447892950861366	21.898808428334412	17.45786556546062
8	25.44274611845393	37.58708276610777	20.196033154693183	16.77413796074512
9	27.948691790934692	14.842269097205468	20.645770981043064	36.56326813081677
10	14.545765354548895	30.51981755756814	33.89756672197105	21.036850365911913
11	33.4881701159725	23.39825265806207	25.31019627440506	17.803380951560374
12	23.86186379518201	26.512867985367784	31.99464284852675	17.63062537092346
13	28.53872670287275	23.62167926113668	26.6747497361744	21.164844299816167
14	20.667809505691103	23.599970498485977	28.670508756366438	27.061711239456482
15	22.09077655346883	33.1611871469389	24.496020900183012	20.252015399409252
16	20.508409034982332	29.81207793702753	29.351078502937423	20.328434525052714
17	19.859661215557203	30.336130688769796	28.458168909720666	21.346039185952336
18	19.40944382561512	28.851821931556255	32.372026402567464	19.366707840261157
19	23.203735934242044	26.76259240750633	29.35100205062811	20.68266960762351
20	23.921434824265734	27.45872881297518	29.771638509205456	18.848197853553632
21	23.724147438438077	26.30304492426462	27.899019489583154	22.07378814771415
22	22.502088543203204	29.82667159277957	28.372549235855114	19.29869062816211
23	20.747206299577325	29.755494346427124	29.489082993512383	20.008216360483168
24	21.61012538867318	27.99349862038684	30.31385423868307	20.082521752256906
25	20.708805790108563	28.7400999483026	30.11173530932276	20.43935895226607
26	19.514954077595213	29.376621621994225	30.482357579885132	20.626066720525426
27	20.672885703315693	29.456051428717174	29.310398309238185	20.56066455872895
28	19.516573081339963	29.803698286237374	29.09880983728147	21.58091879514119
29	21.12183353437877	28.730863346544886	28.585972772703773	21.561330346372564
30	19.920233041939	29.108804832890566	30.222178412739552	20.74878371243088
31	21.881419491817518	27.900335019921968	29.547929907766118	20.670315580494396
32	20.318858841305378	29.62641031862448	29.387703896504945	20.667026943565197
33	19.75478163405843	29.545318248274803	30.234981977752916	20.46491813991385
34	20.309756808714933	29.465635384707646	30.507440629025933	19.717167177551488
35	20.327090724732187	29.7398066765054	29.864985121273513	20.068117477488904
36	19.79201205519415	29.25369883108459	30.69138827058837	20.26290084313289
37	20.163792384433037	28.863841118216303	28.708058444696118	22.264308052654545
38	20.23691350671696	30.330691769792423	28.989091831557584	20.44330289193303
39	21.869821522613663	28.596293140766356	29.062614598584425	20.471270738035564
40	22.026589317262378	28.439071834720792	29.832850014440975	19.70148883357585
41	22.071891982805923	27.814854845691695	29.924893088454656	20.18836008304773
42	20.693270432404823	29.767216475374408	29.50045735059249	20.039055741628278
43	19.951418365898007	29.566418565794063	30.066641208640473	20.415521859667457
44	19.537277013427445	29.566572003639475	29.745168049849735	21.15098293308335
45	20.11832443338202	29.83070217824212	29.095982304410732	20.95499108396513
46	20.499559540598778	28.549351442487307	29.405179912845558	21.545909104068357
47	21.4116442000954	28.663903034848232	28.82395839939121	21.10049436566516
48	19.666063072073605	29.39225354005816	29.302713832058245	21.638969555809982
49	21.233635403017296	28.506261162183645	28.732003509862704	21.528099924936363
50	19.992693484794177	30.3771126857647	28.412211530508145	21.217982298932974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	24.5
2	28.0
3	114.5
4	201.0
5	296.0
6	391.0
7	406.0
8	421.0
9	591.0
10	761.0
11	1251.0
12	1741.0
13	3017.0
14	4293.0
15	5978.0
16	7663.0
17	8151.5
18	8640.0
19	9056.5
20	9473.0
21	10701.5
22	11930.0
23	14081.5
24	16233.0
25	18611.5
26	20990.0
27	26348.5
28	31707.0
29	39587.5
30	47468.0
31	57466.5
32	67465.0
33	80416.0
34	93367.0
35	105659.0
36	117951.0
37	121408.0
38	124865.0
39	120555.5
40	116246.0
41	111944.5
42	107643.0
43	103317.0
44	98991.0
45	96121.5
46	93252.0
47	91102.0
48	88952.0
49	83614.5
50	78277.0
51	76597.5
52	74918.0
53	68060.0
54	61202.0
55	55202.0
56	49202.0
57	43597.0
58	37992.0
59	33041.5
60	28091.0
61	23428.5
62	18766.0
63	14960.0
64	11154.0
65	9302.5
66	7451.0
67	5987.5
68	4524.0
69	3684.5
70	2845.0
71	2461.5
72	2078.0
73	1774.0
74	1470.0
75	1210.0
76	950.0
77	717.5
78	485.0
79	366.0
80	247.0
81	209.0
82	171.0
83	143.5
84	116.0
85	86.0
86	56.0
87	40.0
88	24.0
89	23.5
90	23.0
91	18.5
92	14.0
93	11.0
94	8.0
95	7.0
96	6.0
97	6.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010890730816287848
2	6.203580844720925E-4
3	2.757147042098189E-4
4	0.0
5	0.0
6	1.3785735210490946E-4
7	1.3785735210490946E-4
8	0.0
9	8.960727886819114E-4
10	0.0011028588168392757
11	0.0
12	2.757147042098189E-4
13	4.1357205631472834E-4
14	1.3785735210490946E-4
15	0.004825007323671831
16	9.650014647343662E-4
17	0.0
18	4.825007323671831E-4
19	0.0
20	3.446433802622736E-4
21	0.0
22	2.0678602815736417E-4
23	7.582154365770019E-4
24	4.1357205631472834E-4
25	0.0017232169013113683
26	0.004618221295514466
27	0.004687149971566921
28	0.003377505126570281
29	0.0017232169013113683
30	0.003377505126570281
31	0.007926797746032293
32	0.002688218366045734
33	0.004687149971566921
34	0.0016542882252589134
35	0.0031707190984129174
36	0.007857869069979838
37	0.00248143233788837
38	0.008202512450242113
39	0.004066791887094828
40	0.002895004394203098
41	0.0029639330702555533
42	0.0019300029294687323
43	0.001447502197101549
44	0.0010339301407868207
45	0.001240716168944185
46	0.0011717874928917303
47	0.0018610742534162774
48	0.0015164308731540038
49	4.1357205631472834E-4
50	0.0010339301407868207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1450775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.95839652338242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1363466111736	51.40570182580445
2	13.349637311588467	17.34342067859009
3	3.909871562826059	7.619369620006561
4	1.499696040266222	3.89671400192639
5	0.6912093490992112	2.2449925489727813
6	0.3759684145106456	1.4653383210029953
7	0.22253793195039046	1.0118995057588869
8	0.1466796561911503	0.7622460215022516
9	0.09793610137864703	0.57255948965775
>10	0.4829732821544067	5.724650670508937
>50	0.051534928272111064	2.368064739645092
>100	0.033050180717662074	4.046958490715562
>500	0.0015991436696893612	0.6894023896991166
>1k	9.594862018136168E-4	0.8486816962092087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1991	0.13723699402043735	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1974	0.1360652065275456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	6.892867605245473E-5
11	0.0	0.0	0.0	6.892867605245473E-5	6.892867605245473E-5
12	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
13	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
14	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
15	0.0	0.0	0.0	1.3785735210490946E-4	6.892867605245473E-5
16	0.0	0.0	0.0	3.446433802622736E-4	6.892867605245473E-5
17	0.0	0.0	0.0	4.1357205631472834E-4	6.892867605245473E-5
18	0.0	0.0	0.0	8.960727886819114E-4	6.892867605245473E-5
19	0.0	0.0	0.0	8.960727886819114E-4	6.892867605245473E-5
20	0.0	0.0	0.0	9.650014647343661E-4	1.3785735210490946E-4
21	0.0	0.0	0.0	0.0011717874928917303	1.3785735210490946E-4
22	0.0	0.0	0.0	0.0018610742534162774	2.0678602815736417E-4
23	0.0	0.0	0.0	0.0026192896899932794	2.0678602815736417E-4
24	0.0	0.0	0.0	0.003997863211042374	2.0678602815736417E-4
25	0.0	0.0	0.0	0.004825007323671831	2.757147042098189E-4
26	0.0	0.0	0.0	0.005858937464458651	2.757147042098189E-4
27	0.0	0.0	0.0	0.007857869069979838	4.8250073236718303E-4
28	0.0	0.0	0.0	0.012062518309179577	4.8250073236718303E-4
29	0.0	0.0	0.0	0.020196102083369232	4.8250073236718303E-4
30	0.0	0.0	0.0	0.037910771828850096	4.8250073236718303E-4
31	0.0	0.0	0.0	0.0818872671503162	4.8250073236718303E-4
32	0.0	0.0	0.0	0.138546638865434	5.514294084196378E-4
33	0.0	0.0	0.0	0.18521135255294585	5.514294084196378E-4
34	0.0	0.0	0.0	0.2444900139580569	5.514294084196378E-4
35	0.0	0.0	0.0	0.3122469025176199	5.514294084196378E-4
36	0.0	0.0	0.0	0.40468025710396166	5.514294084196378E-4
37	0.0	0.0	0.0	0.5440540400820251	5.514294084196378E-4
38	0.0	0.0	0.0	0.7340214712825903	5.514294084196378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTT	190	0.0	26.630953	25
TAGGACC	1160	0.0	26.551102	4
TTACGGC	175	0.0	26.39938	18
GGACCGT	215	0.0	24.557562	6
TAGCGCG	45	0.0013985396	24.44387	17
CCGCCGT	210	0.0	24.094673	24
TGTGCGA	175	0.0	23.887623	31
GCCGTTT	215	0.0	23.53433	26
GTATCAA	5835	0.0	23.413332	1
AGGCGTA	85	2.3538632E-7	23.29357	18
TGTTACG	95	2.6870111E-8	23.15735	16
TGCGAAG	180	0.0	22.002516	33
ATAGCGC	60	2.873618E-4	21.999483	16
GTCGGCA	70	3.2165095E-5	21.999483	16
GACCGTT	230	0.0	21.999481	7
GGCCGCC	220	0.0	21.999481	22
TACGGCC	210	0.0	21.998726	19
CCTACCG	80	3.61819E-6	21.998724	3
GTAGGAC	2505	0.0	21.691357	3
ACCGTTA	225	0.0	21.510605	8
>>END_MODULE