##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1539507
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294473490539502	32.0	32.0	32.0	32.0	32.0
2	31.40870876196081	32.0	32.0	32.0	32.0	32.0
3	31.476305076885	32.0	32.0	32.0	32.0	32.0
4	31.562115664300325	32.0	32.0	32.0	32.0	32.0
5	31.524058675926774	32.0	32.0	32.0	32.0	32.0
6	35.10816059946463	36.0	36.0	36.0	36.0	36.0
7	35.11880296744347	36.0	36.0	36.0	36.0	36.0
8	35.07902205056554	36.0	36.0	36.0	36.0	36.0
9	35.16443445856368	36.0	36.0	36.0	36.0	36.0
10	35.032309693947475	36.0	36.0	36.0	36.0	36.0
11	35.169397735768655	36.0	36.0	36.0	36.0	36.0
12	35.07203409922787	36.0	36.0	36.0	36.0	36.0
13	35.117482414825005	36.0	36.0	36.0	36.0	36.0
14	35.05654212679774	36.0	36.0	36.0	36.0	36.0
15	35.03092028811821	36.0	36.0	36.0	36.0	36.0
16	35.047287865530976	36.0	36.0	36.0	36.0	36.0
17	35.010659256502244	36.0	36.0	36.0	36.0	36.0
18	35.00107631858771	36.0	36.0	36.0	36.0	36.0
19	35.0059603496444	36.0	36.0	36.0	36.0	36.0
20	35.00257485026051	36.0	36.0	36.0	36.0	36.0
21	34.99768692185226	36.0	36.0	36.0	36.0	36.0
22	34.98041645799597	36.0	36.0	36.0	36.0	36.0
23	34.92454142787269	36.0	36.0	36.0	32.0	36.0
24	34.90209268291732	36.0	36.0	36.0	32.0	36.0
25	34.876266882839765	36.0	36.0	36.0	32.0	36.0
26	34.813568889261305	36.0	36.0	36.0	32.0	36.0
27	34.81316616293398	36.0	36.0	36.0	32.0	36.0
28	34.78194123183591	36.0	36.0	36.0	32.0	36.0
29	34.763927023391254	36.0	36.0	36.0	32.0	36.0
30	34.74271373887874	36.0	36.0	36.0	32.0	36.0
31	34.7350606395424	36.0	36.0	36.0	32.0	36.0
32	34.693946828432736	36.0	36.0	36.0	32.0	36.0
33	34.674592580611844	36.0	36.0	36.0	32.0	36.0
34	34.66153580334484	36.0	36.0	36.0	32.0	36.0
35	34.62280392359372	36.0	36.0	36.0	32.0	36.0
36	34.59743736144103	36.0	36.0	36.0	32.0	36.0
37	34.585054176434404	36.0	36.0	36.0	32.0	36.0
38	34.547935150668366	36.0	36.0	36.0	32.0	36.0
39	34.55314526013847	36.0	36.0	36.0	32.0	36.0
40	34.52913562588543	36.0	36.0	36.0	32.0	36.0
41	34.50988660655651	36.0	36.0	36.0	32.0	36.0
42	34.451237961243436	36.0	36.0	36.0	32.0	36.0
43	34.439224375075916	36.0	36.0	36.0	32.0	36.0
44	34.38082840805531	36.0	36.0	36.0	32.0	36.0
45	34.351528119066685	36.0	36.0	36.0	32.0	36.0
46	34.328215461183355	36.0	36.0	36.0	32.0	36.0
47	34.291604390236614	36.0	36.0	36.0	32.0	36.0
48	34.25505892470772	36.0	36.0	36.0	32.0	36.0
49	34.24295375077866	36.0	36.0	36.0	32.0	36.0
50	33.64759757506786	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	8.0
20	16.0
21	76.0
22	230.0
23	596.0
24	1449.0
25	3172.0
26	6569.0
27	11408.0
28	18364.0
29	27445.0
30	38767.0
31	54060.0
32	76531.0
33	121410.0
34	254259.0
35	925144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09707492852489	17.552182841165767	11.200008834915065	27.150733395394283
2	16.372988874952178	20.571887347767035	36.09375517619693	26.96136860108386
3	18.421062887787233	24.3224441118698	28.437210945863082	28.819282054479885
4	12.593641990585297	15.840850350144558	35.55255026446778	36.01295739480236
5	14.495614505163015	36.678235305198356	33.195042308998914	15.63110788063971
6	34.38319459826373	35.285562567188805	16.712060045274292	13.619182789273177
7	30.170736697834695	30.243422398758042	21.0047385360879	18.581102367319367
8	28.206302407199185	32.432590433171136	19.613226831706516	19.74788032792316
9	27.70576971044919	13.905532228896881	18.1548670726891	40.23383098796483
10	15.762766361696531	26.72441287565078	31.75905085758642	25.75376990506627
11	37.682063153983705	20.936832375559188	22.230038577284805	19.151065893172294
12	24.915297394485496	23.42767540714412	28.68261466030618	22.974412538064207
13	29.68717826467373	19.27349280904292	25.054644258569166	25.98468466771419
14	23.745879357325894	19.107050642901452	25.208882075732138	31.938187924040516
15	25.423513019302412	26.73301092276762	22.122518180253273	25.720957877676696
16	25.95261572278756	25.43004278017899	23.43783493060725	25.179506566426195
17	24.44301974593165	25.413395327205397	24.902777317673774	25.240807609189176
18	25.161058576084994	24.745859375304484	25.665507417333654	24.42757463127687
19	25.717128925038992	24.694139097776105	25.219242263919554	24.36948971326535
20	26.01040725480886	24.042889166178956	24.745583477265065	25.201120101747122
21	26.833135542741932	24.020287013959663	24.45334772755174	24.693229715746664
22	26.122795234315422	23.93527534157061	24.75644784755629	25.185481576557674
23	24.813542631539974	23.859917609292403	25.195616221953447	26.130923537214173
24	25.08372848327379	24.548424813251053	25.051445274439754	25.316401429035402
25	25.23192926433505	24.092062374389243	25.116760356432504	25.559248004843198
26	25.132533767609726	24.825989545406415	25.254073127589045	24.78740355939481
27	25.596478133519895	24.410959776174135	24.66202982829147	25.3305322620145
28	24.958669955314832	24.33987230396567	25.37862868048514	25.32282906023436
29	24.94962552047133	24.594048601141953	25.255315140925124	25.201010737461594
30	24.72147393570272	24.72511161504518	25.442448989667042	25.110965459585056
31	25.28878246894557	24.622546274702902	24.595132889955398	25.49353836639613
32	25.204927217465467	24.501948424403796	24.532738661689997	25.76038569644074
33	24.948114080815365	24.17068599843449	24.974162598615727	25.907037322134418
34	25.484617353335125	24.257110600976183	25.165999550492312	25.092272495196372
35	25.86703822431819	24.256933415179947	25.139707625701135	24.736320734800728
36	25.03071007673784	24.81224791265671	24.753133856958275	25.403908153647176
37	25.73245552780385	24.577493837064658	24.76041690349444	24.92963373163705
38	25.021014981375593	24.396477018639995	25.139959126348433	25.44254887363597
39	25.472545032901788	24.371423189102458	24.660880975425968	25.495150802569782
40	25.60080703046696	24.620080534157513	24.976956370077772	24.802156065297755
41	24.779499744714684	24.820228470613607	25.30202266517869	25.098249119493016
42	25.862161921644617	24.875330713822198	24.782961441999955	24.47954592253323
43	25.196072954598897	24.097778597680513	25.033030676631952	25.673117771088645
44	24.926566399434616	24.595741695604897	24.82555866048798	25.65213324447251
45	25.01461524870606	24.79259338169573	24.937316822216218	25.255474547381983
46	24.89900882824258	24.60020877030135	25.019373286365425	25.481409115090635
47	25.041313080396815	24.538085983173364	25.238719450406116	25.181881486023705
48	25.575943471601338	24.950080352120267	24.59840179178083	24.87557438449756
49	24.869730262722005	25.024715848932573	24.632120340526587	25.473433547818836
50	25.047142143549856	25.137951260577346	24.67773481269483	25.13717178317797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	34.5
2	39.0
3	130.5
4	222.0
5	264.0
6	306.0
7	292.0
8	278.0
9	383.5
10	489.0
11	719.0
12	949.0
13	1665.5
14	2382.0
15	3121.5
16	3861.0
17	3972.0
18	4083.0
19	4207.5
20	4332.0
21	4748.5
22	5165.0
23	5850.5
24	6536.0
25	7421.5
26	8307.0
27	9872.0
28	11437.0
29	13650.5
30	15864.0
31	18328.0
32	20792.0
33	24094.5
34	27397.0
35	31913.0
36	36429.0
37	42997.5
38	49566.0
39	55968.5
40	62371.0
41	69093.5
42	75816.0
43	79765.0
44	83714.0
45	92332.5
46	100951.0
47	107526.0
48	114101.0
49	118902.5
50	123704.0
51	121975.0
52	120246.0
53	118790.5
54	117335.0
55	116772.0
56	116209.0
57	112373.0
58	108537.0
59	99103.0
60	89669.0
61	78922.0
62	68175.0
63	59118.5
64	50062.0
65	42787.5
66	35513.0
67	30355.5
68	25198.0
69	22327.5
70	19457.0
71	15100.5
72	10744.0
73	9179.0
74	7614.0
75	5887.0
76	4160.0
77	3578.5
78	2997.0
79	2494.0
80	1991.0
81	1561.0
82	1131.0
83	936.5
84	742.0
85	524.0
86	306.0
87	223.5
88	141.0
89	98.0
90	55.0
91	48.0
92	41.0
93	31.0
94	21.0
95	23.0
96	25.0
97	18.0
98	11.0
99	8.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010392937479335918
2	5.196468739667959E-4
3	1.9486757773754844E-4
4	0.0
5	0.0
6	1.2991171849169897E-4
7	1.2991171849169897E-4
8	0.0
9	0.0012341613256711401
10	7.794703109501938E-4
11	0.0
12	1.9486757773754844E-4
13	2.5982343698339794E-4
14	1.2991171849169897E-4
15	0.004027263273242668
16	5.846027332126454E-4
17	0.0
18	3.2477929622924743E-4
19	0.0
20	2.5982343698339794E-4
21	0.0
22	6.495585924584948E-5
23	8.444261701960434E-4
24	4.546910147209464E-4
25	0.001883719918129635
26	0.005716115613634755
27	0.00623576248760155
28	0.004546910147209464
29	0.002403366792096431
30	0.004157174991734367
31	0.007340012094780992
32	0.00376743983625927
33	0.004676821865701163
34	0.0029230136660632266
35	0.0027281460883256782
36	0.007145144517043443
37	0.004027263273242668
38	0.008379305842714582
39	0.00500160116193041
40	0.0023384109328505815
41	0.003442660540030023
42	0.0022084992143588823
43	0.001883719918129635
44	0.0013640730441628391
45	0.0012341613256711401
46	0.0010392937479335917
47	0.002793101947571528
48	0.002143543355113033
49	5.846027332126454E-4
50	9.093820294418927E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1539507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.657829923981886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56103178039156	46.08219640826744
2	13.075456874076801	15.33955850995914
3	3.7958728377703137	6.679729899929806
4	1.567281783188155	3.677333931248234
5	0.8236024831950106	2.4155367192111044
6	0.48696567346889696	1.7138609791893515
7	0.32763746564073937	1.3452951915395264
8	0.2349911066576603	1.102725469437866
9	0.16465585765502955	0.8692519764884487
>10	0.8462995448449919	8.713325700644205
>50	0.05589665223597461	2.310331494894222
>100	0.05283927005198695	6.465395097369943
>500	0.006019525737907633	2.313212388332497
>1k	0.0014491450850518375	0.9722462334883087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	1.2991171849169897E-4
14	0.0	0.0	0.0	0.0	1.2991171849169897E-4
15	0.0	0.0	0.0	6.495585924584948E-5	1.9486757773754844E-4
16	0.0	0.0	0.0	1.2991171849169897E-4	2.5982343698339794E-4
17	6.495585924584948E-5	0.0	0.0	1.2991171849169897E-4	2.5982343698339794E-4
18	6.495585924584948E-5	0.0	0.0	2.5982343698339794E-4	2.5982343698339794E-4
19	6.495585924584948E-5	0.0	0.0	3.2477929622924743E-4	2.5982343698339794E-4
20	6.495585924584948E-5	0.0	0.0	3.897351554750969E-4	3.2477929622924743E-4
21	6.495585924584948E-5	0.0	0.0	4.546910147209464E-4	3.897351554750969E-4
22	1.2991171849169897E-4	0.0	0.0	5.846027332126454E-4	3.897351554750969E-4
23	1.2991171849169897E-4	0.0	0.0	9.743378886877422E-4	3.897351554750969E-4
24	1.9486757773754844E-4	0.0	0.0	0.0012991171849169897	3.897351554750969E-4
25	1.9486757773754844E-4	0.0	0.0	0.0016888523403920865	3.897351554750969E-4
26	1.9486757773754844E-4	0.0	0.0	0.002013631636621334	4.546910147209464E-4
27	1.9486757773754844E-4	0.0	0.0	0.002793101947571528	4.546910147209464E-4
28	1.9486757773754844E-4	0.0	0.0	0.005391336317405507	4.546910147209464E-4
29	1.9486757773754844E-4	0.0	0.0	0.011172407790286111	4.546910147209464E-4
30	1.9486757773754844E-4	0.0	0.0	0.023384109328505813	4.546910147209464E-4
31	1.9486757773754844E-4	0.0	0.0	0.05527743621821791	4.546910147209464E-4
32	1.9486757773754844E-4	0.0	0.0	0.08911943888530549	4.546910147209464E-4
33	1.9486757773754844E-4	0.0	0.0	0.12568958764071875	4.546910147209464E-4
34	1.9486757773754844E-4	0.0	0.0	0.16375372115878656	5.196468739667959E-4
35	1.9486757773754844E-4	0.0	0.0	0.20565025037235946	5.196468739667959E-4
36	1.9486757773754844E-4	0.0	0.0	0.2626165389309695	5.196468739667959E-4
37	1.9486757773754844E-4	0.0	0.0	0.35550341765253424	5.196468739667959E-4
38	1.9486757773754844E-4	0.0	0.0	0.4819075197449573	5.846027332126454E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTCG	50	0.0025800036	22.001104	43
GTATCAA	2480	0.0	20.227478	1
TATACTG	210	0.0	18.856253	5
AACGCAG	2875	0.0	17.216578	6
GGCGTTA	460	0.0	16.73997	42
ATACCGT	415	0.0	16.432959	6
CGTCGTA	380	0.0	15.630841	10
ACCGTCG	380	0.0	15.630841	8
GTACATG	4610	0.0	15.558769	1
GTCCGTA	85	0.0029787936	15.530697	1
TTATACC	85	0.0029813738	15.528679	4
TACATGG	4630	0.0	15.39502	2
ATCAACG	3270	0.0	15.271449	3
TCAACGC	3295	0.0	15.088817	4
TACCGTC	410	0.0	15.02368	7
CGGCGTT	515	0.0	14.952206	41
TATCAAC	3385	0.0	14.948081	2
GCGTTAT	505	0.0	14.812625	43
ACATGGG	4700	0.0	14.79079	3
CAACGCA	3385	0.0	14.752627	5
>>END_MODULE