FastQCFastQC Report
Thu 2 Feb 2017
SRR4064215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539507
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35790.2324770202408953No Hit
TATCAACGCAGAGTACTTTTTTTTT23100.1500480348579123No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17670.11477700328741604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAC400.00527978914.24970710
ACGGTAT2300.013.2175499
TCGCGTA655.4518074E-513.1540039
CCGTCGT2000.012.8251539
CGTCGTA2050.012.51193710
CGTTATT1001.4500984E-712.3449322
TAGGTTG701.0920895E-412.2128445
CTATTGT1251.4133548E-912.1585654
GCTCGAC550.003068146312.09105411
CGACCAT1900.011.99975410
CGCATCG1950.011.69320813
CCGACCA1950.011.6924489
CGCGCTC658.024725E-411.69206710
CGAGATT907.4701165E-611.6097414
GTGCTAT907.480934E-611.60823251
ACCGTCG2300.011.5657328
TTATACT1251.8344508E-811.3986554
TTAACCA1501.7644197E-1011.3986554
GTATCAA21350.011.3882761
TACCGTC2800.011.1965637
Produced by FastQC (version 0.11.5)