FastQCFastQC Report
Thu 2 Feb 2017
SRR4064214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921514
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCA654.9316767E-420.30724310
CGAAATT550.004481985220.0006428
TACGACG2650.019.9230065
CGACGGT2750.019.1985327
ACGACGG2750.019.1985326
GAGTGCG708.125498E-418.85577
GTGTAGG1901.8189894E-1218.5269091
ACGGTAT2850.018.5259069
GACGGTA2900.018.2055058
CGAATGC2200.017.99960143
GTATCAA26550.017.9817431
CCGGTCC2500.017.599619
AACGCAG27800.017.0922186
CTTTCGT3100.017.03372838
GATATAC1954.9112714E-1116.9236181
TCTAGCG2350.016.85160428
TAGGACC4050.016.8382194
GTAGGAC2750.016.7987163
ATACGAA2251.8189894E-1216.62185340
TTCGTTC3200.016.49963440
Produced by FastQC (version 0.11.5)