FastQCFastQC Report
Thu 2 Feb 2017
SRR4064215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539507
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTCG500.002580003622.00110443
GTATCAA24800.020.2274781
TATACTG2100.018.8562535
AACGCAG28750.017.2165786
GGCGTTA4600.016.7399742
ATACCGT4150.016.4329596
CGTCGTA3800.015.63084110
ACCGTCG3800.015.6308418
GTACATG46100.015.5587691
GTCCGTA850.002978793615.5306971
TTATACC850.002981373815.5286794
TACATGG46300.015.395022
ATCAACG32700.015.2714493
TCAACGC32950.015.0888174
TACCGTC4100.015.023687
CGGCGTT5150.014.95220641
TATCAAC33850.014.9480812
GCGTTAT5050.014.81262543
ACATGGG47000.014.790793
CAACGCA33850.014.7526275
Produced by FastQC (version 0.11.5)