##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064215_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1539507
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220553073159135	32.0	32.0	32.0	32.0	32.0
2	30.761780881801773	32.0	32.0	32.0	32.0	32.0
3	30.784617413236834	32.0	32.0	32.0	32.0	32.0
4	30.81450880054459	32.0	32.0	32.0	32.0	32.0
5	30.720551449262654	32.0	32.0	32.0	32.0	32.0
6	34.38851658355564	36.0	36.0	36.0	32.0	36.0
7	34.31123145266634	36.0	36.0	36.0	32.0	36.0
8	34.27139727198382	36.0	36.0	36.0	32.0	36.0
9	34.42341022158392	36.0	36.0	36.0	32.0	36.0
10	34.076966847179	36.0	36.0	36.0	32.0	36.0
11	34.406453494527796	36.0	36.0	36.0	32.0	36.0
12	34.19589452987223	36.0	36.0	36.0	32.0	36.0
13	34.293939553376504	36.0	36.0	36.0	32.0	36.0
14	34.17895858869105	36.0	36.0	36.0	32.0	36.0
15	34.108487976995235	36.0	36.0	36.0	32.0	36.0
16	34.10859840195595	36.0	36.0	36.0	32.0	36.0
17	34.033288578746316	36.0	36.0	36.0	32.0	36.0
18	34.02973744192134	36.0	36.0	36.0	32.0	36.0
19	34.05413096530253	36.0	36.0	36.0	32.0	36.0
20	34.03403946847919	36.0	36.0	36.0	32.0	36.0
21	34.0142636571318	36.0	36.0	36.0	32.0	36.0
22	33.99462165485444	36.0	36.0	36.0	32.0	36.0
23	33.9348505722936	36.0	36.0	36.0	32.0	36.0
24	33.9127837677906	36.0	36.0	36.0	32.0	36.0
25	33.50477198219949	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	16.0
5	16.0
6	69.0
7	27.0
8	95.0
9	93.0
10	113.0
11	52.0
12	92.0
13	71.0
14	162.0
15	310.0
16	445.0
17	593.0
18	762.0
19	1111.0
20	1727.0
21	2766.0
22	4238.0
23	6662.0
24	9937.0
25	13841.0
26	19000.0
27	24542.0
28	32551.0
29	43300.0
30	56658.0
31	75358.0
32	105676.0
33	148424.0
34	316363.0
35	674430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21281827349947	16.66907093769169	10.63558529053991	26.482525498268927
2	17.195486909790812	19.93292335554714	36.48505651106609	26.386533223595958
3	19.224958070203016	23.77767009336931	28.056717926465986	28.94065390996169
4	12.982103998181168	15.276494852057553	35.39907109682029	36.34233005294099
5	14.967021816336883	36.23312681773033	33.392903892614484	15.4069474733183
6	34.406664375565605	34.90871434798793	16.897151866868406	13.78746940957806
7	30.225604748588836	30.193119328045576	21.04360063203634	18.53767529132925
8	28.28240296719092	32.27333452894285	19.64925755248336	19.79500495138288
9	27.59285808054091	14.07750206986728	18.262403281633226	40.06723656795859
10	15.9189477795911	26.56463971122043	31.646164435111363	25.870248074077107
11	37.50415945261604	21.024733145118102	22.234873890595996	19.236233511669866
12	24.882111572662495	23.458090867197345	28.645376661243745	23.014420898896415
13	29.60387384020409	19.365561170601715	25.02877756292553	26.00178742626867
14	23.77073155512338	19.263543922687127	25.108678978417604	31.85704554377189
15	25.329378173784807	26.697716922280986	22.132796388360443	25.840108515573768
16	25.971328937233707	25.40681254610867	23.371758580681647	25.250099935975978
17	24.338753644703335	25.431222189434877	24.98137837707844	25.248645788783346
18	25.092924720250764	24.706794717949585	25.662238955966878	24.538041605832774
19	25.627318194405262	24.71882650122792	25.215389755677098	24.43846554868972
20	25.92557681769161	24.108909056751205	24.663998372240723	25.301515753316462
21	26.781642220656337	24.04404442144731	24.345080072492973	24.82923328540338
22	26.031718791339447	24.02402343838875	24.66179255309813	25.282465217173677
23	24.779068845229226	23.9958472613898	25.09143435903637	26.133649534344606
24	24.95651111209342	24.629271856904005	25.019241720209788	25.394975310792784
25	25.114897921241457	24.241608713910352	24.94510252079864	25.698390844049555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	130.0
2	344.5
3	559.0
4	559.0
5	559.0
6	1560.5
7	2562.0
8	2562.0
9	2562.0
10	2748.0
11	2934.0
12	2934.0
13	2934.0
14	3365.5
15	3797.0
16	3797.0
17	3797.0
18	6426.5
19	9056.0
20	9056.0
21	9056.0
22	14254.5
23	19453.0
24	19453.0
25	19453.0
26	28888.0
27	38323.0
28	38323.0
29	38323.0
30	48443.0
31	58563.0
32	58563.0
33	58563.0
34	74270.5
35	89978.0
36	89978.0
37	89978.0
38	105302.0
39	120626.0
40	120626.0
41	120626.0
42	139981.5
43	159337.0
44	159337.0
45	159337.0
46	179760.5
47	200184.0
48	200184.0
49	200184.0
50	206822.0
51	213460.0
52	213460.0
53	213460.0
54	201460.0
55	189460.0
56	189460.0
57	189460.0
58	173841.0
59	158222.0
60	158222.0
61	158222.0
62	138235.0
63	118248.0
64	118248.0
65	118248.0
66	97058.0
67	75868.0
68	75868.0
69	75868.0
70	57102.0
71	38336.0
72	38336.0
73	38336.0
74	29129.0
75	19922.0
76	19922.0
77	19922.0
78	15657.0
79	11392.0
80	11392.0
81	11392.0
82	7920.0
83	4448.0
84	4448.0
85	4448.0
86	3413.5
87	2379.0
88	2379.0
89	2379.0
90	1695.5
91	1012.0
92	1012.0
93	1012.0
94	692.0
95	372.0
96	372.0
97	372.0
98	629.0
99	886.0
100	886.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03760944250334685
2	0.004806733584192862
3	0.001883719918129635
4	0.0037024839770134206
5	0.01013311404235252
6	0.014160377315595187
7	0.023059330032276566
8	0.03527103157049627
9	0.04943140888609145
10	0.058070538165789436
11	0.05475778934425111
12	0.0633969186239491
13	0.06378665377942419
14	0.06696949088247081
15	0.06014912566165662
16	0.06638488814925816
17	0.06320205104621154
18	0.07567357602141464
19	0.07359498852554747
20	0.07768720765803598
21	0.07476419399197275
22	0.076388090473119
23	0.08087004476108262
24	0.07684278148783993
25	0.07697269320633164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1539507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.545594056314904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47951687686835	43.03645792121929
2	13.468630736609692	14.962461907802552
3	4.026020953942515	6.70883176709726
4	1.6716251105062065	3.7140563921008116
5	0.8906224348219222	2.4735076111032632
6	0.5553120363727372	1.8507082168167384
7	0.3605247515030147	1.4017893045968184
8	0.24437359117379207	1.0859101034738652
9	0.1945756293277859	0.972703702790388
>10	0.969951206737474	9.475137212719314
>50	0.0656920538320785	2.5186636818149015
>100	0.06411317941345286	7.5160986809259285
>500	0.0065755919207287645	2.422879854541484
>1k	0.002465846970273287	1.86079364299743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3579	0.2324770202408953	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2310	0.1500480348579123	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1767	0.11477700328741604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.495585924584948E-5	0.0
8	0.0	0.0	0.0	6.495585924584948E-5	0.0
9	0.0	0.0	0.0	6.495585924584948E-5	0.0
10	0.0	0.0	0.0	6.495585924584948E-5	0.0
11	0.0	0.0	0.0	6.495585924584948E-5	0.0
12	0.0	0.0	0.0	6.495585924584948E-5	6.495585924584948E-5
13	0.0	6.495585924584948E-5	0.0	6.495585924584948E-5	6.495585924584948E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCAC	40	0.005279789	14.249707	10
ACGGTAT	230	0.0	13.217549	9
TCGCGTA	65	5.4518074E-5	13.154003	9
CCGTCGT	200	0.0	12.825153	9
CGTCGTA	205	0.0	12.511937	10
CGTTATT	100	1.4500984E-7	12.344932	2
TAGGTTG	70	1.0920895E-4	12.212844	5
CTATTGT	125	1.4133548E-9	12.158565	4
GCTCGAC	55	0.0030681463	12.091054	11
CGACCAT	190	0.0	11.999754	10
CGCATCG	195	0.0	11.693208	13
CCGACCA	195	0.0	11.692448	9
CGCGCTC	65	8.024725E-4	11.692067	10
CGAGATT	90	7.4701165E-6	11.609741	4
GTGCTAT	90	7.480934E-6	11.6082325	1
ACCGTCG	230	0.0	11.565732	8
TTATACT	125	1.8344508E-8	11.398655	4
TTAACCA	150	1.7644197E-10	11.398655	4
GTATCAA	2135	0.0	11.388276	1
TACCGTC	280	0.0	11.196563	7
>>END_MODULE