FastQCFastQC Report
Thu 2 Feb 2017
SRR4064214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921514
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33540.3639662555316577No Hit
TATCAACGCAGAGTACTTTTTTTTT23070.25034888238268765No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21160.22962212185598918No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14700.15952009410600382No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12000.13022048498449293No Hit
GGTATCAACGCAGAGTACTTTTTTT11100.12045394861065595No Hit
GTACATGGGGTGGTATCAACGCAAA10160.11025334395353734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA653.3677206E-614.6157189
CCTATAC400.00528946414.244913
GTCGCGT707.2531457E-613.5717388
CGCGTAA707.2572147E-613.57100210
GCGTAAC707.2572147E-613.57100211
GTATTAG852.6995804E-713.4098861
TGTGCGG655.449391E-513.15343210
TCGCCAG1450.013.10588417
TCCAACG604.0809347E-412.66970918
GATAACG905.398997E-712.66626811
CGTCGTA1600.012.46835710
GGGTAGG853.9445586E-612.2923961
TAACGAA951.037266E-612.00092613
ATAACGA951.037266E-612.00092612
GGTCGCG951.0379463E-612.0002757
TCGTCAC1353.6925485E-1011.96583619
CGCATCG1603.6379788E-1211.875916513
AACGCCG1603.6379788E-1211.8733375
GTATAAT802.8674973E-511.8733371
TAACGCC1701.8189894E-1211.7323774
Produced by FastQC (version 0.11.5)